Free-living nematodes have potential to be used as live food for early life stages of several species in marine aquaculture. Panagrolaimus sp. displays several favourable characteristics for this application. The present study proved the feasibility of propagation in monoxenic liquid culture on Saccharomyces cerevisiae. The development of yeast cell density, nematode numbers and size distribution was assessed daily for 15 days. After a lag phase of 4 days the inoculated first-stage juveniles started development to adults. Yields in terms of nematode number as well as biomass were highly variable. The maximum number of nematodes varied from 45 000 to 238 000 ml−1 and maximum biomass from 49 to 143 g l−1. Information on size, dry and wet weight of the nematodes is provided. The size spectrum of Panagrolaimus sp. individuals ranged from 176 × 8 μm to 1377 × 61 μm and 8.15 to 3202.39 ng wet weight. Water content of the nematodes was 71.7 ± 2.5%, so dry weight per individual was 2.31-905.95 ng. Differentiation of juvenile stages by body length was not possible. Based on comparison of dry weight per individual the Panagrolaimus sp. might be used as a substitute for rotifers, a commonly used live food organism.
BarrièreA.FélixM. (2006). Isolation of C. elegans and related nematodes. WormBook. Available online at http://www.wormbook.org/chapters/www_nematodeisolation/nematodeisolation.html (accessed 4 July 2012).
Use of the nematode Panagrellus redivivus as an Artemia replacement in a larval penaeid diet.
Journal of the World Aquaculture Society2061-71.
Monoxenic liquid culture of the entomopathogenic nematode Steinernema carpocapsae using a culture medium containing whey: kinetics and modeling.
Journal of Biotechnology12575-84.
Dos SantosG.A.P.DeryckeS.Fonsêca-GenevoisV.G.CoelhoL.C.B.B.CorreiaM.T.S.MoensT. (2008).
Differential effects of food availability on population growth and fitness of three species of estuarine, bacterial-feeding nematodes.
Journal of Experimental Marine Biology and Ecology35527-40.
FockenU.SchlechtriemC.Von WuthenauM.Garcia-OrtegaA.Puello-CruzA.BeckerK. (2006).
Panagrellus redivivus mass produced on solid media as live food for Litopenaeus vannamei larvae.
Life cycle and population development of the entomopathogenic nematodes Steinernema carpocapsae and Steinernema feltiae (Nematoda, Rhabditida) in monoxenic liquid culture.
IvlevaI. (1969). [
Mass cultivation of invertebrates biology and methods.] Academy of Sciences of the USSR, All-Union Hydrobiological Society(translated from Russion by Mercado A. (1973) Jerusalem Keter Press 148 pp.).
Larval pigmentation, survival and growth of Penaeus indicus fed the nematode Panagrellus redivivus enriched with astaxanthin and various lipids.
Molecular evolution in Panagrolaimus nematodes: origins of parthenogenesis, hermaphroditism and the antarctic species P. davidi.
BMC Evolutionary Biology915.
Life cycle and calculation of the intrinsic rate of natural increase of two bacterivorous nematodes, Panagrolaimus sp. and Poikilolaimus sp. from chemoautotrophic Movile Cave, Romania.
Desiccation survival and water contents of entomopathogenic nematodes, Steinernema spp. (Rhabditida: Steinernematidae).
International Journal for Parasitology2761-70.
Development of low-cost technology for mass production of the free-living nematode Panagrellus redivivus as an alternative live food for first feeding fish larvae.
Applied Microbiology and Biotechnology60556-559.
Food dependence and energetics of freeliving nematodes. I. Respiration, growth and reproduction of Caenorhabditis briggsae (Nematoda) at different levels of food supply.
The anhydrobiotic potential and molecular phylogenetics of species and strains of Panagrolaimus (Nematoda, Panagrolaimidae).
Journal of Experimental Biology2082433-2445.
StiernagleT. (2006). Maintenance of C. elegans. In: The C. elegans research community (Ed.) WormBook. The C. elegans Research Community. Available online at www.wormbook.org/Chapters/www_strainmaintain/strainmaintain.html (accessed 04 July 2012).
Culture conditions define automictic or amphimictic reproduction in entomopathogenic rhabditid nematodes of the genus Heterorhabditis.
Fundamental and Applied Nematology17575-582.
Studies of the life-history and energetics of marine and brackish-water nematodes. I. Demography of Monhystera disjuncta at different temperature and feeding conditions.
Monoxenic culture of the slug parasite Phasmarhabditis hermaphrodita (Nematoda: Rhabditidae) with different bacteria in liquid and solid phase.
Fundamental and Applied Nematology18159-166.