Acrobeloides buetschlii as a potential vector for enteric pathogens

in Nematology
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The objective of the present study was to assess if Acrobeloides buetschlii, an opportunistic species common across many soils, can be employed as a suitable model for interactions between free-living soil nematodes and enteric human pathogens. Acrobeloides buetschlii was exposed to mCherry-tagged Salmonella enterica and Escherichia coli O157:H7 and its vector potential was assessed. Salmonella cells were more readily ingested by the nematodes compared to E. coli O157:H7. Adult nematodes ingested more bacteria compared to juveniles. Salmonella survived internally for at least 7 days without affecting the viability of nematodes. Bacterial ingestion by A. buetschlii did not vary for three tested Salmonella serovars but was significantly lower for E. coli O157:H7. Considering the ubiquitous nature of pathogen and vector, these findings suggest that A. buetschlii can serve as a relevant model for studying nematode-Salmonella interactions in an agricultural setting and as potential transport for food-borne pathogens from soil to crops.

Acrobeloides buetschlii as a potential vector for enteric pathogens

in Nematology



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    Incidence of Acrobeloides buetschlii nematodes harbouring Salmonella enterica serotype Typhimurium (SL1344) determined at different time points. The data denote the mean ± SE. Different letters indicate significant difference (Tukey, P<0.05). A representative confocal microscopy image of A. buetschlii containing mCherry-tagged S. Typhimurium (labelled bacteria in central part, in red in the colour version) following co-incubation for 2 h is shown in the inset (Scale bar = 100 μm). This figure is published in colour in the online edition of this journal, which can be accessed via

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    Effect of ambient temperature on activity and bacterial ingestion by Acrobeloides buetschlii. A: Microscopic image showing active (A) and resting, non-active (R) nematodes; B: The percentage of active nematodes; C: The incidence of A. buetschlii containing Salmonella after 2 h at different temperatures. Different letters indicate significant difference (Tukey, P<0.05) in the activity of the nematodes (B) and the incidence of nematodes (C). This figure is published in colour in the online edition of this journal, which can be accessed via

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    Incidence of juveniles and adults of Acrobeloides buetschlii containing Salmonella. The data represent the mean ± SE incidence in each population. Different letters indicate significant difference (t-test, P<0.05). A = adult; J = juvenile. This figure is published in colour in the online edition of this journal, which can be accessed via

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    Ingestion of different Salmonella enterica and Escherichia coli strains by Acrobeloides buetschlii. A: Representative confocal microscopy images showing the localisation of the different bacterial strains (red fluorescence) in the alimentary tract of A. buetschlii (Scale bar = 200 μm); B: The incidence of nematodes containing each of the bacterial species. The data represent the mean ± SE. Different letters indicate significant difference (P<0.05). This figure is published in colour in the online edition of this journal, which can be accessed via

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