Evaluation of rhizobacterial colonisation and the ability to induce Globodera pallida hatch

in Nematology
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Three bacterial isolates, SB13 (Acinetobacter sp.), SB14 (Arthrobacter sp.) and SB15 (Bacillus sp.), were previously isolated from the rhizosphere of sugar beet (Beta vulgaris ssp. vulgaris) plants and shown to increase hatch of potato cyst nematodes in vitro. In this study, the three isolates were assayed for rhizosphere competence. Each isolate was applied to seeds at each of four concentrations (105-108 CFU ml−1) and the inoculated seeds were planted in plastic microcosms containing coarse sand. All three isolates were shown to colonise the rhizosphere, although to differing degrees, with the higher inoculation densities providing significantly better colonisation. The isolates increased sugar beet root and shoot dry weight. Isolates SB14 and SB15 were analysed for their ability to induce in vivo hatch of Globodera pallida in non-sterile soil planted with sugar beet. After 4 and 6 weeks, both isolates had induced significantly greater percentage hatch compared to controls.

Evaluation of rhizobacterial colonisation and the ability to induce Globodera pallida hatch

in Nematology



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    Microcosm designed for rhizosphere competence experiments. Once prepared, the dish is wrapped in black plastic to prevent light penetration to roots.

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    A: Root competence of the rhizobacterial isolate SB13 after inoculation with one of four inoculum densities; B: Root competence of the rhizobacterial isolate SB14 after inoculation with one of four inoculum densities; C: Root competence of the rhizobacterial isolate SB15 after inoculation with one of four inoculum densities. Any samples which share a common letter are not significantly different using the Tukey test (P>0.05).

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    The effects of rhizobacterial isolates (SB13, SB14, SB15) from the sugar beet rhizosphere 28 days after root inoculation at four inoculum densities. A: Sugar beet root dry weight; B: Shoot dry weight. Significant differences from the corresponding controls are denoted by: (P<0.05) and ∗∗ (P<0.01), following square-root transformation.

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    Percentage in vivo hatch of Globodera pallida in the presence of sugar beet. A: 28 days; B: 42 days after inoculation with hatch-inducing bacteria. The letters a and b within the graphs indicate inoculum densities used, 107 (a) and 108 (b) CFU ml−1, respectively. ‘Sugar beet’ indicates non-inoculated sugar beet plants only. ‘Control’ indicates hatch in the absence of plant and bacteria. Asterisks indicate inoculated plants resulting in significantly different (P<0.05) hatch from that of the corresponding non-inoculated sugar beet plants.


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