This paper demonstrates a simple novel in vitro method using Pluronic F-127 aqueous solution to study the development of the potato cyst nematode, Globodera pallida, in Solanum spp. without any need for sterilisation of either the plants or the nematodes. In this study, this method was successfully applied to comparative studies on the development of G. pallida in Solanum tuberosum (potato) or S. sisymbriifolium (sticky nightshade). The protocol described here could be useful for screening transgenic plants or different plant cultivars/species for their ability to allow development not only of G. pallida but also any other plant-parasitic nematodes.
Plant-nematode interactions: Environmental signals detected by the nematode’s chemosensory organs control changes in the surface cuticle and behaviour.
Parasite-Journal de la Société Francaise de Parasitologie15,
Comparison of host recognition, invasion, development and reproduction of Meloidogyne graminicola and M. incognita on rice and tomato.
The structure of syncytia. In:
Cellular and molecular aspects of plant-nematode interactions.
Dordrecht, The Netherlands, Kluwer Academic Publishers, pp.
Physiological integration of roots and shoots in plant defense strategies links above- and belowground herbivory.
Axenizing and culturing endomigratory plant-parasitic nematodes using Pluronic F127, including its effects on population dynamics of Pratylenchus penetrans.
Journal of Nematology28,
Chemotaxis can take plant-parasitic nematodes to the source of a chemo-attractant via the shortest possible routes.
Journal of the Royal Society Interface8,
Signalling and behaviour of potato cyst nematode in the rhizosphere of the trap crop, Solanum sisymbriifolium.
Aspects of Applied Biology103,