Studies on Ironus Bastian, 1865 (Enoplida: Ironidae) from Iran with a comment on its phylogenetic position

in Nematology
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During a survey on the soil nematodes from Kerman province, three species of Ironus, i.e., I. andamanus, I. elegans and I. ignavus, were isolated from natural areas in Kerman province (Iran). Descriptions, measurements and illustrations are provided for all three species. Furthermore, sequence data of the 18S rDNA gene are provided for I. andamanus and I. elegans. The Blast search results of I. andamanus showed six nucleotide differences with I. longicaudatus (FJ040495) from The Netherlands. Molecular analysis of I. elegans set this population apart from other species of Ironus, although I. elegans is closer to an undescribed Ironus (FJ040496) from The Netherlands. These are the first data of 18S rDNA for I. andamanus and I. elegans. Phylogenetic relationships with other related species of Ironus and closely related genera available in GenBank are discussed.

Studies on Ironus Bastian, 1865 (Enoplida: Ironidae) from Iran with a comment on its phylogenetic position

in Nematology



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  • View in gallery

    Ironus andamanus Sukul, 1986. A: Neck; B: Anterior end; C, D: Lip region; E: Female reproductive system; F, I: Female posterior end; G: Vagina; H: Rectum; J: Entire female.

  • View in gallery

    Ironus andamanus Sukul, 1986 (LM). A: Neck (arrow = tooth-like expansion); B: Anterior end (arrow = tooth-like expansion); C: Female posterior end. Ironus elegans Colomba & Vinciguerra, 1979 (LM). D: Neck; E: Anterior end; F: Amphid; G: Spicule and gubernaculum; H: Female posterior end; I: Male posterior end.

  • View in gallery

    Ironus elegans Colomba & Vinciguerra, 1979. A: Neck; B: Anterior end of female; C: Anterior end of male; D: Female reproductive system; E: Entire female; F: Entire male; G: Vagina; H: Female posterior end; I: Male posterior end; J: Male posterior end with lateral line; K: Gubernaculum; L: Spicules.

  • View in gallery

    Ironus ignavus Bastian, 1865. A: Neck; B: Anterior end; C: Female reproductive system; D, F: Female posterior end; E: Entire female; G: Vagina.

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    Bayesian tree inferred from 18S rDNA sequences in the order Enoplida including Ironus andamanus and I. elegans from Iran and sequences of closely related species (NCBI GenBank). Black squares indicate new sequences.

  • View in gallery

    Bayesian tree inferred from 18S rDNA sequences in the suborder Ironina including Ironus andamanus and I. elegans from Iran and sequences of closely related species (NCBI GenBank). Black squares indicate new sequences.


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