Additional data on Iranian trichodorids (Triplonchida: Trichodoridae) and proposal for a new synonymy

in Nematology
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Molecular data on several species of trichodorids occurring in Iran are provided. Live specimens of two species, Trichodorus arasbaranensis and T. gilanensis, were collected from their type localities. One population of T. orientalis was collected from Arasbaran forests in Eastern Azarbayjan province. The species Paratrichodorus porosus, occurring in Gilan province, and one European isolate of P. pachydermus were also included in phylogenetic analysis. An Iranian population of T. minzi, representing only the second population found of this rare species, was recovered from soil samples collected from grasslands of north-western Iran and is in morphological congruence with the original description of the species. Besides morphological study, molecular phylogenetic study was performed on newly reported species using partial 28S rDNA data, and the phylogenetic relations of all sequenced species as inferred from Bayesian analysis are discussed. According to the phylogenetic tree, two newly described species, T. golestanensis and T. iranicus, formed a clade and further studies revealed they have the same morphology and molecular sequences of 28S rDNA D2-D3. Accordingly, T. golestanensis is regarded as a junior synonym of T. iranicus according to the Principle of Priority.

Additional data on Iranian trichodorids (Triplonchida: Trichodoridae) and proposal for a new synonymy

in Nematology



De GrisseA.T. (1969). Redescription ou modifications de quelques techniques utilisées dans l’étude des nématodes phytoparasitaires. Mededelingen Faculteit Landbouwwetenschappen Rijksuniversiteit Gent 34351-369.

De WaeleD.CohnE. (1992). Trichodoridae from Israel, with a description of Trichodorus minzi n. sp. Fundamental and Applied Nematology 15201-207.

DecraemerW. (1995). The family Trichodoridae: stubby root and virus vector nematodes. Dordrecht, The NetherlandsKluwer Academic Publishers.

DecraemerW.Palomares-RiusJ.E.Cantalapiedra-NavarreteC.LandaB.B.DuarteI.AlmeidaT.VovlasN.CastilloP. (2013). Seven new species of Trichodorus (Diphtherophorina, Trichodoridae) from Spain, an apparent centre of speciation. Nematology 1557-100.

EdgarR.C. (2004). MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics 5113.

HeydariR.Tanha MaafiZ.DecraemerW. (2014a). Morphological and molecular characterisation of Trichodorus golestanensis (Nematoda: Trichodoridae), a new species from Iran. European Journal of Plant Pathology 140329-340.

HeydariR.Tanha MaafiZ.OmatiF.DecraemerW. (2014b). First report of Paratrichodorus teres (Diphtherophorina, Trichodoridae) from Iran. Australasian Plant Disease Notes 9131.

HusonD.H.ScornavaccaC. (2012). Dendroscope 3: an interactive tool for rooted phylogenetic trees and networks. Systematic Biology 611061-1067.

KiontkeK.GavinN.P.RaynesY.RoehrigC.PianoF.FitchD.H.A. (2004). Caenorhabditis phylogeny predicts convergence of hermaphroditism and extensive intron loss. Proceedings of the National Academy of Sciences of the United States of America 1019003-9008.

KumariS.SubbotinS.A. (2012). Molecular characterization and diagnostics of stubby root and virus vector nematodes of the family Trichodoridae (Nematoda: Triplonchida) using ribosomal RNA genes. Plant Pathology 611021-1031.

LargetB.SimonD.L. (1999). Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16750-759.

NiknamG.MoslehiSh.SolukiV.AshrafiM. (2010). Five species of Triplonchida from East Azarbaijan and West Azarbaijan provinces. Proceedings of the 19th Iranian Plant Protection CongressVol. IIPlant Diseases and weedsTehranIran p. 613.

NunnG.B. (1992). Nematode molecular evolution. Ph.D. Dissertation University of Nottingham Nottingham.

NylanderJ.A.A. (2004). MrModeltest. Program distributed by the author. UppsalaEvolutionary Biology Centre, Uppsala University.

PedramM.PourjamE. (2014). Description of Trichodorus iranicus sp. n. (Diphtherophorina, Trichodoridae) from Iran. Zootaxa 3795431-440.

PourjamE.AsghariR.RamajiF.A.HeydariR. (2011). Some species of plant parasitic nematodes from Iran. Iranian Journal of Plant Pathology 47141-143.

RonquistF.HuelsenbeckJ.P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 191572-1574.

TamuraK.StecherG.PetersonD.FilipskiA.KumarS. (2013). MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 302725-2729.

Tanha MaafiZ.DecraemerW. (2002). Description of Trichodorus gilanensis n. sp. from a forest park in Iran and observation on Paratrichodorus tunisiensis (Siddiqi, 1963) Siddiqi, 1974 (Nematoda: Diphtherophorina). Nematology 443-54.

WhiteheadA.G.HemmingJ.R. (1965). A comparison of some quantitative methods for extracting small vermiform nematodes from soil. Annals of Applied Biology 5525-38.

ZahediE.NiknamG.DecraemerW.KaregarA. (2009). Trichodorus arasbaranensis n. sp. (Nematoda: Trichodoridae) from a natural forest in Arasbaran, north-west Iran. Nematology 11243-252.


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    Light microphotographs of Iranian population of Trichodorus minzi De Waele & Cohn, 1992. A, B: Female. A: Anterior region; B: Pharyngeal bulb; C, D: Male. C: Dorsal intestinal overlap; D: Two cervical papillae (white arrows) located anterior to excretory pore (black arrow); E: Female posterior region; F, G: Male posterior region, showing spicules and gubernaculum; H, I: Vulval region showing vagina and lateral body pores posterior to vulva (arrows). (Scale bars = 10 μm.)

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    Bayesian 50% majority rule consensus tree inferred from LSU rDNA D2-D3 sequences of 58 trichodorid and two selected outgroup taxa under the GTR + G + I model. Bayesian posterior probabilities more than 50% are given for appropriate clades. Species in bold font are sequences from the present study; the represents the Iranian origin of the species.

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