Molecular characterisation of, and new data on, two populations of Xiphinema ingens Luc & Dalmasso, 1964 (Nematoda: Longidoridae) from Iran

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Two populations of Xiphinema ingens were recovered and characterised based on morphological, morphometric and molecular data. Interesting morphological variation was observed on the nature of differentiation in uterus of females between both populations, i.e. one population had only a pseudo-Z-organ in the shape of globular bodies, whilst the second population had a similar pseudo-Z-organ but also had crystalloids which varied in size and number and were located near the pseudo-Z-globules or sometimes at some distance from them towards the vagina. Variation was also observed in the shape of tail of juveniles within each population as well as between two recovered populations. Both populations had the same range of morphometric data and formed a fully supported clade in both Bayesian inference (BI) and maximum likelihood (ML) methods of phylogenetic analyses using partial sequences of 28S rDNA D2-D3 and ITS1 regions. The two populations of X. ingens formed a clade with another Xiphinema species native to Iran (X. castilloi) in 28S and two species, X. macroacanthum and X. bernardi, in ITS1 trees.

Molecular characterisation of, and new data on, two populations of Xiphinema ingens Luc & Dalmasso, 1964 (Nematoda: Longidoridae) from Iran

in Nematology

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References

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Figures

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    Line drawings of Iranian populations of Xiphinema ingens Luc & Dalmasso, 1964 (all belonging to population 1, except I and J which are population 2). A: Pharynx region; B: Posterior genital branch; C-E: Anterior end, female; F, G: Entire female; H: Globular bodies of pseudo-Z-organ in population 1; I, J: Globular bodies of pseudo-Z-organ and crystalloids in population 2; K-O: Tail of juveniles from J1-J4 (K: J1, L: J2, M, N: J3, O: J4); P-R: Female tail (P, Q: the common form of tail).

  • View in gallery

    Light micrographs of Iranian population of Xiphinema ingens Luc & Dalmasso, 1964 (population 1). A, B: Details of anterior end; C: Tubular part of uterus devoid of spines; D: Globular bodies of pseudo-Z-organ; E: Vagina region; F-K: Tail of juveniles from J1-J4 (H, I: Variation in tail of J3; J, K: Variation in tail of J4); L-O: Female tail (common forms). (Scale bars = 10 μm.)

  • View in gallery

    Light micrographs of Iranian population of Xiphinema ingens Luc & Dalmasso, 1964 (population 2). A, B: Details of anterior end; C-G: Globular bodies of pseudo-Z-organ with crystalloids (arrows) inside them (C-F) and at some distance from them and towards the vagina (G); H, I: Female tail; J: Vagina region. (Scale bars = 10 μm.)

  • View in gallery

    Light micrographs of tail of juveniles of Iranian population of Xiphinema ingens Luc & Dalmasso, 1964 (population 2). A, B: J1; C, D: J2; E: J3; F, G: J4. (Scale bars = 10 μm.)

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    Bayesian tree inferred under the GTR + I + G model from LSU D2-D3 partial sequences of two Iranian populations of Xiphinema ingens Luc & Dalmasso, 1964. Bayesian posterior probability and maximum likelihood bootstrap values exceeding 50% are given on appropriate clades in the form BPP/ML BS. Newly sequenced isolates are in boldface.

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    Bayesian tree inferred under the GTR + G model from ITS1 sequences of two Iranian populations of Xiphinema ingens Luc & Dalmasso, 1964. Bayesian posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form BPP/ML BS. Newly sequenced isolates are in boldface.

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