We studied the correlations between the processes used to estimate the body lipid of second-stage juveniles (J2) of Meloidogyne exigua and M. incognita, and the infectivity and reproduction of the M. incognita J2. The lipids were quantified by image analysis of the area stained red with Oil Red O dye and by the dark area of the non-stained J2. The content of neutral lipids of stained J2 of M. exigua and M. incognita correlated positively to the lipid index, body mass lipid and the dark area of J2. The period of incubation of J2 decreased the neutral lipids content and correlated significantly with the reductions of infectivity and reproduction. The period of 6 days incubation of M. incognita resulted in losses of more than 50% of the neutral lipid content, representing the threshold period for infectivity on tomato, whereas, for reproduction, the threshold was 3 days incubation.
Influence of temperature and duration of storage on the lipid reserves of entomopathogenic nematodes.
Revista Colombiana de Entomología37,
Efeito do tempo e da temperatura de incubação de juvenis do segundo estádio (J2) no teor de lipídio corporal e no parasitismo de Meloidogyne javanica em soja.
A rapid field-laboratory biossay to assess the infectivity of Meloidogyne spp. second stage juveniles.
Infectividade de juvenis do segundo estádio de Meloidogyne incognita em tomateiro após privação alimentar em solo e água em diferentes condições.
The influence of soil moisture and storage time on the motility, infectivity and lipid utilization of second stage juveniles of the potato cyst nematodes Globodera rostochiensis and G. pallida.
Revue de Nématologie10,