Two new species of free-living marine nematodes of Adoncholaimus Filipjev, 1918 (Oncholaimida: Oncholaimidae: Adoncholaiminae) from Hokkaido, northern Japan, with a key to species and discussion of the genus

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Two new species of free-living marine nematodes, Adoncholaimus daikokuensis sp. nov. and A. pseudofervidus sp. nov., from the coastal area of northern Japan, are described and illustrated. Adoncholaimus daikokuensis sp. nov. is similar to five congeners, A. derjugini, A. punctatus, A. oxyuroides, A. squalus comb. nov. and A. filicauda comb. nov. in the absence of a gubernaculum, but differs in the absence of a ventral swelling on tail, spicule length and buccal cavity length. Adoncholaimus pseudofervidus sp. nov. is similar to A. fervidus in having large body size, short tail, similar positions of the excretory pore and nerve ring, short spicules, arrangement of subventral setae in posterior region of body in males, and a single pair of terminal pores of the Demanian system surrounded by small gland cells in females. Adoncholaimus pseudofervidus sp. nov. differs from A. fervidus in having smaller, more anteriorly located amphids, longer buccal cavity, absence of gubernaculum in the male, and a different position of the terminal pores in the female. Partial sequences of the mitochondrial cytochrome c oxidase subunit I gene (309-337 bp) of the new species are provided for identification based on the DNA barcoding method. Metoncholaimoides is proposed as a junior synonym of Adoncholaimus. The new diagnosis of Adoncholaimus and a key to species are provided. Adoncholaimus squalus comb. nov., Adoncholaimus filicauda comb. nov., and Admirandus papillatus comb. nov. are proposed.

Two new species of free-living marine nematodes of Adoncholaimus Filipjev, 1918 (Oncholaimida: Oncholaimidae: Adoncholaiminae) from Hokkaido, northern Japan, with a key to species and discussion of the genus

in Nematology

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Figures

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    Sampling localities. A: Hokkaido; B: Daikoku Island, Akkeshi; C: Makinouchi, Nemuro; D: Shiomi, Mukawa. (Scale bars: A = 100 km; B, C = 5 km; D = 1 km.)

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    Adoncholaimus daikokuensis sp. nov., left lateral views. Holotype male (ZIHU 4162) (A-D) and paratype female (ZIHU 4166) (E-H). A: Anterior region; B: Cephalic region; C: Posterior region; D: Cloacal region; E: Posterior region; F: Reproductive system with osmosium and part of main duct; G: Anterior ductus uterinus; H: Posterior ductus uterinus. (Scale bars: A, G, H = 100 μm; B, C = 20 μm; D, E = 50 μm; F = 200 μm.)

  • View in gallery

    SEM images of Adoncholaimus daikokuensis sp. nov. Paratype female (ZIHU 4174) (A, D) and paratype male (ZIHU 4171) (B, C). A: Head, anterior view; B: Head, right lateral view; C: Anterior half of tail, left lateral view; D: Cloacal region, ventral view. (Scale bars: A, B, D = 10 μm; C = 20 μm.)

  • View in gallery

    Adoncholaimus pseudofervidus sp. nov., left lateral views. Holotype male (ZIHU 4184) (A-C) and paratype female (ZIHU 4185) (D-G). A: Anterior region; B: Cephalic region; C: Posterior region; D: Posterior region; E: Reproductive system with osmosium, ductus uterinus, and part of main duct; F: Anterior ductus uterinus and uvette; G: Posterior ductus uterinus and uvette. (Scale bars: A, F, G = 100 μm; B = 20 μm; C, D = 50 μm; E = 200 μm.)

  • View in gallery

    SEM images of Adoncholaimus pseudofervidus sp. nov. Paratype male (ZIHU 4187). A: Head, anterior view; B: Head, left lateral view; C: Tail, left lateral view; D: Cloacal region, ventral view. (Scale bars: A = 10 μm; B-D = 20 μm.)

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