Nematicides are the main method for managing root-knot nematodes but they are not environmentally benign. Biological control is one alternative that has a lower or no environmental impact. This study was conducted to evaluate the efficacy of two nematicides (Fosthiazate and Dazomet), a biocontrol agent (Purpureocillium lilacinum (= Paecilomyces lilacinus) YES-2), their combination on controlling root-knot nematodes on tomato plants and their effects on the rhizosphere microbial community in long-term glasshouse experiments. The gall index and numbers of second-stage juveniles (J2) were significantly reduced by the individual treatments of Fosthiazate, Dazomet or P. lilacinum YES-2. Purpureocillium lilacinum YES-2 combined with a half dose of Fosthiazate showed the most efficient control as indicated by gall index and number of J2 compared with the control treatment in glasshouse experiments conducted over a 5-year period (2007-2011). The bacterial and fungal biodiversity in soil measured using Biolog ECO MicroPlates and denaturing gradient gel electrophoresis (DGGE) fingerprint was higher in the treatments of P. lilacinum YES-2 alone or its combination with chemicals than in the treatments of Dazomet or Fosthiazate (in 2011 only). This study suggested a synergistic effect on the control of root-knot nematode by P. lilacinum YES-2 combined with Fosthiazate, and the contribution of these two treatments to the microbial communities in the soil.
Integrated control of fruit rot and powder mildew of chilli using the biocontrol agent Pseudomonas fluorescens and a chemical fungicide.
Postharvest biological control of blue mold of apple and brown rot of sweet cherry by natural saprophytic yeasts alone or in combination with low doses of fungicides.
Effect of storage conditions on the survival of two potential biocontrol agents of nematodes, the fungi Paecilomyces lilacinus and Pochonia chlamydosporia.
Biocontrol Science Technology18613-620.
Combination of Pseudomonas fluorescens EPS288 and reduced fungicide dose for control of Penicillium rot during postharvest storage of pear.
Microbial community composition and enzyme activities in a sandy loam soil after fumigation with methyl bromide or alternative biocides.
Soil Biology & Biochemistry381243-1254.
Integrated biological and chemical control and chemical control of damping-off caused by Rhizoctonia solani using Bacillus subtilis RB14-C and flutolanil.
Journal of Bioscience and Bioengineering91173-177.
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Suppression of Fusarium wilt of radish by co-inoculation of fluorescent Pseudomonas spp. and root-colonizing fungi.
European Journal of Plant Pathology10221-31.
Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.
Applied Environmental Microbiology59695-700.
Biological control of Fusarium crown and root rot of tomato with antagonistic bacteria and integrated control when combined with the fungicide carbendazim.
Growth promotion and biological control of root-knot nematodes in micropropagated banana during the nursery stage by treatment with specific bacterial strains.
Annals of Applied Biology15241-48.
Effects of Pseudomonas aeruginosa on the diversity of culturable microfungi and nematodes associated with tomato: impact on root-knot disease and plant growth.
Soil Biology & Biochemistry351359-1368.
Effects of antagonistic fungi and plant growth-promoting rhizobacteria on growth of tomato and reproduction of the root-knot nematode, Meloidogyne incognita.
Australasian Plant Pathology3822-28.