Save

Differentiation of Hemicriconemoides mangiferae and H. litchi (Nematoda: Criconematina) based on morphometrics and nuclear ribosomal DNA sequences

In: Nematology
Authors:
Ruey-Shyang ChenDepartment of Biochemical Science and Technology, National Chiayi University, Chiayi, Taiwan, R.O.C.

Search for other papers by Ruey-Shyang Chen in
Current site
Google Scholar
PubMed
Close
,
Hui-Fang NiDepartment of Plant Protection, Chiayi Agricultural Experiment Station, TARI, Chiayi, Taiwan, R. O. C

Search for other papers by Hui-Fang Ni in
Current site
Google Scholar
PubMed
Close
,
Tung-Tsuan TsayDepartment of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R. O. C

Search for other papers by Tung-Tsuan Tsay in
Current site
Google Scholar
PubMed
Close
, and
Diann-Yih ChenPlant Pathology Division, Taiwan Agricultural Research Institute, Wufeng, Taichung, Taiwan, R. O. C

Search for other papers by Diann-Yih Chen in
Current site
Google Scholar
PubMed
Close
Download Citation Get Permissions

Access options

Get access to the full article by using one of the access options below.

Institutional Login

Log in with Open Athens, Shibboleth, or your institutional credentials

Login via Institution

Purchase

Buy instant access (PDF download and unlimited online access):

$34.95

Abstract

Hemicriconemoides mangiferae and H. litchi, which have been considered as junior synonyms of H. strictathecatus, are morphologically similar, making identification difficult. In the present study, six populations of Hemicriconemoides spp. were collected from mango and litchi in Taiwan and were differentiated based on morphometrics and molecular characteristics. By comparing morphometric data from females and males, three populations collected from mango were identified as H. mangiferae and another three populations from litchi were identified as H. litchi. According to SEM, both of the H. mangiferae and H. litchi males had an areolated lateral field with four incisures; caudal alae were not found. The internal transcribed spacer (ITS) sequences of ribosomal DNA (rDNA) from these nematode populations were sequenced and aligned. The sequence identity of the ITS region was 89-95% between H. mangiferae and H. litchi populations, suggesting rDNA sequence divergence could be a taxonomic aid for species differentiation. Since H. mangiferae and H. litchi are closely related to H. strictathecatus, more rDNA sequence information could be valuable to deal with molecular phylogenetic relationships of these species in depth.

Content Metrics

All Time Past Year Past 30 Days
Abstract Views 251 43 1
Full Text Views 188 4 0
PDF Views & Downloads 66 7 0