Two new Pseudaphelenchus species were discovered from subterranean and arboreal termites from the East Asian subtropics. Pseudaphelenchus sui n. sp. isolated from Coptotermes formosanus was collected from Miyako Isl., Okinawa, Japan, and P. scheffrahni n. sp. was isolated from Nasutitermes takasagoensis collected from the Kenting National Park in Taiwan. The two new species and P. vindai, previously described from Panamanian termites, are close to each other, and are not easily distinguished morphologically, i.e., these three are almost ‘cryptic species’. However, they can be distinguished based on minor morphological differences, including shape of male bursa (clearest in P. vindai, vague in P. sui n. sp. and intermediate in P. scheffrahni n. sp.), male tail tip (P. sui n. sp. often have a small mucron but the others do not), and in the character of the female tail (the two new species have stronger ventral curvature than P. vindai, and further, P. sui n. sp. has clear annulation at distal part, while the other species do not). Molecular phylogenetic analysis based upon near-full-length sequences of the small subunit of the ribosomal RNA gene suggested that Pseudaphelenchus and Tylaphelenchus form a strongly-supported clade at the base of the family Aphelenchoididae, and that Tylaphelenchus is included in Pseudaphelenchus as an inner clade. Therefore, based on their phylogenetic status and common morphological characters, e.g., small body and spherical median bulb, a subfamily, Tylaphelenchinae n. subfam. is proposed to embrace these two genera. In addition, Ruehmaphelenchus ipidicola n. comb. (= Tylaphelenchusipidicola) is proposed.
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Pseudaphelenchusvindai n. sp. (Tylenchomorpha: Aphelenchoididae) associated with termites (Termitidae) in Barro Colorado Island, Panama.
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A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchusxylophilus by loop-mediated isothermal amplification (LAMP).
Descriptions of four new species of Criconematidae, male of Ogma terrestris (Tylenchida: Criconematoidea) and Tylaphelenchusyamani sp. n. (Aphelenchina: Aphelenchoididae).
MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.
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