Root-knot nematodes are the most economically important group of plant-parasitic nematodes. In the present study, functional validation using in vitro RNAi was carried out on Meloidogyne incognita with two FMRFamide-like peptide genes, flp-14 and flp-18, and a subventral pharyngeal gland specific gene, 16D10. It was found that RNAi silencing of each gene reduced the attraction of M. incognita at different time intervals both in combination and individually. Silencing of the genes reduced nematode infection by 23-30% and development as indicated by a reduction in the number of females by 26-62%. Reproduction was decreased by 27-73% and fecundity was decreased by 19-51%. In situ hybridisation revealed the expression of flp-18 in cells associated with the ventral and retro vesicular ganglia of the central nervous system. qRT-PCR supported the correlation between phenotypic effects of silencing with that of transcript quantification.
Changes in nematode antigens recognized by monoclonal antibodies during early infections of soyabeans with the cyst nematode Heterodera glycines.
FMRFamide-like immunoreactivity within the nervous-system of the nematodes Panagrellus redivivus, Caenorhabditis elegans and Heterodera glycines.
Journal of Zoology216,
Characterisation by RNAi of pioneer genes expressed in the dorsal pharyngeal gland cell of Heterodera glycines and the effects of combinatorial RNAi.
International Journal for Parasitology38,
Short interfering RNA-mediated gene silencing in Globodera pallida and Meloidogyne incognita infective stage juveniles.
International Journal for Parasitology40,
Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene.
Proceedings of the National Academy of Sciences of the United States of America103,
Defective processing of neuropeptide precursors in Caenorhabditis elegans lacking proprotein convertase 2 (KPC-2/EGL-3): mutant analysis by mass spectrometry.
Journal of Neurochemistry98,
Localisation of Globodera pallida FMRFamide-related peptide encoding genes using in situ hybridisation.
International Journal for Parasitology32,
flp gene disruption in a parasitic nematode reveals motor dysfunction and unusual neuronal sensitivity to RNA interference.
Neuropeptide signaling systems – potential drug targets for parasite and pest control.
Current Topics in Medicinal Chemistry2,
Role of a FMRFamide-like family of neuropeptides in the pharyngeal nervous system of Caenorhabditis elegans.
Journal of Neurobiology65,
Utility of host delivered RNAi of two FMRFamide like peptides, flp-14 and flp-18, for the management of root knot nematode, Meloidogyne incognita.
PLoS ONE8, e80603.
Soil less medium for culturing and multiplication of root knot and cyst nematodes for basic and applied studies.
Annals of Plant Protection Science21,
The structure of the nervous system of the nematode Caenorhabditis elegans.
Philosophical Transactions of the Royal Society, B: Biological Sciences314,