Correlations among methods to estimate lipid reserves of second-stage juveniles and its relationships with infectivity and reproduction of Meloidogyne incognita

in Nematology
Restricted Access
Get Access to Full Text
Rent on DeepDyve

Have an Access Token?



Enter your access token to activate and access content online.

Please login and go to your personal user account to enter your access token.



Help

Have Institutional Access?



Access content through your institution. Any other coaching guidance?



Connect

We studied the correlations between the processes used to estimate the body lipid of second-stage juveniles (J2) of Meloidogyne exigua and M. incognita, and the infectivity and reproduction of the M. incognita J2. The lipids were quantified by image analysis of the area stained red with Oil Red O dye and by the dark area of the non-stained J2. The content of neutral lipids of stained J2 of M. exigua and M. incognita correlated positively to the lipid index, body mass lipid and the dark area of J2. The period of incubation of J2 decreased the neutral lipids content and correlated significantly with the reductions of infectivity and reproduction. The period of 6 days incubation of M. incognita resulted in losses of more than 50% of the neutral lipid content, representing the threshold period for infectivity on tomato, whereas, for reproduction, the threshold was 3 days incubation.

Nematology

International Journal of Fundamental and Applied Nematological Research

Sections

References

AndalóV.MoinoA.JrMaximinianoC.CamposV.P.MendonçaL.A. (2011). Influence of temperature and duration of storage on the lipid reserves of entomopathogenic nematodes. Revista Colombiana de Entomología 37, 203-209.

AndrássyI. (1956). Die Rauminhalts- und Gewichsbestmmung der Fadenwurmer (Nematoden). Acta Zoologica Academiae Scientiarum Hungaricae 2, 1-15.

BadhwarR.RahejaR.K.AhujuS.AhujuS.P. (1995). Lipid composition of the seed gall nematode, Anguina tritici. Nematologica 41, 584-591.

BergesonG.B. (1959). The influence of temperature on the survival of some species of the genus Meloidogyne, in the absence of a host. Nematologica 4, 344-354.

BritoJ.PowersT.O.MullinP.G.InserraR.N.DicksonD.W. (2004). Morphological and molecular characterization of Meloidogyne mayaguensis isolates from Florida. Journal of Nematology 36, 232-240.

CamposH.D.CamposV.P.PozzaE.A. (2006). Efeito do tempo e da temperatura de incubação de juvenis do segundo estádio (J2) no teor de lipídio corporal e no parasitismo de Meloidogyne javanica em soja. Fitopatologia Brasileira 31, 387-393.

ChristophersA.E.P.PatelM.N.BensonJ.A.SakaV.W.EvansA.A.F.WrightD.J. (1997). A rapid field-laboratory biossay to assess the infectivity of Meloidogyne spp. second stage juveniles. Nematologica 43, 117-120.

CoolenW.A.D’HerdeC.J. (1972). A method for the quantitative extraction of nematodes from plant tissue culture. Ghent, Belgium, State Agriculture Research Centre.

CrollN.A. (1972). Energy utilization of infective Ancylostoma tubaeforme larvae. Parasitology 64, 355-365.

DasS.WesemaelW.M.L.PerryR.N. (2011). Effect of temperature and time on the survival and energy reserves of juveniles of Meloidogyne spp. Agricultural Science Research Journal 1, 102-112.

FerreiraD.F. (2000). Análises estatísticas por meio do Sisvar para Windows versão 4.0. Anais da Reunião Anual da Região Brasileira da Sociedade Internacional de Biometria 45, 255-258.

FittersP.F.L.GriffinC.T. (2004). Spontaneous and induced activity of Heterorhabditis megidis infective juveniles during storage. Nematology 6, 911-917.

FittersP.F.L.MeijerE.M.J.WrightD.J.GriffinC.T. (1997). Estimation of lipid reserves in unstained living and dead nematodes by image analysis. Journal of Nematology 29, 160-167.

FreireE.S.CamposV.P.DutraM.R.RochaF.S.SilvaJ.R.C.PozzaE.A. (2007). Infectividade de juvenis do segundo estádio de Meloidogyne incognita em tomateiro após privação alimentar em solo e água em diferentes condições. Summa Phytopathologica 33, 270-274.

GrewalP.G. (1995). Nematode quality. In: The second international symposium on entomopathogenic nematodes and their symbiotic bacteria. Honolulu, Hawaii, USA, University of Hawaii, pp.  38-40.

HusseyR.S.BarkerK.R. (1973). A comparison of methods for colecting inocula of Meloidogyne spp. including a new technique. Plant Disease Reporter 57, 1025-1028.

JepsonS.B. (1983). Identification of Meloidogyne: a general assessment and a comparison of male morphology using light microscopy with a key to 24 species. Revue de Nématologie 6, 291-309.

LeeD.L.AtkinsonH.J. (1977). Physiology of nematodes. New York, NY, USA, Columbia University Press.

PatelM.N.StolinskiM.WrightD.J. (1997). Neutral lipids and the assessment of infectivity in entomopathogenic nematodes: observations on four Steinernema species. Parasitology 114, 489-496.

QiuL.BeddingR. (1999). A rapid method for the estimation of mean dry weight and lipid content of the infective juveniles of entomopathogenic nematodes using image analysis. Nematology 1, 655-660.

QiuL.BeddingR. (2000). Energy metabolism and survival of the infective juveniles of Steinernema carpocapsae under oxygen-deficient conditions. Journal of Nematology 32, 271-280.

RobinsonM.P.AtkinsonH.J.PerryR.N. (1987). The influence of soil moisture and storage time on the motility, infectivity and lipid utilization of second stage juveniles of the potato cyst nematodes Globodera rostochiensis and G. pallida. Revue de Nématologie 10, 343-348.

RochaF.S.CamposV.P.De SouzaJ.T. (2010). Variation in lipid reserves of second-stage juveniles of Meloidogyne exigua in a coffee field and its relationship with infectivity. Nematology 12, 365-371.

SeinhorstJ.W. (1967). The relationship between population increase and population density in plant parasitic nematodes. Nematologica 13, 429-450.

SelvanS.GauglerR.LewisE.E. (1993). Biochemical energy reserves of entomopathogenic nematodes. Journal of Parasitology 79, 167-172.

StoreyR.M.J. (1983). The initial neutral lipid reserves of juveniles of Globodera spp. Nematologica 29, 144-150.

Van GundyS.D.BirdA.F.WallaceH.R. (1967). Aging and starvation in juvenile of Meloidogyne javanica and Tylenchulus semipenetrans. Phytopathology 57, 559-571.

WestermanP.R.StapelM. (1992). Linear regression models describing the performance of the insect-parasitic nematodes, Heterorhabditis sp., during storage. Fundamental and Applied Nematology 15, 525-530.

Figures

  • Correlation between the percentage of body lipid of second-stage juveniles of Meloidogyne exigua by analysis of images of the red stained area and the lipid scale index (A) and the body mass lipid (B), after incubation for 12 days at 28°C.

    View in gallery
  • Percentage of lipid stained (A) and unstained (B) area in second-stage juveniles of Meloidogyne incognita after incubation at 28°C for different periods of time. Lipid content area was determined by image analysis. Means of eight replicates are shown. Error bars represent the standard error of the means.

    View in gallery
  • Oil Red O stained and unstained (body dark area) of second stage juveniles (J2) of Meloidogyne incognita showing variation in lipid content of freshly hatched (0 day) and J2 incubated at 28°C for 3, 6, 9 and 12 days. This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

    View in gallery

Information

Content Metrics

Content Metrics

All Time Past Year Past 30 Days
Abstract Views 5 5 4
Full Text Views 4 4 4
PDF Downloads 0 0 0
EPUB Downloads 0 0 0