1 Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju 500-757, South Korea
2 Western Highlands Agriculture Forestry Science Institute, 53 Nguyen Luong Bang Street, Buon Ma Thuot, Vietnam
3 Plant Protection Department, Faculty of Agriculture and Forestry, Tay Nguyen University, Buon Ma Thuot, Vietnam
The nematicidal activity of Terminalia nigrovenulosa bark (TNB) and its purified compound were assayed against Meloidogyne incognitain vitro. The nematicidal compound was isolated from TNB using silica gel column and Sephadex LH-20 chromatography combined with thin-layer chromatography and high performance liquid chromatography. Structural identification of the nematicidal compound was conducted using 1H-nuclear magnetic resonance (NMR), 13C-NMR and liquid chromatography-tandem mass spectrometry. We found that the nematicidal compound purified from TNB was gallic acid (GA) or 3,4,5-trihydroxy benzoic acid. Nematicidal activity bioassays revealed that GA treatment resulted in 20.3, 37.5, 73.3, 88.3 and 95.8% hatch inhibition at 0, 0.25, 0.5, 1.0 and 2.0 mg ml−1 after 3 days, respectively, of incubation. Eggshells appeared to be deformed and destroyed at 2 and 3 days after incubation with a GA concentration of 1.0 mg ml−1, respectively. Additionally, after treatment with a GA concentration of 1.0 mg ml−1, mortality of second-stage juveniles of M. incognita was 65.0, 75.0, 96.7 and 100% at 3, 6, 9 and 12 h incubation, respectively.
Comparative bioactivity of selected extracts from Meliaceae and some commercial botanical insecticides against two noctuid caterpillars, Trichoplusiani and Pseudaletia unipuncta.
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Plantago lanceolata and Plantago rugelii extracts are toxic to Meloidogyne incognita but not to certain microbes.
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