Teratodiplogaster variegatae n. sp. (Nematoda: Diplogastridae) isolated from the syconia of Ficus variegata Blume on Ishigaki Island, Okinawa, Japan

in Nematology
Restricted Access
Get Access to Full Text
Rent on DeepDyve

Have an Access Token?



Enter your access token to activate and access content online.

Please login and go to your personal user account to enter your access token.



Help

Have Institutional Access?



Access content through your institution. Any other coaching guidance?



Connect

Teratodiplogaster variegatae n. sp. is described and illustrated from Ficus variegata on Ishigaki Island, Okinawa, Japan. Teratodiplogaster variegatae n. sp. is characterised by its scoop-like lip, the presence of large sac-like receptaculum seminis in the female gonads, and eight pairs of male genital papillae with an arrangement of (P1, P2, P3, vs, C, (P4, P5d), (P6, P7), P8d, Ph). It is distinguished from its close relatives T. fignewmani and T. martini by its stomatal morphology, spicule and gubernaculum morphology, structure of female reproductive organs, and the characteristic star-shaped appendage on the tail tip of males and females. Molecular phylogenetic analyses based upon near-full-length SSU and D2-D3 expansion segments of LSU corroborate a monophyletic origin of the genus Teratodiplogaster.

Teratodiplogaster variegatae n. sp. (Nematoda: Diplogastridae) isolated from the syconia of Ficus variegata Blume on Ishigaki Island, Okinawa, Japan

in Nematology

Sections

References

BaldwinJ.G.Giblin-DavisR.M.EddlemanC.D.WilliamsD.S.VidaJ.T.ThomasW.K. (1996). Buccal capsule of Aduncospiculum halicti (Nemata: Diplogasterina): an ultrastructural and molecular phylogenetic study. Canadian Journal of Zoology 75407-423.

BartholomaeusF.DaviesK.A.YeW.KanzakiN.Giblin-DavisR.M. (2009). Schistonchus virens sp. n. (Aphelenchoididae) and Parasitodiplogaster australis sp. n. (Diplogastridae) from Ficus virens (Moraceae) in Australia. Nematology 11583-601.

CobbN.A. (1927). Note on a new nema, Aphelenchus retusus, with a proposed division of Aphelenchus into three subgenera (Notes in Helminthological Society of Washington, Oct. 16, 1926). Journal of Parasitology 1457-58.

DaviesK.A.BartholomaeusF.YeW.KanzakiN.Giblin-DavisR.M. (2010). Schistonchus (Aphelenchoididae) from Ficus (Moraceae) in Australia, with description of S. aculeata sp. n. Nematology 12935-958.

DaviesK.A.BartholomaeusF.KanzakiN.Giblin-DavisR.M. (2013a). Schistonchus molochi sp. n. and S. athertonensis sp. n. (Aphelenchoididae) from Ficus watkinsiana (Moraceae) in Australia. Nematology 15389-400.

DaviesK.A.BartholomaeusF.KanzakiN.YeW.Giblin-DavisR.M. (2013b). Three new species of Schistonchus (Aphelenchoididae) from the Ficus subgenus Sycomorus (Moraceae) in northern Australia. Nematology 15347-362.

de ManJ.G. (1912). Helminthologische Beiträge. Zoologische Jahrbücher (Systematik)Supplement 151-104.

FuchsA.G. (1937). Neue parasitische und halbparasitische Nematoden bei Borkenkäfern und einige andere Nematoden. I. Teil. Zoologische Jahrbücher Abteilung für Systematik Öekologie und Geographie der Tiere 70291-380.

Fürst von LievenA.SudhausW. (2000). Comparative and functional morphology of the buccal cavity of Diplogastrina (Nematoda) and a first outline of the phylogeny of this taxon. Journal of Zoological Systematics and Evolutionary Research 3837-63.

Giblin-DavisR.M.DaviesK.A.TaylorG.S.ThomasW.K. (2004). Entomophilic nematode models for studying biodiversity and cospeciation. In: ChenZ.X.ChenS.Y.DicksonD.W. (Eds). Nematology advances and perspectivesVol. 1. Bejing, ChinaTsinghua University Press/Wallingford, UKCABI Publishing pp.  493-540.

Giblin-DavisR.M.YeW.KanzakiN.WilliamsD.MorrisK.ThomasW.K. (2006). Stomatal ultrastructure, molecular phylogeny, and description of Parasitodiplogaster laevigata n. sp. (Nematoda: Diplogastridae), a parasite of fig wasps. Journal of Nematology 38137-149.

HarrisonR.D.RønstedN.XuL.RasplusJ.-Y.CruaudA. (2012). Evolution of fruit traits in Ficus subgenus Sycomorus (Moraceae): to what extent do frugivores determine seed dispersal mode? PLoS ONE 7e38432.

HerreE.A. (1993). Population structure and the evolution of virulence in nematode parasites of fig wasps. Science 2591442-1445.

HerreE.A. (1995). Factors affecting the evolution of virulence: nematode parasites of fig wasps as a case study. Parasitology 111179-191.

HooperD.J. (1986). Handling, fixing, staining and mounting nematodes. In: SoutheyJ.F. (Ed.). Laboratory methods for work with plant and soil nematodes. London, UKHer Majesty’s Stationery Office pp.  59-80.

HuelsenbeckJ.P.RonquistF. (2001). MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 171754-1755.

KanzakiN.FutaiK. (2002). A PCR primer set for determination of phylogenetic relationships of Bursaphelenchus species within the xylophilus group. Nematology 435-41.

KanzakiN.Giblin-DavisR.M.DaviesK.YeW.CenterB.J.ThomasW.K. (2009). Teratodiplogaster fignewmani gen. nov., sp. nov. (Nematoda: Diplogastridae) from the syconia of Ficus racemosa in Australia. Zoological Science 26569-578.

KanzakiN.Giblin-DavisR.M.HerreE.A.CenterB.J. (2010). Redescription of two Panamanian nematodes, Parasitodiplogaster citrinema Poinar & Herre, 1991 and P. popenema Poinar & Herre, 1991 (Nematoda: Diplogastrina). Nematology 1289-104.

KanzakiN.TakiH.MasuyaH.OkabeK.TanakaR.AbeF. (2011). Diversity of stag beetle-associated nematodes in Japan. Environmental Entomology 40281-288.

KanzakiN.Giblin-DavisR.M.DaviesK.A.CenterB.J. (2012a). Teratodiplogaster martini n. sp. and Parasitodiplogaster doliostoma n. sp. (Nematoda: Diplogastridae) from the syconia of Ficus species from Africa. Nematology 14529-546.

KanzakiN.TanakaR.Giblin-DavisR.M.RagsdaleE.J.NguyenC.N.LiH.-F.LanY.-C. (2012b). A preliminary survey of fig-associated nematodes in the Asian subtropics. Journal of Nematology 44470. [Abstr.]

KanzakiN.Giblin-DavisR.M.YeW.HerreE.A.CenterB.J. (2013a). Description of Parasitodiplogaster pharmaconema n. sp. and redescription of P. maxinema from Ficus maxima Mill. (Moraceae). Nematology 15957-974.

KanzakiN.TanakaR.HirookaY.MaeharaN. (2013b). Description of Diplogasteroides andrassyi n. sp., associated with Monochamus grandis and Pinaceae trees in Japan. Journal of Nematode Morphology and Systematics 1635-47.

KanzakiN.TanakaR.Giblin-DavisR.M.DaviesK. (2014a). New plant-parasitic nematode from the mostly mycophagous genus Bursaphelenchus discovered inside figs in Japan. PLoS ONE 9e99241.

KanzakiN.Giblin-DavisR.M.YeW.HerreE.A.CenterB.J. (2014b). Parasitodiplogaster species associated with Pharmacosycea figs in Panama. Nematology 16607-619.

KanzakiN.RagsdaleE.J.Giblin-DavisR.M. (2014c). Revision of the paraphyletic genus Koerneria Meyl, 1960 and resurrection of two other genera of Diplogastridae (Nematoda). Zookeys 44217-30.

KanzakiN.RagsdaleE.J.SusoyV.SommerR.J. (2014d). Leptojacobus dorci n. gen., n. sp. (Nematoda: Diplogastridae), an associate of Dorcus stag beetles (Coleoptera: Lucanidae). Journal of Nematology 4650-59.

KanzakiN.Giblin-DavisR.M.DaviesK.A. (2014e). Diversity of fig-associated nematodes as a model system for studying biogeography host switching and colonization of new habitats. Proceedings of the 6th International Congress of Nematology p. 44. [Abstr.]

KatohK.MisawaK.KumaK.MiyataT. (2002). MAFFT: A novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 303059-3066.

KikuchiT.AikawaT.OedaY.KarimN.KanzakiN. (2009). A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchus xylophilus by loop-mediated isothermal amplification (LAMP). Phytopathology 991365-1369.

KiontkeK.ManegoldA.SudhausW. (2001). Redescription of Diplogasteroides nasuensis Takaki, 1941 and D. magnus Volk, 1950 (Nematoda: Diplogastrina) associated with Scarabaeidae (Coleoptera). Nematology 3817-832.

KörnerH. (1954). Die Nematodenfauna des vergehenden Holzes und ihre Beziehungen zu den Insekten. Zoologische Jahrbücher (Systematik) 82245-353.

LargetB.SimonD.L. (1999). Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16750-759.

LloydJ.DaviesK.A. (1997). Two new species of Schistonchus (Tylenchida: Aphelenchoididae) associated with Ficus macrophylla from Australia. Fundamental and Applied Nematology 2079-86.

MeylA.H. (1960). Die fleilebenden Erd- und Süßwassernematoden (Fadenwürmer). In: BrohmerP.EhrmannP.UlmerG. (Eds). Die Tierwelt MitteleuropasVol. 1 (5a). Leipzig, GermanyQuelle & Meyer.

MinagawaN.MizukuboT. (1994). A simple procedure of transferring nematodes to glycerol for permanent mounts. Japanese Journal of Nematology 2475.

PoinarG.O.Jr (1979). Parasitodiplogaster sycophilon gen. n., sp. n. (Diplogasteridae: Nematoda), a parasite of Elisabethiella stuchenbergi Grandi (Agaonidae: Hymenoptera) in Rhodesia. Proceedings Koninklijke Nederlandse Akademie van Wetenschappen Amsterdam Series C 82375-381.

PoinarG.O.JrHerreE.A. (1991). Speciation and adaptive radiation in the fig wasp nematode, Parasitodiplogaster in Panama. Revue de Nématologie 14361-374.

PosadaD.CrandallK.A. (1998). MODELTEST: testing the model of DNA substitution. Bioinformatics 14817-818.

RahmG. (1928). Alguns nematodes parasitas e semiparasitas das plantas culturaes do Brasil. Archivos do Instituto Biologico de Defesa Agricola e Animal (Saõ Paulo) 1239-251.

SkrjabinK.I.ShikobalovaN.P.SobolevA.A.ParamonovA.A.SudarikovA.A. (1954). [ Camellanata, Rhabditata, Tylenchata, Trichocephalata and Dioctophymata and the distribution of parasitic nematodes by hosts.] Izdatel’stvo Akademii Nauk SSSR (Moskva) 41-927.

SriwatiR.JauharlinaJ.AfriyaniTakeuchiY.TanakaR.KanzakiN. (2013). Nematodes associated with syconia of Ficus racemosa. Nematological Research 4344-45. [Abstr.]

SudhausW.Fürst von LievenA. (2003). A phylogenetic classification and catalogue of the Diplogastridae (Secernentea, Nematoda). Journal of Nematode Morphology and Systematics 643-90.

TanakaR.KikuchiT.AikawaT.KanzakiN. (2012). Simple and quick methods for nematode DNA preparation. Applied Entomology and Zoology 47291-294.

WöhrM.GreeffJ.M.KanzakiN.YeW.Giblin-DavisR.M. (2014). Molecular and morphological observations of Parasitodiplogaster sycophilon Poinar, 1979 (Nematoda: Diplogastrina) associated with Ficus burkei in Africa. Nematology 16453-462.

YeW.Giblin-DavisR.M.BraaschH.MorrisK.ThomasW.K. (2007). Phylogenetic relationships among Bursaphelenchus species (Nematoda: Parasitaphelenchidae) inferred from nuclear ribosomal and mitochondrial DNA sequence data. Molecular Phylogenetics and Evolution 431185-1197.

YoderM.De LeyI.T.KingI.Mundo-OcampoM.MannJ.BlaxterM.PoirasL.De LeyP. (2006). DESS: A versatile solution for preserving morphology and extractable DNA of nematodes. Nematology 8367-376.

Figures

  • View in gallery

    Teratodiplogaster variegatae n. sp. A: Entire female, right lateral view; B: Entire male, left lateral view.

  • View in gallery

    Teratodiplogaster variegatae n. sp. A: Lip region of female, right lateral view; B: Neck region of female, right lateral view; C: Anterior gonad branch of female, right lateral view; D: Vulval region of female, ventral view; E: Vulval opening, ventral view; F: Male tail, right lateral view (d = dorsal; P + number = papilla; Ph = phasmid; vs = single mid-ventral papilla); G: Spicule and gubernaculum, right lateral view; H: Female tail, right lateral view; I: Female tail, ventral view; J: Female tail tip, right lateral view; K: Female tail tip, ventral view.

  • View in gallery

    Lip region of Teratodiplogaster variegatae n. sp. A-C: Female in different focal planes. Stomatal sections (1-7), oval-shaped extension (ext) and cephalic sensilla (cs) are indicated. This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

  • View in gallery

    Neck region of female Teratodiplogaster variegatae n. sp. A: Entire neck region; B: Anterior end in different focal plane; C: Body surface. This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

  • View in gallery

    Vulval region of Teratodiplogaster variegatae n. sp. in different focal planes. A-H: Different focal planes, ventral view. A: Vulval opening; B, C: Vagina sphincter muscle (m); D-H: Vaginal glands (vg), receptaculum seminis (rs) and large-celled structure (ls). This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

  • View in gallery

    Female tail of Teratodiplogaster variegatae n. sp. A-E: Ventral view in different focal plane; F: Right lateral view of entire tail and tip (circled). This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

  • View in gallery

    Male tail region of Teratodiplogaster variegatae n. sp. in two different focal planes. Genital papillae are marked as ‘P + number’; the spicule keel is indicated by an arrow. This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

  • View in gallery

    Molecular phylogenetic relationship in Allodiplogaster/the Parasitodiplogaster/Teratodiplogaster clade. The consensus Bayesian tree inferred from near-full-length of SSU under GTR + I + G model (lnL=4732.9414; freqA = 0.2538; freqC = 0.2107; freqG = 0.2685; freqT = 0.2671; R(a) = 1.2936; R(b) = 3.3318; R(c) = 2.9398; R(d) = 0.7444; R(e) = 8.3423; R(f) = 1; Pinvar = 0.4486; Shape = 0.7052) and D2-D3 LSU under TrN + G model (lnL=4684.6245; freqA = 0.1896; freqC = 0.2185; freqG = 0.3376; freqT = 0.2543; R(a) = 1; R(b) = 2.1338; R(c) = 1; R(d) = 1; R(e) = 6.5843; R(f) = 1; Shape = 0.3946). The accession numbers of the compared sequences are indicated in the form: SSU/D2-D3 LSU. Posterior probability values exceeding 50% are given on appropriate clades.

Index Card

Content Metrics

Content Metrics

All Time Past Year Past 30 Days
Abstract Views 13 13 6
Full Text Views 12 12 12
PDF Downloads 2 2 2
EPUB Downloads 0 0 0