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Surface coat proteins of the pine wood nematode, Bursaphelenchus xylophilus: profiles of stage- and isolate-specific characters

In: Nematology
Authors:
Ryoji Shinya Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan;, Email: r.shinya@fx5.ecs.kyoto-u.ac.jp

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Yuko Takeuchi Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan

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Natsuko Miura Laboratory of Biomacromolecular Chemistry, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan

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Kouichi Kuroda Laboratory of Biomacromolecular Chemistry, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan

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Mitsuyoshi Ueda Laboratory of Biomacromolecular Chemistry, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan

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Kazuyoshi Futai Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan

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Online Publication Date:
01 Jan 2009
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Abstract

The present study was made to determine the binding patterns of several lectins to the surface coat (SC) proteins of various isolates and developmental stages of the pine wood nematode (PWN), Bursaphelenchus xylophilus. Also, the detailed characteristics of the SC proteins were profiled by using molecular techniques. The lectin-binding study demonstrated the stage-specific characters of SC in binding to the lectin, wheat germ agglutinin (WGA). WGA binding was observed only to the outer surfaces of third-stage propagative juveniles and to the egg shells, and this occurred more frequently in virulent than in avirulent PWN isolates. A greater variety of lectins bound to eggs than to any other life stage. For characterisation, the SC proteins extracted were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and analysed by lectin blotting. The results showed that the carbohydrate and protein patterns of the SC of the PWN changed during nematode development.

Title:
Surface coat proteins of the pine wood nematode, Bursaphelenchus xylophilus: profiles of stage- and isolate-specific characters
Article Type:
Research Article
DOI:
https://doi.org/10.1163/156854109X447006
Language:
English
Pages:
429–438
Keywords:
GLYCAN; WGA; LECTIN BLOT; SDS-PAGE; GLYCOPROTEIN
In:
Nematology
In:
Volume 11: Issue 3
Publisher:
Brill
E-ISSN:
1568-5411
Print ISSN:
1388-5545
Subjects:
Nematology, Biology

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