Admirandus multicavus and Adoncholaimus ussuriensis sp. n. (Nematoda: Enoplida: Oncholaimidae) from the Sea of Japan

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A description of one new, and redescription of one known, species of the subfamily Adoncholaiminae (Nematoda: Oncholaimidae) from Peter the Great Bay (Sea of Japan) are provided. Adoncholaimus ussuriensis sp. n. is characterised by relatively large body size (L = 4.3-5.8 mm in male, 4.4-6.2 mm in female), a pair of terminal pores of the Demanian system opening dorsally at 147-189 μm (2.3-3.0 abd) anterior to the anus, excretory pore located posterior to buccal cavity region, absence of a ventral swelling on tail, long spicules (190-230 μm), presence of a gubernaculum (32-40 μm), presence of a complicated sensory field in the male with a complex supplementary organ and two sets of pilose filaments (5-10 μm long) on the posterior cloacal lobe and four rows of subventral and subdorsal postcloacal sensilla. We provide a redescription of Admirandus multicavus illustrated by light and scanning electron micrographs and propose Adoncholaimus chinensis as a junior synonym of Admirandus multicavus. The diagnoses of both genera are emended. Partial sequences of the mitochondrial gene cytochrome oxidase c subunit I (COI) and D2-D3 region of the 28S rDNA for both species were obtained. A dichotomous key to Adoncholaimus species is provided.


International Journal of Fundamental and Applied Nematological Research



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  • Admirandus multicavus. Light micrographs. A: Entire female, lateral view; B: Posterior body region of female, lateral view; C: Anterior body region of female, lateral view; D: Head of female showing horseshoe-like loops around inner labial papillae and ventral pore, lateral view; E: Posterior body region of male, lateral view. (Scale bar: A = 500 μm; B, E = 50 μm; C = 100 μm; D = 20 μm.)

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  • Admirandus multicavus. SEM. A: Posterior body region of male, ventral view; B: Head of female, dorsolateral view; C: Cloacal region of male, ventral view; D: Posterior body region of female, lateral view. (Scale bars: A, D = 20 μm; B = 10 μm; C = 4 μm; E = 50 μm.)

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  • Adoncholaimus ussuriensis sp. n. A: Entire holotype male, lateral view; B: Entire female, lateral view; C: Head of holotype male, lateral view; D: Posterior body region of holotype male, lateral view; E: Terminal pore of the Demanian system, lateral view. (Scale bars: A, B = 500 μm; C, E = 50 μm; D = 200 μm.)

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  • Adoncholaimus ussuriensis sp. n. Light micrographs. A: Entire female, lateral view; B: Head of female, lateral view; C: Ventral gland cell, lateral view; D: Posterior body region of male, lateral view (cg = caudal glands; g = gubernaculum; s = spicules); E: Tail region of male, lateral view (so = supplementary organ). (Scale bars: A = 500 μm; B = 100 μm; C, E = 50 μm; D = 100 μm.)

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  • Adoncholaimus ussuriensis sp. n., terminal pores of the Demanian system (arrows). Light micrographs. A: Lateral view; B, C: Dorsal view at different optical levels. (Scale bars: A = 100 μm; B, C = 50 μm.)

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  • Adoncholaimus ussuriensis sp. n. SEM. A: Posterior body region of female, ventro-lateral view; B: Posterior body region of female, dorsal view; C: Head of male, ventro-lateral view; D: Posterior body region of male, lateral view; E: Posterior body region of male, ventral view; F, G: Cloacal region of male, ventral view. Abbreviations: b ls = bands of long setae; pc p = precloacal papillae; pc s = precloacal setae; sd s = subdorsal setae; so = supplementary organ; sv s = subventral setae. (Scale bars: A = 40 μm; B, D, E = 20 μm; C = 10 μm; F = 4 μm; G = 3 μm.)

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  • Maximum likelihood phylogenetic tree of the subfamily Adoncholaiminae based on mitochondrial COI gene sequences. Numbers above/below branches indicate branch support based on 1000 bootstrap replicates (values < 75% are not shown).

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