Correlations among methods to estimate lipid reserves of second-stage juveniles and its relationships with infectivity and reproduction of Meloidogyne incognita

in Nematology
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We studied the correlations between the processes used to estimate the body lipid of second-stage juveniles (J2) of Meloidogyne exigua and M. incognita, and the infectivity and reproduction of the M. incognita J2. The lipids were quantified by image analysis of the area stained red with Oil Red O dye and by the dark area of the non-stained J2. The content of neutral lipids of stained J2 of M. exigua and M. incognita correlated positively to the lipid index, body mass lipid and the dark area of J2. The period of incubation of J2 decreased the neutral lipids content and correlated significantly with the reductions of infectivity and reproduction. The period of 6 days incubation of M. incognita resulted in losses of more than 50% of the neutral lipid content, representing the threshold period for infectivity on tomato, whereas, for reproduction, the threshold was 3 days incubation.


International Journal of Fundamental and Applied Nematological Research



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  • Correlation between the percentage of body lipid of second-stage juveniles of Meloidogyne exigua by analysis of images of the red stained area and the lipid scale index (A) and the body mass lipid (B), after incubation for 12 days at 28°C.

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  • Percentage of lipid stained (A) and unstained (B) area in second-stage juveniles of Meloidogyne incognita after incubation at 28°C for different periods of time. Lipid content area was determined by image analysis. Means of eight replicates are shown. Error bars represent the standard error of the means.

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  • Oil Red O stained and unstained (body dark area) of second stage juveniles (J2) of Meloidogyne incognita showing variation in lipid content of freshly hatched (0 day) and J2 incubated at 28°C for 3, 6, 9 and 12 days. This figure is published in colour in the online edition of this journal, which can be accessed via

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