Meloidogyne luci n. sp. (Nematoda: Meloidogynidae), a root-knot nematode parasitising different crops in Brazil, Chile and Iran

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A new root-knot nematode parasitising vegetables, flowers and fruits in Brazil, Iran and Chile, is described as Meloidogyne luci n. sp. The female has an oval to squarish perineal pattern with a low to moderately high dorsal arc and without shoulders, similar to M. ethiopica. The female stylet is robust and 15-16 μm long; the distance from the dorsal pharyngeal gland orifice to the stylet base (DGO) is 3-4 μm. Males have a high, rounded head cap continuous with the body contour. The labial disc is fused with the medial lips to form an elongated lip structure. The head region is not marked by incomplete annulations. Male stylet robust, 20.8-23.0 μm long with rounded knobs; the DGO is 2.5-4.5 μm. The stylet of second-stage juveniles (J2) is 12.0-13.5 μm long and the DGO to the stylet base is 2.3-3.3 μm. The J2 tail is conoid with finely rounded terminus and is 40.0-48.5 μm long. Biochemically, the esterase phenotype L3 (Rm: 1.05, 1.10, 1.25) is unique and is the most useful character to differentiate M. luci n. sp. from all other Meloidogyne species. Reproduction is by mitotic parthenogenesis (2n = 42-46 chromosomes). In a differential host test, the population from Lavandula spica, Caxias do Sul, RS State, Brazil, reproduced on tomato cv. Rutgers, tobacco cv. NC95 and pepper cv. California Wonder. No reproduction occurred on watermelon cv. Charleston Gray, cotton cv. Deltapine 61 or peanut cv. Florunner. In Neighbour-Joining analyses of ITS and D2-D3 rRNA sequences, populations of M. luci n. sp. from Brazil, Chile and Iran clustered together and were clearly separated from other Meloidogyne spp., thus confirming that all three populations are very similar and conspecific.

Nematology

International Journal of Fundamental and Applied Nematological Research

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References

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Figures

  • Meloidogyne luci n. sp. female. A: Pharyngeal region dorsal view; B, C: Anterior end, lateral and dorsal view respectively; D, E: Perineal patterns. (Scale bars: A-E = 20 μm.)

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  • Meloidogyne luci n. sp. female. A: Scanning electron microscopy (SEM) of anterior region, face view; B: Light microscopy of anterior region; C, D: SEM of excised stylets.

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  • Perineal patterns of Meloidogyne luci n. sp. A: Scanning electron micrograph (SEM); B-F: Light micrographs (LM).

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  • Meloidogyne luci n. sp. male. A: Anterior region in lateral view; B, C: Head ends in dorsal and lateral views; D, E: Tails of males in lateral and ventral views. (Scale bars: A = 20 μm, B-E = 10 μm.)

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  • Meloidogyne luci n. sp. male. A, B: Scanning electron micrographs (SEM) of excised stylet; C, D: Light microscopy (LM) of anterior region in dorsal and lateral views; E: LM of tail in lateral view; F: SEM of lateral fields; G, H: SEM of anterior region.

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  • Meloidogyne luci n. sp. second-stage juveniles (J2). A: Scanning electron micrograph (SEM) of cephalic region; B: Light micrographs (LM) of anterior region; C: LM of tail; D: SEM of tail.

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  • Meloidogyne luci n. sp. second-stage juveniles (J2). A: Anterior region in lateral view; B, C: Head ends in dorsal and lateral views; D, E: Tails in lateral view.

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  • Esterase phenotypes of Meloidogyne luci n. sp. (EST L3), M. inornata (EST I3) and M. ethiopica (EST E3). Meloidogyne javanica (EST J3) is included as a reference.

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  • Neighbour-Joining tree showing the phylogenetic relationships of Meloidogyne luci n. sp., with other Meloidogyne spp., based on the D2-D3 fragment of 28S rRNA sequences (A) and on the ITS1-5.8S-ITS2 rRNA sequences (B). Numbers to the left of the branches are bootstrap values for 1000 replications. Accession numbers for Meloidogyne spp. are listed in Table 1.

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