Host status and phenotypic diversity of rice cultivars resistant to Meloidogyne species under glasshouse conditions

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Five pot trials were carried out under glasshouse conditions to assess the host response of some selected rice cultivars to Meloidogyne javanica, M. paranaensis and M. enterolobii, based on nematode reproductive factor (RF) and number of nematodes (g root)−1. We also determined the broad-sense heritability and variance component estimations associated with M. paranaensis and M. incognita resistance. In addition, cluster analysis of cultivars was conducted. All cultivars were rated as susceptible or highly susceptible to M. javanica and highly resistant to M. enterolobii. To M. paranaensis, only cvs IAPAR 177 and BRS Caravela were resistant. From the principal component analysis and Ward’s cluster analysis, we obtained four conspicuous clusters of cultivars according to Meloidogyne species resistance, which demonstrates the variability of the genetic material studied. Higher broad-sense heritability values for RF and nematodes (g root)−1 were obtained. Taken together, our findings suggest that improvement of resistance of rice to Meloidogyne species resistance is possible. The implications for management programmes are also discussed.


International Journal of Fundamental and Applied Nematological Research



Araújo FilhoJ.V.MachadoA.C.Z.FerrazL.C.C.B. (2010). Host status of some selected Brazilian upland rice cultivars to Meloidogyne incognita race 4 and Rotylenchulus reniformis. Nematology 12, 929-934.

BabatolaJ.O. (1980). Reaction of some rice cultivars to the root-knot nematode, Meloidogyne incognita. Nematropica 10, 5-9.

CarneiroR.M.D.G.AlmeidaM.R.A.QuénéhervéP. (2000). Enzyme phenotypes of Meloidogyne spp. populations. Nematology 2, 645-654.

CastroJ.M.C.LimaR.D.CarneiroR.M.D.G. (2003). Variabilidade isoenzimática de populações de Meloidogyne spp. provenientes de regiões brasileiras produtoras de soja. Nematologia Brasileira 27, 1-12.

Conab (2013). Acompanhamento da safra brasileira de grãos 2012/2013 – Décimo levantamento, julho de 2013, available online at (accessed 19 July 2013).

CruzC.D. (2005). Componentes de Variância. In: CruzC.D. (Ed.). Princípios de genética quantitativa. Viçosa, MG, Brazil, UFV Press, pp.  151-215.

CruzC.D.CarneiroP.C.S. (2006). Biometric models applied to genetic breeding, 2nd edition. Viçosa, MG, Brazil, UFV Press.

DiasW.P.GarciaA.SilvaJ.F.V.CarneiroG.E.S. (2010a). Nematoides em soja: identificação e controle. Circular Técnica 76, Brazil, Embrapa Soja.

DiasW.P.DiasJ.F.V.RibeiroN.R.MoitaA.W.CarneiroR.M.D.G. (2010b). Reação de genótipos de milho a Meloidogyne mayaguensis e M. ethiopica. Nematologia Brasileira 34, 98-105.

DiomandéM. (1984). Response of upland rice cultivars to Meloidogyne species. Revue de Nématologie 7, 57-63.

EllingA.A. (2013). Major emergent problems with minor Meloidogyne species. Phytopathology 103, 1092-1102.

FalconerD.S. (1981). Introduction to quantitative genetics, 2nd edition. New York, NY, USA, Longman.

FAO (Food and Agriculture Organization of the United Nations) (2014). Website:

GomesC.B.CoutoM.E.O.CarneiroR.M.D.G. (2008). Registro de ocorrência de Meloidogyne mayaguensis em goiabeira e fumo no sul do Brasil. Nematologia Brasileira 32, 244-247.

GrecoN.Di VitoM. (2009). Population dynamics and damage levels. In: PerryR.N.MoensM.StarrJ.L. (Eds). Root-knot nematodes. Wallingford, UK, CAB International, pp.  246-274.

GuimarãesC.L.StoneL.F. (2002). Arroz de terras altas em rotação com soja. Comunicado Técnico no. 44. Brazil, Embrapa.

HartmanK.M.SasserJ.N. (1985). Identification of Meloidogyne species on the basis of differential host and perineal pattern morphology. In: BarkerK.R.CarterC.C.SasserJ.N. (Eds). An advanced treatise on Meloidogyne, Vol. II: Methodology. Raleigh, NC, USA, North Carolina State University Graphics, pp.  68-69.

HusseyR.S.BarkerK.R. (1972). A comparison of methods of collecting inocula for Meloidogyne spp., including a new technique. Plant Disease Reporter 57, 1025-1028.

IbrahimI.K.A.RezkM.A.KhalilH.A.A. (1983). Resistance of some plant cultivars to root-knot nematodes, Meloidogyne spp. Nematologia Mediterranea 11, 189-192.

LevyR.M.HomechinM.SantiagoD.C.CadioliM.C.BaidaF.C. (2009). Reação de genótipos de milho ao parasitismo de Meloidogyne incognita raça 1 e a M. paranaensis. Acta Scientiarum Agronomy 31, 575-578.

MachadoA.C.Z.SilvaS.A.DorigoO.F.RiedeC.R.GarbuglioD.D. (2014). Phenotypic variability and response of Brazilian oat genotypes to different species of root-knot and root-lesion nematodes. European Journal of Plant Pathology, in press.

MoensM.PerryR.N.StarrJ.L. (2010). Meloidogyne species – a diverse group of novel and important plant parasites. In: PerryR.N.MoensM.StarrJ.L. (Eds). Root-knot nematodes. Wallingford UK, CAB International, pp.  1-13.

MoraesO.P.CastroE.M.SouzaN.R.G.PrabhuA.S.BassinelloP.Z.FonsecaJ.R. (2004). Cultivares de arroz de terras altas para o Mato Grosso. Brazil, Circular Técnica 44.

MotaF.C.AlvesG.C.S.GibandM.GomesA.C.M.M.SouzaR.R.MattosV.S.BarbosaV.H.S.BarrosoP.A.V.NicoleM.PeixotoJ.R. (2012). New sources of resistance to Meloidogyne incognita race 3 in wild cotton accessions and histological characterization of the defense mechanisms. Plant Pathology 125, 1-11.

NoeJ.P. (1985). Analysis and interpretation of data from nematological experiments. In: BarkerK.R.CarterC.C.SasserJ.N. (Eds). An advanced treatise on Meloidogyne, Vol. II: Methodology. Raleigh, NC, USA, North Carolina State University Graphics, pp.  187-196.

R Development Core Team (2012). R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria, available online at

ScottA.J.KnottM. (1974). A cluster analysis method for grouping means in the analysis of variance. Biometrics 30, 507-512.

SilvaA.R.SantosM.A.LeiteM.T. (2004). Reprodução de nematoides de galhas em cinco cultivares de arroz de terras altas. Bioscience Journal 20, 9-12.

SilvaK.C.SilvaG.S. (2009). Reação de gramíneas e leguminosas a Meloidogyne mayaguensis. Nematologia Brasileira 33, 198-200.

SilvaR.A.Gomes FilhoG.A.AlcântaraN.R.S. (2011). Reações de cultivares de arroz a Meloidogyne incognita e M. javanica. Nematologia Brasileira 35, 82-85.

StarrJ.L.CookR.BridgeJ. (2002). Resistance to plant-parasitic nematodes: history, current use and future potential. In: StarrJ.L.CookR.BridgeJ. (Eds). Plant resistance to parasitic nematodes. Wallingford, UK, CAB International, pp.  1-17.

TancredS.J.ZeppaA.G.CooperM.StringerJ.K. (1995). Heritability and patterns of inheritance of the ripening date of apples. Horticulture Science 30, 465-474.

VencovskyR. (1987). Herança quantitativa. In: ViegasG.P. (Ed.). Melhoramento e produção de milho. Campinas, Brazil, Fundação Cargill, pp.  137-214.


  • Biplot showing the patterns of relationships among 15 rice cultivars inoculated with four Meloidogyne species based on the first principal components for resistance parameters. This figure is published in colour in the online edition of this journal, which can be accessed via

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  • Dendrogram illustrating the dissimilarity among 15 rice genotypes obtained by the Ward algorithm, based on Euclidean distance, calculated from average reproduction factor, number of nematodes (g root)−1 and weight of roots values. The thick black vertical line defines significant differences within the groups. This figure is published in colour in the online edition of this journal, which can be accessed via

    View in gallery


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