Description of Sheraphelenchus parabrevigulonis n. sp. (Nematoda: Aphelenchoididae) in pine wood packaging from Italy and redescription of S. sucus in onion bulbs from South Korea, isolated at Ningbo, China

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Two Sheraphelenchus populations were isolated from pine wood packaging from Italy and onion bulbs from South Korea at Ningbo Port, China, respectively. The former, on the basis of morphology and molecular sequencing, is described as S. parabrevigulonis n. sp., which is characterised by a stylet length of 11 μm, three lines in the lateral field, excretory pore at level with, or slightly posterior to, nerve ring, V = 93.1, and spicules with long condylus and membrane-like rostrum in a shallow triangular shape. The species from onion, also on the basis of morphology and sequence data, was identified as S. sucus, which is characterised by a stylet length of 13 μm (erroneously stated to be ca 6 μm in the type description), excretory pore at level of, or posterior to, median bulb, and V = 80.9. The phylogeny trees based on rDNA 18S, 28S D2-D3 and ITS1/2 sequences revealed that S. parabrevigulonis n. sp. and S. sucus clustered into a clear clade and grouped with Bursaphelenchus species. The study enriched the taxonomic information of Sheraphelenchus and confirmed its status as an independent genus.

Description of Sheraphelenchus parabrevigulonis n. sp. (Nematoda: Aphelenchoididae) in pine wood packaging from Italy and redescription of S. sucus in onion bulbs from South Korea, isolated at Ningbo, China

in Nematology

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References

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Figures

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    Sheraphelenchus parabrevigulonis n. sp. A: Entire female; B: Entire male; C: Head region; D: Stylet; E: Posterior part of young female gonad; F: Lateral view of mature female tail; G: Lateral lines; H: Male tail; I: Ventral view of male tail. (Scale bars = 10 μm.)

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    Light photomicrographs of Sheraphelenchus parabrevigulonis n. sp. male. A: Entire male; B-E: Head region; F, G: Caudal papillae indicated by arrows; H-O: Male tail. (Scale bars = 10 μm.)

  • View in gallery

    Light photomicrographs of Sheraphelenchus parabrevigulonis n. sp. female. A: Entire female; B: Head region; C: Lateral lines; D, E: Uterus with embryonated eggs; F-L: Lateral view of female tail; M: Ventral view of vulval region. (Scale bars = 10 μm.)

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    Sheraphelenchus sucus. A: Entire female; B: Entire male; C: Head region; D: Stylet; E: Lateral lines; F: Posterior part of young female gonad; G: Lateral view of mature female tail; H: Male tail; I: Ventral view of male tail. (Scale bars = 10 μm.)

  • View in gallery

    Light photomicrographs of Sheraphelenchus sucus male. A: Entire male; B, C: Head region, secretory-excretory pore indicated by arrow; D: Lateral lines; E, F: Caudal papillae (arrows); G, H: Male tail; I-N: Spicules. (Scale bars = 10 μm.)

  • View in gallery

    Light photomicrographs of Sheraphelenchus sucus female. A: Entire mature female; B: Entire young female; C: Vulval region; D: Female tail; E, F: Uterus without eggs; G-J: Uterus with embryonated eggs. (Scale bars = 10 μm.)

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    Phylogenetic relationships of Sheraphelenchus and aphelenchid nematodes based on 18S. The 10 001st Bayesian tree inferred from 18S under GTR + I + G model (lnL=15710.5410; freqA = 0.2507; freqC = 0.1940; freqG = 0.2593; freqT = 0.2960; R(a) = 1.2691; R(b) = 2.8781; R(c) = 1.1276; R(d) = 0.8350; R(e) = 4.2440; R(f) = 1.0000; Pinvar = 0.2839; Shape = 0.5036). Aphelenchus avenae served as the outgroup species. Posterior probability values exceeding 50% are given on appropriate clades.

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    Phylogenetic relationships of Sheraphelenchus and aphelenchid nematodes based on partial 28S. The 10 001st Bayesian tree inferred from 28S D2-D3 under TrN + I + G model (lnL=14229.2148; freqA = 0.1949; freqC = 0.1680; freqG = 0.3072; freqT = 0.3299; R(a) = 1.0000; R(b) = 3.0230; R(c) = 1.0000; R(d) = 1.0000; R(e) = 4.2819; R(f) = 1.0000; Pinvar = 0.1360; Shape = 1.0480). Aphelenchus avenae served as the outgroup species. Posterior probability values exceeding 50% are given on appropriate clades.

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    Phylogenetic relationships of Sheraphelenchus and aphelenchid nematodes based on partial ITS1/2. The 10 001st Bayesian tree inferred from ITS1/2 under GTR + I + G model (lnL=24227.2969; freqA = 0.2634; freqC = 0.1887; freqG = 0.2329; freqT = 0.3150; R(a) = 1.1499; R(b) = 2.4916; R(c) = 1.3247; R(d) = 0.8831; R(e) = 3.0866; R(f) = 1.0000; Pinvar = 0.0814; Shape = 1.4797). Aphelenchus avenae served as the outgroup species. Posterior probability values exceeding 50% are given on appropriate clades.

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