Redescription and molecular characterisation of Xiphinema barense Lamberti et al., 1986 (Nematoda: Longidoridae) from wild olive trees in southern Italy

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A population of Xiphinema barense from wild olive trees in Torre Pozzella, Brindisi province, southern Italy, is described using both morphological and molecular studies and compared with the description of the type specimens. The wild olive nematode population agrees very well with all morphometrics provided in the original description. However, detailed observations of the lumen of the tubular portion of the uterus in paratypes and specimens of the new population revealed a clear pseudo-Z-organ with small granules mixed with crystalloid bodies which were previously undetected. Photomicrographs of adult paratypes, which were lacking in the original description, and of specimens of the new population from wild olive trees are provided. The results of the phylogenetic analyses based on the sequences of the D2-D3 expansion regions of the 28S rRNA gene and ITS rRNA genes confirm the species differentiation and indicate the phylogenetic position of X. barense and its relationship with closely related species.

Nematology

International Journal of Fundamental and Applied Nematological Research

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References

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Figures

  • Light micrographs of paratypes of Xiphinema barense Lamberti, Roca, Agostinelli & Bleve-Zacheo, 1986. A: Female anterior region; B, C: Uterine differentiation showing pseudo-Z-organ with small granules located in the lumen of the tubular portion of the uterus close to the pars dilatata uteri and crystalloid bodies; D: Female tail; E: Male tail region. Abbreviations: cb = crystalloid bodies; psZ = pseudo-Z-organ. (Scale bars = 20 μm.)

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  • Light micrographs of Xiphinema barense Lamberti, Roca, Agostinelli & Bleve-Zacheo, 1986 from wild olive trees from Torre Pozzella, Brindisi, southern Italy. A, B: Female and male entire body; C: Female anterior region; D, E: Female anterior region; F, G: Female tail region; H, I: Male tail. (Scale bars: A, B = 100 μm; C-I = 20 μm.)

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  • Light micrographs of Xiphinema barense Lamberti, Roca, Agostinelli & Bleve-Zacheo, 1986 from wild olive from Torre Pozzella, Brindisi, Italy. A: pharyngeal bulb showing dorsal and ventrosublateral nuclei; B: vulval region; C-E: Details of uterus showing pseudo-Z-organ with small granules and crystalloid bodies. Abbreviations: cb = crystalloid bodies; dn = dorsal nucleus; psZ = pseudo-Z-organ; svn = subventral nucleus. (Scale bars = 20 μm.)

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  • Line drawing of the uterine structure in Xiphinema barense Lamberti, Roca, Agostinelli & Bleve-Zacheo, 1986. A: Detail of complete anterior genital branch; B: Detail of posterior genital branch showing uterine differentiation and numerous crystalloid bodies; C: Detail of pseudo-Z-organ and crystalloid bodies.

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  • The 50% majority rule consensus trees from Bayesian analysis generated from the D2-D3 of 28S rRNA gene dataset with the TVM + I + G model. Posterior probabilities more than 65% are given for appropriate clades; bootstrap values greater than 50% are given on appropriate clades in ML analysis. Newly obtained sequences are in bold letters. The key (in colour in the online version) refers to Xiphinema spp. morphospecies groups (G1, G5, G6, G7, G8). This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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  • The 50% majority rule consensus trees from Bayesian analysis generated from the ITS region dataset with GTR + I + G model. Posterior probabilities more than 65% are given for appropriate clades; bootstrap values greater than 50% are given on appropriate clades in ML analysis. Newly obtained sequences are in bold letters. The key (in colour in the online version) refers to Xiphinema spp. morphospecies groups (G1, G5, G6, G7 and G8). This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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