Bursaphelenchus gillanii sp. n. (Nematoda: Aphelenchoididae) – a new species of the xylophilus group in packaging wood imported from China

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A new Bursaphelenchus species of the xylophilus group was detected in coniferous packaging wood imported with goods from China in 2011. The new species is described herein and compared with other species of the xylophilus group. Bursaphelenchus gillanii sp. n. has a slim body (a = 31 (28-34) and 33 (29-36) in females and males, respectively), c′ = 3.8 (3.2-4.5) and 2.1 (1.7-2.5) in females and males, respectively, a large vulval flap, a 5-7 μm long digitate mucro as a continuation of the female tail, excretory pore at or closely posterior to the median bulb, strongly arcuate spicules, 34 (31-37) μm long as measured along the median line, with prominent pointed rostrum and small cucullus. The ITS-RFLP pattern of the new species obtained by digestion of the PCR product with RsaI, HaeIII, MspI, HinfI and AluI is different from other known Bursaphelenchus species. Results of sequencing the ITS1/2 region demonstrate the close relationship of the new species to B. mucronatus and B. xylophilus.

Nematology

International Journal of Fundamental and Applied Nematological Research

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References

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Figures

  • Bursaphelenchus gillanii sp. n. A: Female; B: Male; C: Lateral field; D: Vulval region; E: Female anterior end; F: Female tail; G: Male tail, ventral view; H: Male tail, dorsal view; I: Spicules. (Scale bars: A, B = 50 μm; C-I = 20 μm.)

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  • Light micrographs of Bursaphelenchus gillanii sp. n. Female, A-E. A: Anterior end; B: Pharynx; C: Lateral field; D: Vulva; E: Tail. Male, F-H. F: Tail papillae; G, H: Spicules. (Scale bars = 10 μm.)

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  • ITS-RFLP patterns of Bursaphelenchus gillanii sp. n. and similar species of the xylophilus group. Restriction fragments were obtained by digestion of the amplified rDNA fragment (0) with RsaI (1), HaeIII (2), MspI (3), HinfI (4) and AluI (5). M = Gene Ruler 100 bp Plus DNA Ladder.

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  • Phylogenetic relationships of Bursaphelenchus gillanii sp. n. using NJ tree construction method of aligned sequences of ITS1/2. EMBL accession numbers are listed with the species names. Numbers at branching points are bootstrap values obtained using 1000 repetitions. (Scale bar = substitutions/site.)

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  • Phylogenetic relationships of Bursaphelenchus gillanii sp. n. using UPGMA tree construction method of aligned sequences of ITS1/2. EMBL accession numbers are listed with the species names. Numbers at branching points are bootstrap values obtained using 1000 repetitions.

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