Morphological and molecular characterisation of Xiphinema mazandaranense n. sp. (Dorylaimida: Longidoridae), a new member of the Xiphinema pyrenaicum species complex

in Nematology
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Xiphinema mazandaranense n. sp. is described and illustrated. The new species belongs to morphospecies group 6, which is characterised by having two equally developed female genital branches that have spines in the uteri and a short, rounded tail. The new species is characterised by having spines in the tubular portion of the uterus, body 3.7-5.2 mm long, odontostyle 163-173 μm long, odontophore 96-100 μm long, body 62-80 μm diam., rounded tail, four juvenile stages, males rare with three ventromedian supplements next to the adcloacal pair and spicules 85 μm long. The polytomous identification codes of the new species are: A4-B3-C7b-D6-E456-F45-G4-H2-I23-J7b-K2-L1. The new species appears closely related to the members of X. pyrenaicum group which are characterised by a rounded tail with or without an inconspicuous projecting bulge and a uterus devoid of Z-differentiation but showing spiniform structures. The new species differs from members of the X. pyrenaicum group, which includes the recently described X. iranicum, by a more rounded tail without any projection. Beside morphological and morphometric data, molecular analyses of the near-full-length small subunit rDNA gene (SSU) placed the new species in close relationship with some species belonging to Xiphinema morphospecies group 6 and further separated this species from the X. pyrenaicum complex.

Morphological and molecular characterisation of Xiphinema mazandaranense n. sp. (Dorylaimida: Longidoridae), a new member of the Xiphinema pyrenaicum species complex

in Nematology



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    Xiphinema mazandaranense n. sp. A: Anterior region in lateral optical view; B: Female anterior genital branch (fresh female); C-E: Lip region; F-H: Entire body, male (F) and females (G, H); I, J: Female tail. K: Male posterior end; L-O: Tail of juveniles from J1-J4, respectively.

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    Xiphinema mazandaranense n. sp. A: Anterior region (optical section); B: Vulval region; C: Female posterior end; D: Male posterior end; E-H: Tail of juveniles from J1-J4, respectively.

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    Xiphinema mazandaranense n. sp. A: Sparse spines (arrows) in pars dilatata uteri of fresh females; B, C: Spines (arrows) in uterus of fresh females.

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    Xiphinema mazandaranense n. sp. Graph of correlation of functional and replacement odontostyle to body length in juveniles and females. This figure is published in colour in the online edition of this journal, which can be accessed via

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    Bayesian consensus tree inferred from 18S under GTR + I + G model (lnL = 5450.4111; AIC = 10920.8223; freqA = 0.2778; freqC = 0.2167; freqG = 0.2555; freqT = 0.2501; R(a) = 0.7697; R(b) = 1.9004; R(c) = 1.0984; R(d) = 0.3441; R(e) = 3.9075; R(f) = 1; Pinva = 0.723; Shape = 0.6969). Posterior probability values exceeding 50% are given on appropriate clades.


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