Rotylenchus dalikhaniensis n. sp. (Nematoda: Hoplolaimidae), a monosexual species recovered from the rhizosphere of Ruscus hyrcanus Woronow in Mazandaran province, northern Iran

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A new monosexual species of Rotylenchus is described and illustrated based on morphological, morphometric and molecular studies from northern Iran. Rotylenchus dalikhaniensis n. sp. is characterised by having a continuous, conoid lip region with four, rarely three, annuli, lateral fields regularly areolated in pharyngeal region and irregularly throughout the body except in tail region, rarely with anastomoses at mid-body, body without longitudinal striations, stylet 34-36 μm long, vulva located at 59-64%, tail with 16-19 annuli and ventral projection, and having no males in population. The differences of the new species with five known species, namely R. breviglans, R. alii, R. pakistanensis, R. echelimae and R. provincialis, are discussed. Phylogenetic analyses using 681 bp partial sequences of D2-D3 expansion region of the 28S and 383 bp ITS1-rRNA genes revealed that the new species formed a clade with some species of Helicotylenchus and Rotylenchus, respectively.

Rotylenchus dalikhaniensis n. sp. (Nematoda: Hoplolaimidae), a monosexual species recovered from the rhizosphere of Ruscus hyrcanus Woronow in Mazandaran province, northern Iran

in Nematology

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References

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Figures

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    Line drawings of Rotylenchus dalikhaniensis n. sp. A: Entire female; B, C: Pharynx and related details; D, E: Anterior end; F: Lateral fields; G-I: Variation in tail morphology.

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    Light micrographs of Rotylenchus dalikhaniensis n. sp. A, B: Details of anterior end; C: Details of pharyngeal glands; D: Part of female reproductive system showing non-functional spermatheca without sperm; E, F: Lateral fields; G: Vulva region; H-K: Tail region showing variation in tail shape, anus and position of phasmid (K: arrow). (Scale bars = 10 μm.)

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    Light micrographs of Rotylenchus dalikhaniensis n. sp. A-E: Dorsal pharyngeal overlap in fresh females mounted in water. (Scale bars = 10 μm.)

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    Scanning electron microscope photographs of Rotylenchus dalikhaniensis n. sp. A-C: Lip region showing details and amphidial openings; D: Lateral view of female anterior region showing beginning of lateral fields (arrow); E, F: Lateral field, anterior and posterior, to vulva, respectively (arrow showing anastomosis); G, H: Tail region; I: Vulval region. (Scale bars: A, B, D-I = 2 μm; C = 1 μm.)

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    The 50% majority rule consensus trees from Bayesian analysis generated from the D2-D3 of 28S-rRNA gene dataset with the GTR + I + G model. Posterior probabilities and bootstrap values greater than 50% are given on appropriate clades. The newly obtained sequence is in bold.

  • View in gallery

    The 50% majority rule consensus trees from Bayesian analysis generated from the ITS1-rRNA gene dataset with GTR + G + I model. Posterior probabilities and bootstrap values greater than 50% are given on appropriate clades. The newly obtained sequence is in bold.

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