Bio-management of tomato wilt complex caused by Meloidogyne incognita and Fusarium oxysporum f. sp. lycopersici

in Nematology
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The potential of biocontrol agents Purpureocillium lilacinum (Paecilomyces lilacinus) and Trichoderma harzianum was evaluated against tomato wilt complex, caused by a combination of Meloidogyne incognita and Fusarium oxysporum f. sp. lycopersici, under both laboratory and field conditions. Biocontrol agents at spore concentration of 1 × 106 spores ml−1 were applied alone and in combined treatments. The results of combined application revealed maximum mortality and inhibition of hatching of M. incognita under in vitro conditions. Combined application of both antagonistic fungi was found to be more effective in mycelial inhibition of Fusarium oxysporum f. sp. lycopersici. In glasshouse trials, application of T. harzianum promoted overall plant growth, followed by combined application of P. lilacinum and T. harzianum; nematode development parameters and fungus damage were significantly reduced. Under field conditions, the combined application of P. lilacinum and T. harzianum increased the number of leaves, shoot length, shoot weight and root length, and decreased root weight, with minimum number of females and egg masses of M. incognita per root system and mycelia inhibition of F. oxysporum.

Bio-management of tomato wilt complex caused by Meloidogyne incognita and Fusarium oxysporum f. sp. lycopersici

in Nematology



Al-SaeediS.S.Al-AniB.M. (2014). Study of antagonistic capability of Trichoderma harzianum isolates against some pathogenic soil-borne fungi. Agriculture and Biology Journal of North America 515-23.

AminF.RazdanV.K.MohiddinF.A.BhatK.A.BandayS. (2010). Potential of Trichoderma species as biocontrol agents of soil-borne fungal propagules. Journal of Phytology 238-41.

DatnoffL.NemecS.PerneznyK. (1995). Biological control of Fusarium crown and root rot of tomato in Florida using Trichoderma harzianum and Glomus intraradices. Biological Control 5427-431.

DennisC.WebsterJ. (1971). Antagonistic properties of species-groups of Trichoderma: III. Hyphal interaction. Transactions of the British Mycological Society 57363-369.

DesaegerJ.RaoM.R. (2000). Infection and damage potential of Meloidogyne javanica on Sesbania sesban in different soil types. Nematology 2169-178.

DjianC.PijarowskiL.PonchetM.ArpinN.Favre-BonvinJ. (1991). Acetic acid: a selective nematicidal metabolite from culture filtrates of Paecilomyces lilacinus (Thom) Samson and Trichoderma longibrachiatum Rifai. Nematologica 37101-112.

DubeyS.C.SureshM.SinghB. (2007). Evaluation of Trichoderma species against Fusarium oxysporum f. sp. ciceris for integrated management of chickpea wilt. Biological Control 40118-127.

EapenS.J.BeenaB.RamanaK. (2005). Tropical soil microflora of spice-based cropping systems as potential antagonists of root-knot nematodes. Journal of Invertebrate Pathology 88218-225.

EladY. (2000). Biological control of foliar pathogens by means of Trichoderma harzianum and potential modes of action. Crop Protection 19709-714.

EvansH.HolmesK.ThomasK. (2003). Mycobiota of an indigenous Theobroma species (Sterculiaceae) in Ecuador: assessing its potential for biological control of cocoa diseases. Mycological Progress 2149-160.

FAO (2010). FAOstat database collection.

FreemanC.EvansC.MonteithD.ReynoldsB.FennerN. (2001). Export of organic carbon from peat soils. Nature 412785.

GethaK.VikineswaryS.WongW.SekiT.WardA.GoodfellowM. (2005). Evaluation of Streptomyces sp. strain g10 for suppression of Fusarium wilt and rhizosphere colonization in pot-grown banana plantlets. Journal of Industrial Microbiology and Biotechnology 3224-32.

HolbrookC.C.KnauftD.A.DicksonD.W. (1983). A technique for screening peanut for resistance to Meloidogyne incognita. Plant Disease 57957-958.

HollandR.J.WilliamsK.L.KhanA. (1999). Infection of Meloidogyne javanica by Paecilomyces lilacinus. Nematology 1131-139.

HuangX.ZhaoN.ZhangK. (2004). Extracellular enzymes serving as virulence factors in nematophagous fungi involved in infection of the host. Research in Microbiology 155811-816.

HusseyR.BarkerK. (1973). Comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter 571025-1028.

KirankumarR.JagadeeshK.KrishnarajP.PatilM. (2010). Enhanced growth promotion of tomato and nutrient uptake by plant growth promoting rhizobacterial isolates in presence of tobacco mosaic virus pathogen. Karnataka Journal of Agricultural Sciences 21309-311.

MikamiY.YazawaK.FukushimaK.AraiT.UdagawaS.-I.SamsonR.A. (1989). Paecilotoxin production in clinical or terrestrial isolates of Paecilomyces lilacinus strains. Mycopathologia 108195-199.

PapavizasG. (1985). Trichoderma and Gliocladium: biology, ecology, and potential for biocontrol. Annual Review of Phytopathology 2323-54.

ParkJ.O.HargreavesJ.McConvilleE.StirlingG.GhisalbertiE.SivasithamparamK. (2004). Production of leucinostatins and nematicidal activity of Australian isolates of Paecilomyces lilacinus (Thom) Samson. Letters in Applied Microbiology 38271-276.

QuesenberryK.H.BaltenspergerD.D.DunnR.A.WilcoxC.J.HardyS.R. (1989). Selection for tolerance to root-knot nematodes in redclover. Crop Science 2962-65.

SinghS.MathurN. (2010). In vitro studies of antagonistic fungi against the root-knot nematode, Meloidogyne incognita. Biocontrol Science and Technology 20275-282.

Suárez-EstrellaF.Vargas-GarciaC.LopezM.CapelC.MorenoJ. (2007). Antagonistic activity of bacteria and fungi from horticultural compost against Fusarium oxysporum f. sp. melonis. Crop Protection 2646-53.

SunM.-H.GaoL.ShiY.X.LiB.J.LiuX.Z. (2006). Fungi and actinomycetes associated with Meloidogyne spp. eggs and females in China and their biocontrol potential. Journal of Invertebrate Pathology 9322-28.

TunlidA.JanssonS. (1991). Proteases and their involvement in the infection and immobilization of nematodes by the nematophagous fungus Arthrobotrys oligospora. Applied and Environmental Microbiology 572868-2872.

ZakiM.MaqboolM. (1991). Paecilomyces lilacinus controls Meloidogyne javanica on chickpea. International Chickpea Newsletter 2522-23.

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