The objective of the present study was to assess if Acrobeloides buetschlii, an opportunistic species common across many soils, can be employed as a suitable model for interactions between free-living soil nematodes and enteric human pathogens. Acrobeloides buetschlii was exposed to mCherry-tagged Salmonella enterica and Escherichia coli O157:H7 and its vector potential was assessed. Salmonella cells were more readily ingested by the nematodes compared to E. coli O157:H7. Adult nematodes ingested more bacteria compared to juveniles. Salmonella survived internally for at least 7 days without affecting the viability of nematodes. Bacterial ingestion by A. buetschlii did not vary for three tested Salmonella serovars but was significantly lower for E. coli O157:H7. Considering the ubiquitous nature of pathogen and vector, these findings suggest that A. buetschlii can serve as a relevant model for studying nematode-Salmonella interactions in an agricultural setting and as potential transport for food-borne pathogens from soil to crops.
Interaction of a free-living soil nematode, Caenorhabditis elegans, with surrogates of foodborne pathogenic bacteria.
Journal of Food Protection661543-1549.
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Persistence of a Salmonella enterica serovar typhimurium DT12 clone in a piggery and in agricultural soil amended with Salmonella-contaminated slurry.
Applied and Environmental Microbiology672859-2862.
Relationship between Legionella pneumophila and Acanthamoeba polyphaga: physiological status and susceptibility to chemical inactivation.
Applied and Environmental Microbiology582420-2425.
Effect of irrigation regimes on persistence of Salmonella enterica serovar Newport in small experimental pots designed for plant cultivation.
Ingestion of Salmonella enterica serotype Poona by a free-living nematode, Caenorhabditis elegans, and protection against inactivation by produce sanitizers.
Applied and Environmental Microbiology694103-4110.
Attraction of a free-living nematode, Caenorhabditis elegans, to foodborne pathogenic bacteria and its potential as a vector of Salmonella Poona for preharvest contamination of cantaloupe.
Journal of Food Protection661964-1971.
Caenorhabditis elegans genomic response to soil bacteria predicts environment-specific genetic effects on life history traits.
Lethal paralysis of Caenorhabditis elegans by Pseudomonas aeruginosa.
Proceedings of the National Academy of Science of the United States of America9615202-15207.
Potential role of Diploscapter sp. strain LKC25, a bacterivorous nematode from soil, as a vector of food-borne pathogenic bacteria to preharvest fruits and vegetables.
Applied and Environmental Microbiology712433-2437.
Dynamics of nematodes and protozoa following the experimental addition of cattle or pig slurry to soil.
Soil Biology & Biochemistry301379-1387.
Persistence of enterohemorrhagic Escherichia coli O157:H7 in soil and on leaf lettuce and parsley grown in fields treated with contaminated manure composts or irrigation water.
Journal of Food Protection671365-1370.
Movement and persistence of faecal bacteria in agricultural soils and subsurface drainage water: a review.
Canadian Biosystems Engineering441.1-1.9.
Effectiveness of cleaners and sanitizers in killing Salmonella Newport in the gut of a free-living nematode, Caenorhabditis elegans.
Journal of Food Protection672151-2157.
Persistence of Escherichia coli O157:H7, Salmonella Newport, and Salmonella Poona in the gut of a free-living nematode, Caenorhabditis elegans, and transmission to progeny and uninfected nematodes.
International Journal of Food Microbiology101227-236.
Migration of Caenorhabditis elegans to manure and manure compost and potential for transport of Salmonella newport to fruits and vegetables.
International Journal of Food Microbiology10661-68.
Use of bioluminescent bacterial biosensors to investigate the role of free-living helminths as reservoirs and vectors of Salmonella.
Environmental Microbiology Reports1198-207.
Complete killing of Caenorhabditis elegans by Burkholderia pseudomallei is dependent on prolonged direct association with the viable pathogen.
Comparative proteomic analysis of extracellular proteins of enterohemorrhagic and enteropathogenic Escherichia coli strains and their ihf and ler mutants.
Applied and Environmental Microbiology705274-5282.
Enteropathogenic Escherichia coli subverts phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate upon epithelial cell infection.
Molecular Biology of the Cell20544-555.
Feeding biology of Diplogasteritus nudicapitatus and Rhabditis curvicaudata (Nematoda) related to food concentration and temperature, in sewage treatment plants.