The use of Pluronic F-127 to study the development of the potato cyst nematode, Globodera pallida

in Nematology
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This paper demonstrates a simple novel in vitro method using Pluronic F-127 aqueous solution to study the development of the potato cyst nematode, Globodera pallida, in Solanum spp. without any need for sterilisation of either the plants or the nematodes. In this study, this method was successfully applied to comparative studies on the development of G. pallida in Solanum tuberosum (potato) or S. sisymbriifolium (sticky nightshade). The protocol described here could be useful for screening transgenic plants or different plant cultivars/species for their ability to allow development not only of G. pallida but also any other plant-parasitic nematodes.


International Journal of Fundamental and Applied Nematological Research



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  • Solanum sisymbriifolium at 6 weeks post inoculation. Scale in cm. This figure is published in colour in the online edition of this journal, which can be accessed via

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  • A: Female of Globodera pallida (arrowed) in the root of Solanum tuberosum at 19 dpi stained with acid fuchsin (scale bar = 0.2 mm); B: Female of G. pallida (arrowed) filled with eggs in the root of Solanum tuberosum at 19 dpi (scale bar = 50 μm); C: Mating between an adult male (arrowed) and an adult female (arrow head) of G. pallida in the root of S. tuberosum at 33 dpi (scale bar = 50 μm); D: Second-stage juvenile (arrowed) of G. pallida in the root of S. sisymbriifolium at 33 dpi stained with acid fuchsin (scale bar = 0.2 mm). This figure is published in colour in the online edition of this journal, which can be accessed via

    View in gallery


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