A new entomopathogenic nematode, Steinernema xinbinense n. sp. (Nematoda: Steinernematidae), from north China
In: Nematology2Laboratory of Agrozoology, Department of Crop Protection, Ghent University, Coupure Links 653, 9000 Ghent, Belgium
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3Institute of Agriculture and Fisheries Research, Burg. Van Gansberghelaan 96, 9820 Merelbeke, Belgium
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During a survey for entomopathogenic nematodes in northern China, a new species of Steinernema was isolated from soil samples collected from Xinbin county, Liaoning province. This nematode was obtained by the insect-baiting technique using last instar larvae of Galleria mellonella. It is described herein as S. xinbinense n. sp. The nematode can be separated from other described species of the group by morphological and morphometric characteristics of the different stages and by characterisation and phylogeny of DNA sequences of the D2D3 domain of the LSU or ITS regions of rDNA. This new species is characterised by the following morphological characters: infective third-stage juvenile with a body length of 694 (635-744) μm, distance from head to excretory pore of 51 (46-53) μm, tail length of 73 (61-81) μm, E = 71 (65-78)%, presence of eight unevenly spaced and developed ridges in middle lateral field (i.e., nine lines). First generation male with well curved, yellowish spicules 56 (49-62) μm long and gubernaculum 35 (30-41) μm long, small mucron mostly present, first generation female with protruding vulva, tail conical with one or two small mucrons and D = 45 (41-50)%. Cross hybridisation tests with S. tielingense, S. kraussei, S. feltiae and S. hebeiense showed that this species was reproductively isolated. The analyses of ITS-rDNA and D2D3 sequence confirm that the studied nematode isolate is a valid new species belonging to the ‘feltiae-kraussei-oregonense’ group.
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A new entomopathogenic nematode, Steinernema xinbinense n. sp. (Nematoda: Steinernematidae), from north China
In: Nematology2Laboratory of Agrozoology, Department of Crop Protection, Ghent University, Coupure Links 653, 9000 Ghent, Belgium
Search for other papers by Juan Ma in
Current site
Google Scholar
PubMed
Search for other papers by Shulong Chen in
Current site
Google Scholar
PubMed
Search for other papers by Patrick De Clercq in
Current site
Google Scholar
PubMed
Search for other papers by Lieven Waeyenberge in
Current site
Google Scholar
PubMed
Search for other papers by Richou Han in
Current site
Google Scholar
PubMed
3Institute of Agriculture and Fisheries Research, Burg. Van Gansberghelaan 96, 9820 Merelbeke, Belgium
Search for other papers by Maurice Moens in
Current site
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PubMed
During a survey for entomopathogenic nematodes in northern China, a new species of Steinernema was isolated from soil samples collected from Xinbin county, Liaoning province. This nematode was obtained by the insect-baiting technique using last instar larvae of Galleria mellonella. It is described herein as S. xinbinense n. sp. The nematode can be separated from other described species of the group by morphological and morphometric characteristics of the different stages and by characterisation and phylogeny of DNA sequences of the D2D3 domain of the LSU or ITS regions of rDNA. This new species is characterised by the following morphological characters: infective third-stage juvenile with a body length of 694 (635-744) μm, distance from head to excretory pore of 51 (46-53) μm, tail length of 73 (61-81) μm, E = 71 (65-78)%, presence of eight unevenly spaced and developed ridges in middle lateral field (i.e., nine lines). First generation male with well curved, yellowish spicules 56 (49-62) μm long and gubernaculum 35 (30-41) μm long, small mucron mostly present, first generation female with protruding vulva, tail conical with one or two small mucrons and D = 45 (41-50)%. Cross hybridisation tests with S. tielingense, S. kraussei, S. feltiae and S. hebeiense showed that this species was reproductively isolated. The analyses of ITS-rDNA and D2D3 sequence confirm that the studied nematode isolate is a valid new species belonging to the ‘feltiae-kraussei-oregonense’ group.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 399 | 112 | 17 |
| Full Text Views | 217 | 1 | 0 |
| PDF Views & Downloads | 23 | 2 | 0 |
