Heterogeneity of the large subunit of ribosomal RNA gene sequences in a Halicephalobus gingivalis isolate

in Nematology
Restricted Access
Get Access to Full Text
Rent on DeepDyve

Have an Access Token?

Enter your access token to activate and access content online.

Please login and go to your personal user account to enter your access token.


Have Institutional Access?

Access content through your institution. Any other coaching guidance?


If the inline PDF is not rendering correctly, you can download the PDF file here.

Heterogeneity of the large subunit of ribosomal RNA gene sequences in a Halicephalobus gingivalis isolate

in Nematology


AkagamiM.ShibaharaT.YoshigaT.TanakaN.YaguchiY.KonukiT.KondoT.YamanakaT.KuboM. (2007). Granulomatous nephritis and meningoencephalomyelitis caused by Halicephalobus gingivalis in a pony gelding. The Journal of Veterinary Medical Science 691187-1190.

AndersonR.C.LinderK.E.PeregrineA.S. (1998). Halicephalobus gingivalis (Stefanski, 1954) from a fatal infection in a horse in Ontario, Canada. Parasite 5255-261.

AndrássyI. (1984). Klasse Nematoda. Stuttgart, GermanyGustav Fischer Verlag.

BishopR.GobrightE.SpoonerP.AllsoppB.SohanpalB.CollinsN. (2000). Microsequence heterogeneity and expression of the LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva. Gene 257299-305.

ChennaR.SugawaraH.KoikeT.LopezR.GibsonT.J.HigginsD.G.ThompsonJ.D. (2003). Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Research 313497-3500.

De LucaF.ReyesA.GrunderJ.KunzP.AgostinelliA.De GiorgiC.LambertiF. (2004). Characterization and sequence variation in the rDNA region of six nematode species of the genus Longidorus (Nematoda). Journal of Nematology 36147-152.

De Sant’AnaF.J.F.Ferreira JúniorJ.A.Da CostaY.L.De ResendeR.M.BarrosC.S.L. (2012). Granulomatous meningoencephalitis due to Halicephalobus gingivalis in a horse. Brazilian Journal of Veterinary Research and Animal Science 512-15.

FilesJ.G.HirshD. (1981). Ribosomal DNA of Caenorhabditis elegans. Journal of Molecular Biology 149223-240.

FonderieP.SteelH.MoensT.BertW. (2013). Experimental induction of intraspecific morphometric variability in a single population of Halicephalobus cf. gingivalis may surpass total interspecific variability. Nematology 15529-544.

GardinerC.H.KohD.S.CardellaT.A. (1981). Micronema in man: third fatal infection. The American Journal of Tropical Medicine and Hygiene 30586-589.

IwahoriH.KanzakiN.FutaiK. (2000). A simple, polymerase chain reaction-restriction fragment length polymorphism-aided diagnosis method for pine wilt disease. Forest Pathology 30157-164.

JexA.R.HuM.RoseH.A.SchneiderM.CribbT.H.GasserR.B. (2006). Molecular characterization of Thelastomatoidea (Nematoda: Oxyurida) from cockroaches in Australia. Parasitology 133123-129.

LongE.O.DawidI.B. (1980). Repeated genes in eukaryotes. Annual Review of Biochemistry 49727-764.

McCutchanT.F.LiJ.McConkeyG.A.RogersM.J.WatersA.P. (1995). The cytoplasmic ribosomal RNAs of Plasmodium spp. Parasitology Today 11134-138.

NadlerS.A.CarrenoR.A.AdamsB.J.KindeH.BaldwinJ.G.Mundo-OcampoM. (2003). Molecular phylogenetics and diagnosis of soil and clinical isolates of Halicephalobus gingivalis (Nematoda: Cephalobina: Panagrolaimoidea), an opportunistic pathogen of horses. International Journal for Parasitology 331115-1125.

OndrejkaS.L.ProcopG.W.LaiK.K.PraysonR.A. (2010). Fatal parasitic meningoencephalomyelitis caused by Halicephalobus deletrix. Archives of Pathology & Laboratory Medicine 134625-629.

ShibaharaT.TakaiH.ShimizuC.IshikawaY.KadotaK. (2002). Equine renal granuloma caused by Halicephalobus species. Veterinary Record 151672-674.

SwoffordD.L. (1998). PAUP: Phylogenetic analysis using parsimony (and other methods)version 4.0. Sunderland, MA, USASinauer Associates.

TakaiH.ShibaharaT.MurakamiT.HayashiM.KadotaK. (2005). [ Repeat occurrence of equine Halicephalobus infection at an equestrian club.] Journal of the Japan Veterinary Medical Association 58105-108.

ThompsonJ.D.GibsonT.J.PlewniakF.JeanmouginF.HigginsD.G. (1997). The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 254876-4882.

YoshiharaT.KanemuraT.HasegawaM.TomiokaY.KanetoM.KiryuK.WadaR.WatanabeO. (1985). Micronema deletrix infection in the central nervous system of a horse. Bulletin of Equine Research Institute 2230-37.


  • View in gallery

    A: Variation of partial LSU rDNA sequence of Halicephalobus gingivalis JapanHT4677 relative to other isolates. The alignment was performed using ClustalW (Chenna et al., 2003). The asterisks indicate matched nucleotides, and bars mark gaps between the sequences. The two different sequences (JapanHT4677a, JapanHT4677b) determined in the present study are underlined; B: MP tree showing the phylogenetic relationship of H. gingivalis isolated from a horse in Ishikawa Prefecture, Japan, with Halicephalobus isolates and outgroups based on partial LSU rDNA gene sequences (tree length = 920, consistency index = 0.8565, retention index = 0.7376, rescaled consistency index = 0.6317). Multiple-sequence alignment was created with 15.0 gap-opening penalties and 6.66 gap-extension penalties using ClustalX version 1.81 (Thompson et al., 1997). Phylogenetic analysis was performed using methods in PAUP 4.0 beta Version 10 (Swofford, 1998). The bootstrap values evaluated by bootstrap resampling (1000 replicates) are shown above internal nodes. Four outgroup species were selected according to Nadler et al. (2003). The two different sequences (JapanHT4677a, JapanHT4677b) that were determined in the present study are underlined. JapanMA28826 is the H. gingivalis isolate from Ibaraki, Japan (Akagami et al., 2007). Other H. gingivalis sequences were from Nadler et al. (2003): Tenn2, TN, USA; Tenn1, TN, USA; SAN100, Guelph, ON, Canada; South Pacific, CA, USA; JB128, CA, USA; JB043, Neustadt, Germany.

Index Card

Content Metrics

Content Metrics

All Time Past Year Past 30 Days
Abstract Views 7 7 4
Full Text Views 3 3 3
PDF Downloads 0 0 0
EPUB Downloads 0 0 0