Liquid culture of Panagrolaimus sp. for use as food for marine aquaculture shrimp and fish species

in Nematology
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Free-living nematodes have potential to be used as live food for early life stages of several species in marine aquaculture. Panagrolaimus sp. displays several favourable characteristics for this application. The present study proved the feasibility of propagation in monoxenic liquid culture on Saccharomyces cerevisiae. The development of yeast cell density, nematode numbers and size distribution was assessed daily for 15 days. After a lag phase of 4 days the inoculated first-stage juveniles started development to adults. Yields in terms of nematode number as well as biomass were highly variable. The maximum number of nematodes varied from 45 000 to 238 000 ml−1 and maximum biomass from 49 to 143 g l−1. Information on size, dry and wet weight of the nematodes is provided. The size spectrum of Panagrolaimus sp. individuals ranged from 176 × 8 μm to 1377 × 61 μm and 8.15 to 3202.39 ng wet weight. Water content of the nematodes was 71.7 ± 2.5%, so dry weight per individual was 2.31-905.95 ng. Differentiation of juvenile stages by body length was not possible. Based on comparison of dry weight per individual the Panagrolaimus sp. might be used as a substitute for rotifers, a commonly used live food organism.

Liquid culture of Panagrolaimus sp. for use as food for marine aquaculture shrimp and fish species

in Nematology

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Figures

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    Cubic regression of total length (TL in μm) and maximum body width (MBW in μm) data of 291 individuals of Panagrolaimus sp.

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    Total body length values (in μm) of Panagrolaimus sp. obtained during Experiment 1 (A) (n=14978 nematodes) and Experiment 2 (B) (n=7540 nematodes).

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    Mean population dynamics (n=5) including daily average values of yeast cell density, nematode number (×1000 individuals ml−1), nematode biomass (g l−1) and nematode total length size classes (in μm) over a cultivation period of 15 days in liquid culture at 25°C of Experiment 1 (A) and Experiment 2 (B).

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    Population development of Panagrolaimus sp. from two different flask cultures (A and B) including daily yeast cell density, nematode number (×1000 individuals ml−1), nematode biomass (g l−1) and nematode total length size classes (in μm) over a cultivation period of 15 days in liquid culture at 25°C.

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