Nematode-antagonistic effects of Cinnamomum aromaticum extracts and a purified compound against Meloidogyne incognita

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The potential use of Cinnamomum aromaticum and its active compound to control Meloidogyne incognita was investigated in vitro and in pot experiments. One compound, cinnamyl acetate, was isolated by thin layer chromatography and silica gel column chromatography, and identified by 1H-NMR, 13C-NMR and mass spectrometry. Juvenile movement and hatch inhibition by cinnamyl acetate was dependent on both the concentration and incubation time of the cinnamyl acetate. Treatment with 25, 50, 75, 100 and 125 μg ml−1 of cinnamyl acetate resulted in 33.7, 65.1, 81.1, 100 and 100% inhibition of movement of second-stage juveniles, respectively, at 50 min after incubation. The juvenile movement inhibition was <20% at the tested concentrations at 10 min after incubation. Cinnamyl acetate treatment resulted in 20.8, 39.4, 81.3 and 90.7% hatch inhibition at 25, 50, 100 and 200 μg ml−1, respectively, at 3 days after incubation and 21.6, 39.3, 73.2 and 88.7% hatch inhibition at 25, 50, 100 and 200 μg ml−1, respectively at 6 days after incubation. In pot tests, C. aromaticum crude extracts effectively inhibited infection of M. incognita on cucumber plants. Cinnamomum aromaticum crude extracts applied at 0.5 and 1.0 mg (g soil)−1 significantly reduced the numbers of galls caused by M. incognita. The activities of pathogenesis-related proteins as β-1,3-glucanase and peroxidase on leaves of plants treated with C. aromaticum crude extracts were significantly higher than those on leaves of control plants.


International Journal of Fundamental and Applied Nematological Research



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  • Isolation of cinnamyl acetate from Cinnamomum aromaticum bark.

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  • A: 1H-NMR; B: 13C-NMR spectra of cinnamyl acetate purified from Cinnamomum aromaticum bark.

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  • Juvenile movement inhibition of cinnamyl acetate purified from Cinnamomum aromaticum with various concentrations (0, 25, 50, 75, 100 and 125 μg ml−1) at 10, 20, 30, 40 and 50 min after incubation. Values are the mean ± SD.

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  • Light microscope images of second-stage juveniles of Meloidogyne incognita after treatment with cinnamyl acetate purified from Cinnamomum aromaticum. A: Untreated; B: Treated with 125 μg ml−1 of cinnamyl acetate for 40 min.

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  • Hatch inhibition of Meloidogyne incognita of cinnamyl acetate purified from Cinnamomum aromaticum by different concentrations (0, 25, 50, 100 and 200 μg ml−1) using direct-contact bioassay at 3 and 6 days after incubation. Means within a block followed by the same letter are not significantly different (P0.05). Values are the mean ± SD.

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  • A: Chitinase; B: β-1,3-Glucanase; C: Peroxidase activity of cucumber leaves of plants at 0, 14, and 28 days after treatment. Un-inoculated (1): not inoculated with Meloidogyne incognita second-stage juveniles (J2). Control (2): Inoculated with 5000 Meloidogyne incognita J2 without Cinnamomum aromaticum crude extract. Means within a block followed by the same letter are not significantly different (P0.05). Values are the mean ± SD.

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