Devibursaphelenchus kheirii sp. n. (Nematoda: Ektaphelenchinae) from Iran with remarks on Devibursaphelenchus Kakuliya, 1967

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Devibursaphelenchus kheirii sp. n. is described and illustrated based on morphological, morphometric and molecular data. The new species is characterised by females with 370-472 μm body length, lip region separated from the body by a shallow constriction, three lines in lateral field, stylet short, 13.5-15.0 μm long, lacking basal knobs or swellings, excretory pore posterior to metacorpus, vulval flap absent, post-uterine sac short, without sperm, rectum and anus obscure, posterior end of the body elongate-conoid with finely rounded terminus. Males of the new species are characterised by their tail ventrally curved after fixation, having two pairs of caudal papillae, spicules 9-11 μm long with an elongated condylus, with rounded tip, pointed rostrum, lacking cucullus and having small conical terminal bursa. The new species comes close to D. hunanensis, D. lini and D. wangi. Beside morphological comparisons, the molecular phylogenetic analyses based on 778 bp of partial sequences of 28S rDNA D2-D3 were performed using two Bayesian inference (BI) and maximum likelihood (ML) methods and revealed that D. kheirii sp. n. formed a clade with the remaining species of the genus and one species of Ektaphelenchoides. This is the first report on occurrence of the genus Devibursaphelenchus in Iran. Devibursaphelenchus eproctatus syn. n. is proposed as a junior synonym of D. hunanensis.

Devibursaphelenchus kheirii sp. n. (Nematoda: Ektaphelenchinae) from Iran with remarks on Devibursaphelenchus Kakuliya, 1967

in Nematology

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References

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    Devibursaphelenchus kheirii sp. n. A: Lip region (en face view); B: Male, entire body; C: Female, entire body; D, E: Pharynx (female and male, respectively); F: Part of female reproductive system; G, H: Female posterior body; I: Male posterior body (ventral view); J: Cross section of body; K, L: Male posterior body, lateral view.

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    Devibursaphelenchus kheirii sp. n. A: Female, part of pharynx; B, C: Lip region (en face view); D: Female posterior body, showing blind end of intestine; E: Female posterior body end; F: Male posterior end (ventral view) showing bursa and second pair of papillae (arrow heads); G: Position of excretory pore (arrow head); H: Part of female reproductive system; I: Male posterior body end and papillae (arrow heads); J: Details of spicules and first pair of papillae (arrow head); K: Lateral lines. (Scale bars: A, D-I = 10 μm; B, C, J, K = 5 μm.)

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    Bayesian 50% majority rule consensus tree inferred from LSU rDNA D2-D3 under the GTR + I + G model and using Poikilolaimus piniperdae as outgroup. Bayesian posterior probabilities and maximum likelihood bootstrap values more than 50% are given for appropriate clades in the shape BPP/ML BS.

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    Bayesian 50% majority rule consensus tree inferred from LSU rDNA D2-D3 under the GTR + I + G model and using Tylaphelenchus jiaae as outgroup. Posterior probabilities more than 50% are given for appropriate clades.

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