Identification of potato cyst nematode species Globodera rostochiensis and G. pallida by PCR using specific primer combinations

in Nematology
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Abstract

RAPD fragments specific for potato cyst nematode (PCN) species Globodera rostochiensis and G. pallida were identified. These were used to derive two primer combinations for PCR which amplify only one specific band in each species. The primer combinations were evaluated on 39 samples of PCN populations containing all currently described pathotypes as well as some South American PCN populations. This method for species identification is highly sensitive and also allows the detection of species mixtures in a sample. Furthermore, the generation of unique amplification products facilitates the application of automatic detection methods without electrophoretic separation and is therefore particularly useful for routine diagnoses. Identification des especes de nematodes a kyste de la pomme de terre Globodera rostochiensis et G. pallida par PCR grace a l'utilisation de combinaisons d'amorces specifiques - Des fragments RAPD specifiques des especes de nematodes a kyste de la pomme de terre (PCN) Globodera rostochiensis et G. pallida, ont ete identifies. A partir de ces fragments, deux combinaisons d'amorces, capables d'amplifier en PCR une seule bande, specifique pour chaque espece, ont ete obtenus. Les combinaisons d'amorces ont ete evaluees sur 39 echantillons de populations de PCN contenant tous les pathotypes decrits ainsi que quelques populations provenant d'Amerique du Sud. Cette methode d'identification d'espece est tres sensible et elle permet de plus l'identification d'un melange d'especes dans un meme echantillon. En sus, la generation de produits uniques d'amplification permet l'utilisation de methodes d'identification automatisees, sans separation electrophoretique, tres utiles donc pour le diagnostic de routine.

Identification of potato cyst nematode species Globodera rostochiensis and G. pallida by PCR using specific primer combinations

in Nematology

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