Acrobeloides buetschlii as a potential vector for enteric pathogens

in Nematology
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The objective of the present study was to assess if Acrobeloides buetschlii, an opportunistic species common across many soils, can be employed as a suitable model for interactions between free-living soil nematodes and enteric human pathogens. Acrobeloides buetschlii was exposed to mCherry-tagged Salmonella enterica and Escherichia coli O157:H7 and its vector potential was assessed. Salmonella cells were more readily ingested by the nematodes compared to E. coli O157:H7. Adult nematodes ingested more bacteria compared to juveniles. Salmonella survived internally for at least 7 days without affecting the viability of nematodes. Bacterial ingestion by A. buetschlii did not vary for three tested Salmonella serovars but was significantly lower for E. coli O157:H7. Considering the ubiquitous nature of pathogen and vector, these findings suggest that A. buetschlii can serve as a relevant model for studying nematode-Salmonella interactions in an agricultural setting and as potential transport for food-borne pathogens from soil to crops.

Acrobeloides buetschlii as a potential vector for enteric pathogens

in Nematology

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Figures

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    Incidence of Acrobeloides buetschlii nematodes harbouring Salmonella enterica serotype Typhimurium (SL1344) determined at different time points. The data denote the mean ± SE. Different letters indicate significant difference (Tukey, P<0.05). A representative confocal microscopy image of A. buetschlii containing mCherry-tagged S. Typhimurium (labelled bacteria in central part, in red in the colour version) following co-incubation for 2 h is shown in the inset (Scale bar = 100 μm). This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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    Effect of ambient temperature on activity and bacterial ingestion by Acrobeloides buetschlii. A: Microscopic image showing active (A) and resting, non-active (R) nematodes; B: The percentage of active nematodes; C: The incidence of A. buetschlii containing Salmonella after 2 h at different temperatures. Different letters indicate significant difference (Tukey, P<0.05) in the activity of the nematodes (B) and the incidence of nematodes (C). This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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    Incidence of juveniles and adults of Acrobeloides buetschlii containing Salmonella. The data represent the mean ± SE incidence in each population. Different letters indicate significant difference (t-test, P<0.05). A = adult; J = juvenile. This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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    Ingestion of different Salmonella enterica and Escherichia coli strains by Acrobeloides buetschlii. A: Representative confocal microscopy images showing the localisation of the different bacterial strains (red fluorescence) in the alimentary tract of A. buetschlii (Scale bar = 200 μm); B: The incidence of nematodes containing each of the bacterial species. The data represent the mean ± SE. Different letters indicate significant difference (P<0.05). This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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