Phasmarhabditis hermaphrodita (Nematoda: Rhabditidae), a potential biocontrol agent isolated for the first time from invasive slugs in North America
In: NematologySearch for other papers by Irma Tandingan De Ley in
Current site
Google Scholar
Search for other papers by Rory D. McDonnell in
Current site
Google Scholar
Search for other papers by Sandy Lopez in
Current site
Google Scholar
Search for other papers by Timothy D. Paine in
Current site
Google Scholar
Search for other papers by Paul De Ley in
Current site
Google Scholar
Phasmarhabditis hermaphrodita is reported for the first time in North America from cadavers of the invasive slug species Deroceras reticulatum, D. laeve and Lehmannia valentiana collected from three different locations in California, USA. Four isolates were identified using combined morphology, morphometrics and molecular sequence data for complete internal transcribed spacer (ITS-1, 5.8S, ITS-2), D2-D3 expansion segments of the large subunit (LSU or 28S) and nearly complete small subunit (SSU or 18S) ribosomal DNA. Extremely low sequence variations in the COI gene of the mitochondria were observed among US isolates as well as between US isolates and the two UK sequences. The occurrence of P. hermaphrodita in North America has regulatory implications for potential biological control strategies against non-native gastropod species that are pests in ornamental and agricultural cultivation on this continent. The D2-D3 sequence of the LSU rDNA is new for the species.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Allgén C. (1950). Westschwedische marine litorale und terrestrische Nematoden. Arkiv för Zoologi 1, 301-344.
-
Andrássy I. (1976). Evolution as a basis for the systematization of nematodes. London, UK, Pitman Publishing.
-
Andrássy I. (1983). A taxonomic review of the suborder Rhabditina (Nematoda: Secernentia). Paris, France, ORSTOM.
-
Azzam K.M. (2003). Description of the nematode Phasmarhabditis tawfiki n. sp. isolated from Egyptian terrestrial snails and slugs. Journal of the Egyptian German Society of Zoology 42, 79-87.
-
Baird S.E. (2003). Strain-specific variation in the pattern of caudal papillae in Caenorhabditis briggsae (Nematoda: Rhabditidae); implications for species identification. Nematology 3, 373-376.
-
Blaxter M.L., De Ley P., Garey J.R., Liu L.X., Scheldeman P., Vierstraete A., Vanfleteren J.R., Mackey L.Y., Dorris M., Frisse L.M. et al. (1998). A molecular evolutionary framework for the phylum Nematoda. Nature 392, 71-75.
-
Bonaparte C.L.J.L. (1838). Iconographia della fauna italica per le quattro classi degli animali vertebrati. Tomo II. Amphibi. Fascicolo 23. Rome, Italy, Salviucci.
-
Brown A.P., Barker A., Hopkins A., Nelson D. (2011). Application of Phasmarhabditis hermaphrodita (Nemaslug®) to commercial broad acre crops. Proceedings of the IOPC/WPRS Working Group “Insect pathogens and entomopathogenic nematodes, Subgroup Slugs and Snails”, Bern, Switzerland, 2-4 April, 2007 and Wales, UK, 23-25 March 2010. IOBC/WPRS Bulletin 64, 99-104.
-
De Grisse A.T. (1969). Redescription ou modifications de quelques techniques utilisées dans l’étude des nématodes phytoparasitaires. Mededelingen Rijksfakulteit Landbouwwetenschappen Gent 34, 351-369.
-
De Ley P., Bert W. (2002). Video capture and editing as a tool for the storage, distribution, and illustration of morphological characters of nematodes. Journal of Nematology 34, 269-302.
-
De Ley P., Mundo-Ocampo M. (2004). The cultivation of nematodes. In: Chen Z.X., Chen S.Y., Dickson D.W. (Eds). Nematology: advances and perspectives, Vol. 1. Tsinghua, China, Tsinghua University Press, pp. 541-619.
-
De Ley P., Tandingan De Ley I.T., Morris K., Abebe E., Mundo-Ocampo M., Yoder M., Heras J., Waumann D., Rocha-Olivares A., Burr A.H.J. et al. (2005). An integrated approach to fast and informative morphological vouchering of nematodes for applications in molecular barcoding. Philosophical Transactions of the Royal Society of London B, Biological Science 360, 1945-1958.
-
Dougherty E.C. (1953). The genera of the subfamily Rhabditinae Micoletzky, 1922 (Nematoda). Thapar Commemoration 1953, pp. 69-76.
-
Dougherty E.C. (1955). The genera and species of the subfamily Rhabditinae Micoletzky, 1922 (Nematoda): a nomenclatorial analysis – including an addendum on the composition of the family Rhabditidae Örley, 1880. Journal of Helminthology 29, 105-152.
-
France A., Gerding M. (2000). Discovery of Phasmarhabditis hermaphrodita in Chile and its pathological differences with the U.K. isolate in slug control. Journal of Nematology 32, 430.
-
Genena M.A.M., Mostafa F.A.M., Fouly A.H., Yousef A.A. (2011). First record for the slug parasitic nematode, Phasmarhabditis hermaphrodita (Schneider) in Egypt. Archives of Phytopathology and Plant Protection 44, 340-345.
-
Glen D.M., Wilson M.J., Hughes L., Cargeeg P., Hajjar A. (1996). Exploring and exploiting the potential of the rhabditid nematode Phasmarhabditis hermaphrodita as a biocontrol agent for slugs. In: Henderson, I.F. (Ed.). Slugs and snails: agricultural, veterinary and environmental perspectives. BCPC Symposium Proceedings No. 66. Alton, UK, British Crop Protection Council, pp. 271-280.
-
Grewal P.S., Grewal S.K., Tan L., Adams B.J. (2002). Parasitism of molluscs by nematodes: types of associations and evolutionary trends. Journal of Nematology 35, 146-156.
-
Grewal S.K., Grewal P.S., Brown I., Tan L., Hammond R.B., Gaugler R. (2000). First North American survey for the recovery of nematodes associated with molluscs. Journal of Nematology 32, 432.
-
Hooper D.J., Wilson M.J., Rowe J.A., Glen D.M. (1999). Some observations on the morphology and protein profiles of the slug-parasitic nematodes, Phasmarhabditis hermaphrodita and P. neopapillosa (Nematoda: Rhabditidae). Nematology 1, 173-182.
-
Kanzaki N., Futai K. (2002). A PCR primer set for determination of phylogenetic relationships of Bursaphelenchus species within the xylophilus group. Nematology 4, 35-41.
-
Karimi J., Kharazi-Pakadel A., Robert S.J. (2003). Report of pathogenic nematodes of slugs, Phasmarhabditis hermaphrodita (Nematoda: Rhabditida) in Iran. Journal of Entomological Society of Iran 22, 77-78.
-
Kaya H.K., Mitani D.R. (2000). Molluscicidal nematodes for the biological control of pest slugs. Slosson Report 1999-2000, 1-4.
-
Kimball S., Mattis P., Neumann S., Natterer M. (2012). GNU Image Manipulation Program (GIMP) v 2.8. Available online at http://download.gimp.org/pub/gimp/v2.8/.
-
Maupas E. (1899). La mue et l’enkystement chez les nématodes. Archive Zoologique Expérimentelle et Générale 7, 563-628.
-
Maupas E. (1900). Modes et formes de reproduction des nématodes. Archives de Zoologique Expérimentale et Générale 8, 463-624.
-
McDonnell R.J., Paine T.D., Gormally M. (2007a). Trail-following behaviour in the malacophagous larvae of the aquatic sciomyzid flies Sepedon spinipes spinipes and Dictya montana. Journal of Insect Behavior 20, 367-376.
-
McDonnell R.J., Paine T.D., Orth R.E., Gormally M. (2007b). Life history and biocontrol potential of Dictya montana Steyskal, 1954 (Diptera: Sciomyzidae), a snail-killing fly. Pan-Pacific Entomologist 83, 101-109.
-
McDonnell R.J., Paine T.D., Gormally M. (2009). Slugs. A guide to the invasive and native fauna of California. University of California Division of Agriculture and Natural Resources Publication No. 8336.
-
Nadler S.A., D’Amelio S., Dailey M.D., Paggi L., Siu S., Sakanari J.A. (2005). Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from Northern Pacific marine mammals. Journal of Parasitology 91, 1413-1429.
-
Osche G. (1952). Systematik und Phylogenie der Gattung Rhabditis (Nematoda). Zoologische Jahrbücher (Systematik) 81, 190-280.
-
Rae R., Verdun C., Grewal P.S., Robertson J.F., Wilson M.J. (2007). Biological control of terrestrial molluscs using Phasmarhabditis hermaphrodita – progress and prospects. Pest Management Science 63, 1153-1164.
-
Ross J.L., Ivanova E.S., Severns P.M., Wilson M.J. (2010). The role of parasite release in invasion of the USA by European slugs. Biological Invasions 12, 603-610.
-
Ross J.L., Ivanova E.S., Sirgel W.F., Malan A.P., Wilson M.J. (2012). Diversity and distribution of nematodes associated with terrestrial slugs in the Western Cape Province of South Africa. Journal of Helminthology 86, 215-221.
-
Sánchez-Herráiz M.J., Barbadillo L.J., Machordom A., Sánchez B. (2000). A new species of pelodytid frog from the Iberian Peninsula. Herpetologica 56, 105-118.
-
Schneider A. (1859). Ueber eine Nematodenlarve und gewisse Verschiedenheiten in den Geschlechtsorganen der Nematoden. Zeitschrift für wissenschaftliche Zoologie 10, 176-178.
-
Schneider A. (1866). Monographie der nematoden. Berlin, Germany, Verlag von Georg Reimer.
-
Seinhorst J.W. (1959). A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4, 67-69.
-
Sudhaus W. (2011). Phylogenetic systematisation and catalogue of paraphyletic “Rhabditidae” (Secernentea, Nematoda). Journal of Nematode Morphology and Systematics 14, 113-178.
-
Tandingan De Ley I., De Ley P., Vierstraete A., Karssen G., Moens M., Vanfleteren J. (2002). Phylogenetic analyses of Meloidogyne small subunit rDNA. Journal of Nematology 34, 319-327.
-
Tandingan De Ley I., Mundo-Ocampo M., Yoder M., De Ley P. (2007). Nematodes from vernal pools in the Santa Rosa Plateau Ecological Reserve, California I. Hirschmanniella santarosae sp. n. (Nematoda: Pratylenchidae), a cryptic sibling species of H. pomponiensis Abdel-Rahman & Maggenti, 1987. Nematology 9, 405-429.
-
Ware R.W., Clark D., Crossland K., Russell R.L. (1975). The nerve ring of the nematode Caenorhabditis elegans: sensory input and motor output. Journal of Comparative Neurology 162, 71-110.
-
Wilson M.J., Glen D.M., George S.K., Butler R.C. (1993). Mass cultivation and storage of the rhabditid nematode Phasmarhabditis hermaphrodita, a biocontrol agent of slugs. Biocontrol Science and Technology 3, 513-521.
-
Wilson M.J., Glen D.M., Pearce J.D., Rodgers P.B. (1995). Monoxenic culture of the slug parasite Phasmarhabditis hermaphrodita (Nematoda: Rhabditidae) with different bacteria in liquid and solid phase. Fundamental and Applied Nematology 18, 159-166.
-
Wilson M.J., Burch G., Tourna M., Aalders L.T., Barker G.M. (2012). The potential of a New Zealand strain of Phasmarhabditis hermaphrodita for biological control of slugs. New Zealand Plant Protection 65, 161-165.
-
Yoder M., Tandingan De Ley I., King I.W., Mundo-Ocampo M., Poiras L., De Ley P. (2006). DESS: a versatile solution for preserving morphology and extractable DNA of nematodes. Nematology 8, 367-376.
-
Zaborski E.R., Gittenger L.A.S., Roberts S.J. (2001). A possible Phasmarhabditis spp. (Nematoda: Rhabditidae) isolated from Lumbricus terrestris (Oligochaeta: Lumbricidae). Journal of Invertebrate Pathology 77, 282-287.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1399 | 233 | 42 |
| Full Text Views | 304 | 7 | 0 |
| PDF Views & Downloads | 125 | 17 | 0 |
Phasmarhabditis hermaphrodita (Nematoda: Rhabditidae), a potential biocontrol agent isolated for the first time from invasive slugs in North America
In: NematologySearch for other papers by Irma Tandingan De Ley in
Current site
Google Scholar
PubMed
Search for other papers by Rory D. McDonnell in
Current site
Google Scholar
PubMed
Search for other papers by Sandy Lopez in
Current site
Google Scholar
PubMed
Search for other papers by Timothy D. Paine in
Current site
Google Scholar
PubMed
Search for other papers by Paul De Ley in
Current site
Google Scholar
PubMed
Phasmarhabditis hermaphrodita is reported for the first time in North America from cadavers of the invasive slug species Deroceras reticulatum, D. laeve and Lehmannia valentiana collected from three different locations in California, USA. Four isolates were identified using combined morphology, morphometrics and molecular sequence data for complete internal transcribed spacer (ITS-1, 5.8S, ITS-2), D2-D3 expansion segments of the large subunit (LSU or 28S) and nearly complete small subunit (SSU or 18S) ribosomal DNA. Extremely low sequence variations in the COI gene of the mitochondria were observed among US isolates as well as between US isolates and the two UK sequences. The occurrence of P. hermaphrodita in North America has regulatory implications for potential biological control strategies against non-native gastropod species that are pests in ornamental and agricultural cultivation on this continent. The D2-D3 sequence of the LSU rDNA is new for the species.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1399 | 233 | 42 |
| Full Text Views | 304 | 7 | 0 |
| PDF Views & Downloads | 125 | 17 | 0 |