Changes in the pre-parasitic developmental stage of Globodera rostochiensis in response to green manures
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The sequence of events during the pre-parasitic and parasitic stages of the life cycle of Globodera rostochiensis, starting from hatching until host invasion and infection, is of major importance. Many of the physiological changes are initiated in the pre-parasitic stage in response to the influences exerted by the root diffusates of the host plant. The objectives of this study were to investigate whether root diffusates and extracts of green manures from Brassicaceae, in particular yellow mustard, fodder radish and rapeseed, have a direct effect on the pre-parasitic stage of G. rostochiensis. Unhatched second-stage juveniles (J2) were exposed to root diffusates and extracts from roots or above-ground parts of the green manures to assess: i) changes in eggshell permeability; ii) changes in diameter of the dorsal gland nucleolus; and iii) the activation of transcription in the dorsal and subventral pharyngeal glands. The 24 h exposure of unhatched J2 to the green manures did not indicate an alteration in eggshell permeability. The diameter of the dorsal gland nucleolus increased significantly after exposure to diffusates but not to extracts of the green manures, although the size in that case still did not exceed the measurements in J2 exposed to tomato root diffusates. The expression of the gene IVg9 in the dorsal gland was not detected after any treatment. However, transcriptional activation in the subventral pharyngeal glands was observed. Although the maximum expression of the cellulase gene was found in J2 exposed to tomato root diffusates, the expression in J2 exposed to root extracts of green manures was higher than in J2 exposed to root diffusates and extracts of above-ground plant parts. The potential of the investigated brassicaceous green manures to influence the pre-parasitic stage of G. rostochiensis is discussed.
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-
Atkinson H.J., Taylor J.D., Fowler M. (1987). Changes in the second stage juveniles of Globodera rostochiensis prior to hatching in response to potato root diffusate. Annals of Applied Biology 110, 105-114.
-
Blair L., Perry R.N., Oparka K., Jones J.T. (1999). Activation of transcription during the hatching process of the potato cyst nematode Globodera rostochiensis. Nematology 1, 103-111.
-
Blanchard A., Fouville D., Esquibet M., Mugniery D., Grenier E. (2007). Sequence polymorphism of 2 pioneer genes expressed in phytoparasitic nematodes showing different host ranges. Journal of Heredity 98, 611-619.
-
Charlson D.V., Harkins K.R., Tylka G.L. (2008). Relationship between juvenile hatching and acridine orange fluorescence of Heterodera glycines eggs. Nematology 10, 603-610.
-
Davis E.L., Haegeman A., Kikuchi T. (2011). Degradation of the plant cell wall by nematodes. In: Jones J., Gheysen G., Fenoll C. (Eds). Genomics and molecular genetics of plant-nematode interactions. Dordrecht, The Netherlands, Springer, pp. 255-272.
-
Donald P.A., Niblack T.L. (1994). Distinguishing between viable and nonviable Heterodera glycines eggs. Journal of Nematology 26, 596-598.
-
Grenier E., Blok V.C., Jones J.T., Fouville D., Mugniery D. (2002). Identification of gene expression differences between Globodera pallida and G. mexicana by suppression subtractive hybridization. Molecular Plant Pathology 3, 217-226.
-
Holz R.A., Riga E., Atkinson H.J. (1998). Seasonal changes in the dorsal pharyngeal gland nucleolus of unhatched second-stage juveniles of Globodera spp. in Bolivia. Journal of Nematology 30, 291-298.
-
Hoogewijs D., Houthoofd K., Matthijssens F., Vandesompele J., Vanfleteren J. (2008). Selection and validation of a set of reliable reference genes for quantitative sod gene expression analysis in C. elegans. BMC Molecular Biology 9, 9.
-
Jones J.T., Perry R.N., Johnston M.R.L. (1993). Changes in the ultrastructure of the cuticle of the potato cyst-nematode, Globodera rostochiensis, during development and infection. Fundamental and Applied Nematology 16, 433-445.
-
Jones J.T., Robertson L., Perry R.N., Robertson W.M. (1997). Changes in gene expression during stimulation and hatching of the potato cyst nematode Globodera rostochiensis. Parasitology 114, 309-315.
-
Jones J.T., Kumar A., Pylypenko L.A., Thirugnanasambandam A., Castelli L., Chapman S., Cock P.J.A., Grenier E., Lilley C.J., Phillips M.S. et al. (2009). Identification and functional characterization of effectors in expressed sequence tags from various life cycle stages of the potato cyst nematode Globodera pallida. Molecular Plant Pathology 10, 815-828.
-
Lilley C.J., Atkinson H.J., Urwin P.E. (2005). Molecular aspects of cyst nematodes. Molecular Plant Pathology 6, 577-588.
-
von Mende N. (1997). Invasion and migration behaviour of sedentary nematodes. In: Fenoll C., Grundler F.M.W., Ohl S.A. (Eds). Cellular and molecular aspects of plant–nematode interactions. Dordrecht, The Netherlands, Kluwer Academic Publishers, pp. 51-64.
-
Perry R.N. (1986). Physiology of hatching. In: Lamberti F., Taylor C.E. (Eds). NATO advanced study institute on cyst nematodes. New York, Plenum Press, pp. 119-131.
-
Perry R.N. (1989). Dormancy and hatching of nematode eggs. Parasitology Today 5, 377-383.
-
Perry R.N. (1997). Plant signals in nematode hatching and attraction. In: Fenoll C., Grundler F.M.W., Ohl S.A. (Eds). Cellular and molecular aspects of plant-nematode interactions. Dordrecht, The Netherlands, Kluwer Academic Publishers, pp. 38-50.
-
Perry R.N., Beane J. (1982). The effect of brief exposures to potato root diffusate on the hatching of Globodera rostochiensis. Revue de Nématologie 5, 221-224.
-
Perry R.N., Feil J.J. (1986). Observations on a novel hatching bioassay for Globodera rostochiensis using fluorescence microscopy. Revue de Nématologie 9, 280-282.
-
Perry R.N., Zunke U., Wyss U. (1989). Observations on the response of the dorsal and subventral oesophageal glands of Globodera rostochiensis to hatching stimulation. Revue de Nématologie 12, 91-96.
-
Rozen S., Skaletsky H. (2000). Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S., Misener S. (Eds). Bioinformatics methods and protocols: methods in molecular biology. Totowa, NJ, USA, Humana Press, pp. 365-368.
-
Smant G., Goverse A., Stokkermans J.P.W.G., de Boer J.M., Pomp H., Zilverentant J.F., Overmars H.A., Helder J., Schots A., Bakker J. (1997). Potato root diffusate-induced secretion of soluble, basic proteins originating from the subventral esophageal glands of potato cyst nematodes. Phytopathology 87, 839-845.
-
Turner S.J., Rowe J.A. (2006). Cyst nematodes. In: Perry R.N., Moens M. (Eds). Plant nematology. Wallingford, UK, CABI Publishing, pp. 91-122.
-
Turner S.J., Fleming C.C., Moreland B.P., Martin T.J.G. (2009). Variation in hatch among pathotypes of the potato cyst nematodes, Globodera rostochiensis and G. pallida, in response to potato root diffusate from Solanum spp. I. Preliminary assessments to establish optimal testing conditions. Nematology 11, 749-756.
-
Valdes Y., Viaene N., Perry R.N., Moens M. (2011). Effect of the green manures Sinapis alba, Brassica napus and Raphanus sativus on hatching of Globodera rostochiensis. Nematology 13, 965-975.
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Changes in the pre-parasitic developmental stage of Globodera rostochiensis in response to green manures
In: NematologySearch for other papers by Yirina Valdes in
Current site
Google Scholar
PubMed
Search for other papers by Nicole Viaene in
Current site
Google Scholar
PubMed
Search for other papers by Vivian Blok in
Current site
Google Scholar
PubMed
Search for other papers by Juan E. Palomares-Rius in
Current site
Google Scholar
PubMed
3Ghent University, Laboratory for Agrozoology, Coupure links 653, 9000 Ghent, Belgium
Search for other papers by Maurice Moens in
Current site
Google Scholar
PubMed
The sequence of events during the pre-parasitic and parasitic stages of the life cycle of Globodera rostochiensis, starting from hatching until host invasion and infection, is of major importance. Many of the physiological changes are initiated in the pre-parasitic stage in response to the influences exerted by the root diffusates of the host plant. The objectives of this study were to investigate whether root diffusates and extracts of green manures from Brassicaceae, in particular yellow mustard, fodder radish and rapeseed, have a direct effect on the pre-parasitic stage of G. rostochiensis. Unhatched second-stage juveniles (J2) were exposed to root diffusates and extracts from roots or above-ground parts of the green manures to assess: i) changes in eggshell permeability; ii) changes in diameter of the dorsal gland nucleolus; and iii) the activation of transcription in the dorsal and subventral pharyngeal glands. The 24 h exposure of unhatched J2 to the green manures did not indicate an alteration in eggshell permeability. The diameter of the dorsal gland nucleolus increased significantly after exposure to diffusates but not to extracts of the green manures, although the size in that case still did not exceed the measurements in J2 exposed to tomato root diffusates. The expression of the gene IVg9 in the dorsal gland was not detected after any treatment. However, transcriptional activation in the subventral pharyngeal glands was observed. Although the maximum expression of the cellulase gene was found in J2 exposed to tomato root diffusates, the expression in J2 exposed to root extracts of green manures was higher than in J2 exposed to root diffusates and extracts of above-ground plant parts. The potential of the investigated brassicaceous green manures to influence the pre-parasitic stage of G. rostochiensis is discussed.
| All Time | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 190 | 48 | 11 |
| Full Text Views | 66 | 5 | 1 |
| PDF Views & Downloads | 17 | 0 | 0 |