Two new thoracostomopsid species, Enoplolaimus pectinatus sp. n., E. patrokli sp. n., and one known species, E. medius, from the Sea of Japan, are described and illustrated with the aid of LM and LSM pictures. Enoplolaimus pectinatus sp. n. belongs to a group of species lacking a preanal supplementary organ and differs from the other species in this group by the shape of the spicules and the gubernaculum, which has a plate-like triangular crura provided with a comb of 14 denticles. Enoplolaimus patrokli sp. n. is characterised by the straight spicules and also differs from other species by the shape of the gubernaculum which has a plate-like cuneus with a dorsal, V-like, thickening and a small proximal dorsal process. Additional information is provided for E. medius on the basis of confocal microscopy. The structure of the head and spicules of E. medius is described. A pictorial key for the genus Enoplolaimus is proposed.