Teratodiplogaster variegatae n. sp. (Nematoda: Diplogastridae) isolated from the syconia of Ficus variegata Blume on Ishigaki Island, Okinawa, Japan

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Teratodiplogaster variegatae n. sp. is described and illustrated from Ficus variegata on Ishigaki Island, Okinawa, Japan. Teratodiplogaster variegatae n. sp. is characterised by its scoop-like lip, the presence of large sac-like receptaculum seminis in the female gonads, and eight pairs of male genital papillae with an arrangement of (P1, P2, P3, vs, C, (P4, P5d), (P6, P7), P8d, Ph). It is distinguished from its close relatives T. fignewmani and T. martini by its stomatal morphology, spicule and gubernaculum morphology, structure of female reproductive organs, and the characteristic star-shaped appendage on the tail tip of males and females. Molecular phylogenetic analyses based upon near-full-length SSU and D2-D3 expansion segments of LSU corroborate a monophyletic origin of the genus Teratodiplogaster.

Teratodiplogaster variegatae n. sp. (Nematoda: Diplogastridae) isolated from the syconia of Ficus variegata Blume on Ishigaki Island, Okinawa, Japan

in Nematology



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    Teratodiplogaster variegatae n. sp. A: Entire female, right lateral view; B: Entire male, left lateral view.

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    Teratodiplogaster variegatae n. sp. A: Lip region of female, right lateral view; B: Neck region of female, right lateral view; C: Anterior gonad branch of female, right lateral view; D: Vulval region of female, ventral view; E: Vulval opening, ventral view; F: Male tail, right lateral view (d = dorsal; P + number = papilla; Ph = phasmid; vs = single mid-ventral papilla); G: Spicule and gubernaculum, right lateral view; H: Female tail, right lateral view; I: Female tail, ventral view; J: Female tail tip, right lateral view; K: Female tail tip, ventral view.

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    Lip region of Teratodiplogaster variegatae n. sp. A-C: Female in different focal planes. Stomatal sections (1-7), oval-shaped extension (ext) and cephalic sensilla (cs) are indicated. This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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    Neck region of female Teratodiplogaster variegatae n. sp. A: Entire neck region; B: Anterior end in different focal plane; C: Body surface. This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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    Vulval region of Teratodiplogaster variegatae n. sp. in different focal planes. A-H: Different focal planes, ventral view. A: Vulval opening; B, C: Vagina sphincter muscle (m); D-H: Vaginal glands (vg), receptaculum seminis (rs) and large-celled structure (ls). This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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    Female tail of Teratodiplogaster variegatae n. sp. A-E: Ventral view in different focal plane; F: Right lateral view of entire tail and tip (circled). This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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    Male tail region of Teratodiplogaster variegatae n. sp. in two different focal planes. Genital papillae are marked as ‘P + number’; the spicule keel is indicated by an arrow. This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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    Molecular phylogenetic relationship in Allodiplogaster/the Parasitodiplogaster/Teratodiplogaster clade. The consensus Bayesian tree inferred from near-full-length of SSU under GTR + I + G model (lnL=4732.9414; freqA = 0.2538; freqC = 0.2107; freqG = 0.2685; freqT = 0.2671; R(a) = 1.2936; R(b) = 3.3318; R(c) = 2.9398; R(d) = 0.7444; R(e) = 8.3423; R(f) = 1; Pinvar = 0.4486; Shape = 0.7052) and D2-D3 LSU under TrN + G model (lnL=4684.6245; freqA = 0.1896; freqC = 0.2185; freqG = 0.3376; freqT = 0.2543; R(a) = 1; R(b) = 2.1338; R(c) = 1; R(d) = 1; R(e) = 6.5843; R(f) = 1; Shape = 0.3946). The accession numbers of the compared sequences are indicated in the form: SSU/D2-D3 LSU. Posterior probability values exceeding 50% are given on appropriate clades.

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