Two Sheraphelenchus populations were isolated from pine wood packaging from Italy and onion bulbs from South Korea at Ningbo Port, China, respectively. The former, on the basis of morphology and molecular sequencing, is described as S. parabrevigulonis n. sp., which is characterised by a stylet length of 11 μm, three lines in the lateral field, excretory pore at level with, or slightly posterior to, nerve ring, V = 93.1, and spicules with long condylus and membrane-like rostrum in a shallow triangular shape. The species from onion, also on the basis of morphology and sequence data, was identified as S. sucus, which is characterised by a stylet length of 13 μm (erroneously stated to be ca 6 μm in the type description), excretory pore at level of, or posterior to, median bulb, and V = 80.9. The phylogeny trees based on rDNA 18S, 28S D2-D3 and ITS1/2 sequences revealed that S. parabrevigulonis n. sp. and S. sucus clustered into a clear clade and grouped with Bursaphelenchus species. The study enriched the taxonomic information of Sheraphelenchus and confirmed its status as an independent genus.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
De Grisse A.T. (1969). Redescription ou modifications de quelques techniques utilisées dans l’étude de nématodes phytoparasitaires. Mededelingen Rijksfaculteit Landbouw Wetenschappen Gent 34, 315-359.
De Ley P., Félix M.A., Frisse L.M., Nadler S.A., Sternberg P.W., Thomas W.K. (1999). Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology 1, 591-612.
Fang Y., Gu J., Wang X., Li H. (2014). Description of Aphelenchoides stellatus n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Japan. Nematology 16, 621-630.
FAO (2003). Guidelines for regulating wood packaging material in international trade. International standards for phytosanitary measures (ISPM), No. 15. Rome, Italy, FAO.
Ferris V.R., Ferris J.M., Faghihi J. (1993). Variation in spacer ribosomal DNA in some cyst-forming species of plant parasitic nematodes. Fundamental and Applied Nematology 16, 177-184.
Gu J., Wang N., He J., Wang J., Chen X., Wang X. (2014). Bursaphelenchus posterovulvus sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore. Nematology 16, 403-410.
Hunt D.J. (1993). Aphelenchida, Longidoridae and Trichodoridae: their systematics and bionomics. Wallingford, UK, CABI Publishing.
Kanzaki N., Tanaka R. (2013). Sheraphelenchus sucus n. sp. (Tylenchina: Aphelenchoididae) isolated from sap flow of Quercus serrata in Japan. Nematology 15, 975-990.
Kanzaki N., Maehara N., Aikawa T., Masuya H., Giblin-Davis R.M. (2011). Description of Bursaphelenchus kiyoharai n. sp. (Tylenchina: Aphelenchoididae) with remarks on the taxonomic framework of the Parasitaphelenchinae Ruhm, 1956 and Aphelenchoidinae Fuchs, 1937. Nematology 13, 787-840.
Kishi Y. (1970). Mimemodes japonus Reitter (Coleoptera, Rhizophagidae), an egg predator of the pine bark beetle, Cryphalus fulvus Niijima (Coleoptera: Ipidae). Kontyu 38, 195-197.
Li H., Trinh P.Q., Waeyenberge L., Moens M. (2008). Bursaphelenchus chengi sp. n. (Nematoda: Parasitaphelenchidae) isolated at Nanjing, China, in packaging wood from Taiwan. Nematology 10, 335-346.
Massey C.L., Hinds T.E. (1970). Nematodes from aspen cankers in Colorado and New Mexico. Canadian Journal of Zoology 48, 97-108.
Nickle W.R. (1970). Description of Entaphelenchidae fam. n., Roveaphelenchus jonesi gen. n., sp. n., and Sheraphelenchus entomophagus gen. n., sp. n. (Nematoda: Aphelenchoidea). Proceedings of the Helminthological Society of Washington 37, 105-109.
Nout M.J.R., Bartelt R.J. (1998). Attraction of a flying nitidulid (Carpophilus humeralis) to volatiles produced by yeasts grown on sweet corn and a corn-based medium. Journal of Chemical Ecology 24, 217-1239.
Penas A.C., Metge K., Mota M., Valadas V. (2006). Bursaphelenchus antoniae sp. n. (Nematoda: Parasitaphelenchidae) associated with Hylobius sp. from Pinus pinaster in Portugal. Nematology 8, 659-669.
Posada D., Crandall K.A. (1998). MODELTEST: testing the model of DNA substitution. Bioinformatics 14, 817-818.
Ronquist F., Huelsenbeck J.P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572-1574.
Seinhorst J.W. (1959). A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4, 67-69.
Stöver B.C., Müller K.F. (2010). TreeGraph 2: combining and visualizing evidence from different phylogenetic analyses. BMC Bioinformatics 11, 7.
Vrain T.C. (1993). Restriction fragment length polymorphism separates species of the Xiphinema americanum group. Journal of Nematology 25, 361-364.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 409 | 70 | 10 |
Full Text Views | 145 | 1 | 0 |
PDF Views & Downloads | 46 | 8 | 0 |
Two Sheraphelenchus populations were isolated from pine wood packaging from Italy and onion bulbs from South Korea at Ningbo Port, China, respectively. The former, on the basis of morphology and molecular sequencing, is described as S. parabrevigulonis n. sp., which is characterised by a stylet length of 11 μm, three lines in the lateral field, excretory pore at level with, or slightly posterior to, nerve ring, V = 93.1, and spicules with long condylus and membrane-like rostrum in a shallow triangular shape. The species from onion, also on the basis of morphology and sequence data, was identified as S. sucus, which is characterised by a stylet length of 13 μm (erroneously stated to be ca 6 μm in the type description), excretory pore at level of, or posterior to, median bulb, and V = 80.9. The phylogeny trees based on rDNA 18S, 28S D2-D3 and ITS1/2 sequences revealed that S. parabrevigulonis n. sp. and S. sucus clustered into a clear clade and grouped with Bursaphelenchus species. The study enriched the taxonomic information of Sheraphelenchus and confirmed its status as an independent genus.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 409 | 70 | 10 |
Full Text Views | 145 | 1 | 0 |
PDF Views & Downloads | 46 | 8 | 0 |