The use of Pluronic F-127 to study the development of the potato cyst nematode, Globodera pallida

in Nematology
Restricted Access
Get Access to Full Text
Rent on DeepDyve

Have an Access Token?



Enter your access token to activate and access content online.

Please login and go to your personal user account to enter your access token.



Help

Have Institutional Access?



Access content through your institution. Any other coaching guidance?



Connect

This paper demonstrates a simple novel in vitro method using Pluronic F-127 aqueous solution to study the development of the potato cyst nematode, Globodera pallida, in Solanum spp. without any need for sterilisation of either the plants or the nematodes. In this study, this method was successfully applied to comparative studies on the development of G. pallida in Solanum tuberosum (potato) or S. sisymbriifolium (sticky nightshade). The protocol described here could be useful for screening transgenic plants or different plant cultivars/species for their ability to allow development not only of G. pallida but also any other plant-parasitic nematodes.

Nematology

International Journal of Fundamental and Applied Nematological Research

Sections

References

BohorquezM.KochC.TrygstadT.PanditN. (1999). A study of the temperature-dependent micellization of pluronic F127. Journal of Colloid and Interface Science 216, 34-40.

BrodieB.B.EvansK.FrancoJ. (1993). Nematode parasites of potatoes. In: EvansK.TrudgillD.L.WebsterJ.M. (Eds). Plant parasitic nematodes in temperate agriculture. Wallingford, UK, CABI Publishing, pp.  87-132.

ByrdD.W.KirkpatrickT.BarkerK.R. (1983). An improved technique for clearing and staining plant tissues for detection of nematodes. Journal of Nematology 15, 142-143.

ClarkeA.J.PerryR.N. (1977). Hatching of cyst-nematodes. Nematologica 23, 350-368.

CurtisR.H.C. (2008). Plant-nematode interactions: Environmental signals detected by the nematode’s chemosensory organs control changes in the surface cuticle and behaviour. Parasite-Journal de la Société Francaise de Parasitologie 15, 310-316.

DuttaT.K.PowersS.J.KerryB.R.GaurH.S.CurtisR.H.C. (2011). Comparison of host recognition, invasion, development and reproduction of Meloidogyne graminicola and M. incognita on rice and tomato. Nematology 13, 509-520.

ForrestJ.M.S.SpiegelY.RobertsonW.M. (1988). A possible role for the amphids of potato cyst nematode Globodera rostochiensis in host finding. Nematologica 34, 173-181.

GardenerS.JonesJ.G. (1984). A new solidifying agent for culture media which liquefies on cooling. Journal of General Microbiology 130, 731-733.

GolinowskiW.SobczakM.KurekW.GrymaszewskaG. (1997). The structure of syncytia. In: FenollC.GrundlerF.M.W.OhlS.A. (Eds). Cellular and molecular aspects of plant-nematode interactions. Dordrecht, The Netherlands, Kluwer Academic Publishers, pp.  80-97.

HeungensK.MugnieryD.VanmontaguM.GheysenG.NiebelA. (1996). A method to obtain disinfected Globodera infective juveniles directly from cysts. Fundamental and Applied Nematology 19, 91-93.

HongS.C.DonaldsonJ.GrattonC. (2010). Soybean cyst nematode effects on soybean aphid preference and performance in the laboratory. Environmental Entomology 39, 1561-1569.

HusseyR.S. (1989). Disease-inducing secretions of plant-parasitic nematodes. Annual Review of Phytopathology 27, 123-141.

JonesM.G.K. (1981). Host cell responses to endoparasitic nematode attack: structure and function of giant cells and syncytia. Annals of Applied Biology 97, 353-372.

KaplanI.HalitschkeR.KesslerA.RehillB.J.SardanelliS.DennoR.F. (2008). Physiological integration of roots and shoots in plant defense strategies links above- and belowground herbivory. Ecology Letters 11, 841-851.

KoM.P.Van GundyS.D. (1988). An alternative gelling agent for culture and studies of nematodes, bacteria, fungi, and plant tissues. Journal of Nematology 20, 478-485.

KoM.P.SchmittD.P.SipesB.S. (1996). Axenizing and culturing endomigratory plant-parasitic nematodes using Pluronic F127, including its effects on population dynamics of Pratylenchus penetrans. Journal of Nematology 28, 115-123.

PerryR.N. (2002). Hatching. In: LeeD.L. (Ed.). The biology of nematodes. London, UK, Taylor & Francis, pp.  147-169.

ReynoldsA.M.DuttaT.K.CurtisR.H.C.PowersS.J.GaurH.S.KerryB.R. (2011). Chemotaxis can take plant-parasitic nematodes to the source of a chemo-attractant via the shortest possible routes. Journal of the Royal Society Interface 8, 568-577.

SalehH.M.FattahF.A. (1990). Studies on the wheat seed gall nematode. Nematologia Mediterranea 18, 59-62.

Sasaki-CrawleyA.CurtisR.BirkettM.PowersS.PapadopoulosA.PickettJ.BlackshawR.KerryB. (2010). Signalling and behaviour of potato cyst nematode in the rhizosphere of the trap crop, Solanum sisymbriifolium. Aspects of Applied Biology 103, 45-51.

WangC.L.LowerS.WilliamsonV.M. (2009). Application of Pluronic gel to the study of root-knot nematode behaviour. Nematology 11, 453-464.

WurstS.Van Der PuttenW.H. (2007). Root herbivore identity matters in plant-mediated interactions between root and shoot herbivores. Basic and Applied Ecology 8, 491-499.

Figures

  • Solanum sisymbriifolium at 6 weeks post inoculation. Scale in cm. This figure is published in colour in the online edition of this journal, which can be accessed via http://www.brill.com/nemy

    View in gallery
  • A: Female of Globodera pallida (arrowed) in the root of Solanum tuberosum at 19 dpi stained with acid fuchsin (scale bar = 0.2 mm); B: Female of G. pallida (arrowed) filled with eggs in the root of Solanum tuberosum at 19 dpi (scale bar = 50 μm); C: Mating between an adult male (arrowed) and an adult female (arrow head) of G. pallida in the root of S. tuberosum at 33 dpi (scale bar = 50 μm); D: Second-stage juvenile (arrowed) of G. pallida in the root of S. sisymbriifolium at 33 dpi stained with acid fuchsin (scale bar = 0.2 mm). This figure is published in colour in the online edition of this journal, which can be accessed via http://www.brill.com/nemy

    View in gallery

Information

Content Metrics

Content Metrics

All Time Past Year Past 30 Days
Abstract Views 17 17 13
Full Text Views 8 8 8
PDF Downloads 4 4 4
EPUB Downloads 0 0 0