Axonchoides smokyensis sp. n. (Dorylaimida: Belondiridae) from the Great Smoky Mountains National Park: the second species of a very rare genus

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Axonchoides smokyensis sp. n., collected from a natural forest area in Tennessee, USA, is described and illustrated, including LM and SEM pictures as well as molecular (rRNA SSU-ITS1 and LSU D2/D3) sequences. The new species is characterised by its body length of 2.56-3.18 mm, lip region continuous and 10-12 μm wide, odontostyle 10-13 μm long, neck 662-789 μm long, anterior portion of pharynx enlarging gradually, pharyngeal expansion 407-548 μm long or 60-70% of the total neck length and surrounded by a strong muscular sheath with nearly longitudinal bands, female genital system pseudodidelphic-monodelphic with anterior branch lacking a functional ovary and posterior one bearing a long and tripartite uterus, V = 42-46, female tail slightly clavate (27-34 μm, c = 84-105, c′ = 0.8-1.0), male tail short and rounded (34-38 μm, c = 87-113, c′ = 0.7-0.9), spicules 49-53 μm long, and five or six widely spaced ventromedian supplements outside the range of the spicules. The taxonomy of Axonchoides is discussed on the base of morphological and molecular evidence, and an emended diagnosis proposed. It was easily differentiated from other sequenced dorylaimid taxa, but phylogenetic analysis from SSU and LSU failed satisfactorily to resolve its placement in Dorylaimina.

Axonchoides smokyensis sp. n. (Dorylaimida: Belondiridae) from the Great Smoky Mountains National Park: the second species of a very rare genus

in Nematology

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References

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Figures

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    Axonchoides smokyensis sp. n. A: Anterior region in median view; B: Lip region in surface, lateral view; C: Male, entire; D: Female, entire; E: Pharyngeal expansion; F: Female genital system; G: Female posterior body region; H: Male posterior body region; I: Vagina; J: Spicule; K: Lateral guiding piece.

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    Axonchoides smokyensis sp. n. (LM). A: Female, entire; B: Male, entire; C, D: Anterior region in median view; E: Neck region; F, G: Pharyngeal enlargement; H: Lip region in sublateral view; I: Female, posterior body region; J: Female, caudal region; K: Pharyngo-intestinal junction. (Scale bars: A, B = 500 μm; C, D = 10 μm; E = 50 μm; F, G, J, K = 20 μm; H = 5 μm; I = 200 μm.)

  • View in gallery

    Axonchoides smokyensis sp. n. (LM). A: Female, genital system; B: Vagina; C: Male, posterior body region; D: Oviduct-uterus junction; E: Male, caudal region; F: Spicules; G: Lateral guiding pieces. (Scale bars: A = 100 μm; B, E = 10 μm; C = 50 μm; D, F = 20 μm; G = 5 μm.)

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    Axonchoides smokyensis sp. n. (SEM). A: Lip region in sublateral view; B: Same in lateral view; C: Same in ventral, subfrontal view; D: Same in frontal view; E: Mid-body region, showing fine transverse striation; F: Female caudal region in sublateral view; G: Same in ventral view.

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    Axonchoides smokyensis sp. n. (sequences). A: Nearly-full-length SSU-ITS1 rDNA of 2296 bp; B: LSU D2/D3 rDNA gene sequence of 812 bp.

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    Bayesian 50% majority rule consensus trees as inferred from SSU-ITS1 rDNA gene sequence alignments under the GTR + I + G model. Posterior probabilities are given for appropriate clades. Newly obtained sequence indicated by bold letters.

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    Bayesian 50% majority rule consensus trees as inferred from D2/D3 expansion segments of LSU rDNA gene sequence alignments under the GTR + I + G model. Posterior probabilities are given for appropriate clades. Newly obtained sequence indicated by bold letters.

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