1 1Plant Pest Diagnostics Center, California Department of Food and Agriculture, 3294 Meadowview Road, Sacramento, CA 95832-1448, USA, Centre of Parasitology, A.N. Severtsov Institute of Ecology and Evolution, Russian Academy
of Sciences, Leninskii Prospect 33, Moscow, 117071, Russia
2 2Laboratory for Electron Microscopy, North West University, Potchefstroom Campus, Potchefstroom, 2520 South Africa
3 3National Collection of Nematodes, Biosystematics Division, ARC-Plant Protection Research Institute, Private Bag X134, Queenswood, 0121 South Africa;, Email: VDBergE@arc.agric.za
Two Hemicycliophora species, H. lutosa and H. typica, found in samples from fallow soil and sugarcane soil in South Africa, were studied morphologically and, for the first time, molecularly. Diagnostic PCR-IT-rRNA-RFLP profiles generated by five restriction enzymes are provided. Study of phylogenetic relationships using D2-D3 expansion segment of 28S rRNA gene sequences revealed that H. lutosa was related to H. poranga. Hemicycliophora lutosa and H. poranga are compared morphologically. SEM photographs are given for H. typica and for H. lutosa for the first time. The male of H. typica represents a first report for South Africa.