A new cyst nematode, Cactodera torreyanae sp. n. (Tylenchida: Heteroderidae), parasitising romerito, Suaeda torreyana, in Texcoco, Mexico

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A new species of cyst nematode, Cactodera torreyanae sp. n., parasitising romerito plants, Suaeda torreyana (Chenopodiaceae), found in highly saline soils in Texcoco, Mexico, is described. The new species is morphologically and molecularly related to C. weissi, from which it differs in smaller fenestral diam., longer body and shorter tail lengths of second-stage juveniles (J2) and to C. rosae, from which it differs in smaller cyst size, longer body length of J2 and the presence of a smooth eggshell surface. Phylogenetic relationships within populations and species of the Punctoderinae and Cactodera are given based on the analysis of the D2-D3 expansion segments of 28S rRNA and the ITS rRNA gene sequences.

Nematology

International Journal of Fundamental and Applied Nematological Research

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References

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Figures

  • Cactodera torreyanae sp. n. Female. A: Entire body (lateral view); B: Female (lateral) on a root; C, E, F: Vulval cones with anus and vulva; D: Neck (lateral view); G: Outline of cysts; H: Outline of females.

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  • Cactodera torreyanae sp. n. Second-stage juvenile. A: Entire body; B-D: Anterior region; E: Stylet; F: Genital primordium, after eclosion; G: Genital primordium, advanced development; H-J: Tail. Male. K, L: Anterior region; M-O: Tails with spicules in different views; P: Entire body.

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  • Light microscopic photos of Cactodera torreyanae sp. n. A: Cysts; B: White females and cysts on roots; C: White females on roots; D: White female with a gelatinous sac on a root; E: Cyst on a root; F: Head embedded into a root. (Scale bars: A = 250 μm, B, C = 300 μm, D = 120 μm, E = 100 μm, F = 35 μm.) This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/15685411.

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  • Light microscopy photos of Cactodera torreyanae sp. n. A, B: Posterior ends of female showing vulval slit; C: Neck of female; D, E: Fenestrae of cysts; G-I. Vulval cones. Anus is indicated by arrow. (Scale bars: A, B = 25 μm, C = 10 μm, D-I = 20 μm.)

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  • SEM photos of Cactodera torreyanae sp. n. A: Cyst; B: Female anterior end; C: Vulval cone of a cyst with anus; D: Surface of cyst cuticle at mid-body.

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  • Light microscopy photos of Cactodera torreyanae sp. n. Second-stage juvenile. A: Anterior region; B: Tail. Male. C: Anterior region; D: Tail. (Scale bars = 5 μm.)

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  • SEM photos of Cactodera torreyanae sp. n. Second-stage juvenile. A: Face view; B: Lateral field at mid-body. Male. C: Face view; D: Tail region with spicules and lateral field.

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  • Phylogenetic relationships within populations and species of Punctoderinae sensu Krall & Krall, 1978. The 50% majority rule consensus trees from Bayesian analysis generated from two runs as inferred from the analysis of the D2-D3 of 28S rRNA gene sequences under the GTR + G + I model. PP values are given in appropriate clades. Newly sequenced samples are indicated by bold font.  – originally identified as Cactodera estonica in GenBank.

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  • Phylogenetic relationships within populations and species of Cactodera. The 50% majority rule consensus trees from Bayesian analysis generated from two runs as inferred from the analysis of the ITS rRNA gene sequences under the GTR + G + I model. PP values are given in appropriate clades. Newly sequenced samples are indicated by bold font.  – originally identified as Cactodera estonica in GenBank; ∗∗ – originally identified as Cactodera eremica in GenBank.

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