Rhabditidoides humicolus n. sp. associated with arthropods from rotting palm tissue in Florida, USA

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Rhabditidoides humicolus n. sp. is described and illustrated from arthropods associated with decaying tissue from the crown shaft of a living spindle palm, Hyophorbe verschaffeltii, in southern Florida, USA. In addition to its generic character, i.e., the arrangement of male genital papillae, ⟨v1, v2, v3d, CO, v4, (ad, v5, ph, v6), (pd, v7)⟩, the new species is characterised by its small stomatal flaps, a secretory pore-like opening, a pair of deirids, two pairs of post-deirids and small subventral vulval papillae located just anterior to the vulva. Besides those newly found characters, there are only a few typological differences between R. humicolus n. sp. and several previously described species in the genus. However, based on the biological characters, e.g., gonochoristic reproduction, association as dauers with the crane fly, Limonia (Rhipidia) schwarzi (Diptera: Limoniidae), millipedes, an immature cockroach, and staphylinid beetle adults, and distribution in southern Florida, the new species was considered to be different from others in the genus.

Rhabditidoides humicolus n. sp. associated with arthropods from rotting palm tissue in Florida, USA

in Nematology

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References

AndrássyI. (1984). Klasse Nematoda (Ordnungen Monhysterida, Desmoscolecida, Araeolaimida, Chromadorida, Rhabditida). In: Bestimmungsbücher zur Bodenfauna Europas. Stuttgart, GermanyFischer.

de ManJ.G. (1912). Helminthologische Beiträge. Zoologische Jahrbücher JenaSupplement 15(1) 439-464.

Giblin-DavisR.M.YeW.KanzakiN.WilliamsD.MorrisK.ThomasW.K. (2006). Stomatal ultrastructure, molecular phylogeny, and description of Parasitodiplogaster laevigata n. sp. (Nematoda: Diplogastridae), a parasite of fig wasps from Ficus laevigata from Florida. Journal of Nematology 38137-149.

Giblin-DavisR.M.KanzakiN.De LeyP.WilliamsD.S.SchierenbergE.RagsdaleE.ZengY.CenterB.J. (2010). Ultrastructure and life history of Myolaimus byersi n. sp.; a phoretic associate of the crane fly Limonia schwarzi (Tipulidae) in Florida. Nematology 12519-542.

Heindl-MengertH. (1956). Die Nematodenfauna im Schleimfluß lebender Laubbäume. Sitzungsberichte der Physikalisch-medizinischen Sozietät zu Erlangen 77389-395.

HerrmannM.KienleS.MayerW.E.RochatJ.SommerR.J. (2010). Haplotype diversity of the nematode Pristionchus pacificus on Réunion in the Indian Ocean suggests multiple independent invasions. Biological Journal of the Linnean Society 100170-179.

HooperD.J. (1986). Handling, fixing, staining and mounting nematodes. In: SoutheyJ.F. (Ed.). Laboratory methods for work with plant and soil nematodes. London, UKHer Majesty’s Stationery Office pp.  59-80.

KanzakiN. (2013). Simple methods for morphological observation of nematodes. Nematological Research 439-13.

KanzakiN.Giblin-DavisR.M.ZengY.YeW.CenterB.J. (2008). Diplogastrellus metamasius n. sp. (Rhabditida: Diplogastridae) isolated from Metamasius hemipterus (Olivier) (Coleoptera: Dryophthoridae) in southern Florida and Costa Rica. Nematology 10853-868.

KanzakiN.Giblin-DavisR.M.ZengY.YeW.CenterB.J. (2009). Acrostichus rhynchophori n. sp. (Rhabditida: Diplogastridae): a phoretic associate of Rhynchophorus cruentatus Fabricius and R. palmarum L. (Coleoptera: Curculionidae) in the Americas. Nematology 11669-688.

KanzakiN.TakiH.MasuyaH.OkabeK.TanakaR.AbeF. (2011). Diversity of stag beetle-associated nematodes in Japan. Environmental Entomology 40281-288.

KanzakiN.RagsdaleE.J.HerrmannM.MayerW.E.SommerR.J. (2012). Description of three nematode species from Japan, Pristionchus exspectatus n. sp., P. arcanus n. sp., and P. japonicus n. sp., which form a cryptic species complex including the model organism P. pacificus (Diplogastridae). Zoological Science 29403-417.

KanzakiN.RagsdaleE.J.HerrmannM.RöselerW.SommerR.J. (2013a). Two new species of Pristionchus (Nematoda: Diplogastridae) from Japan, including a new member of the P. pacificus species complex. Zoological Science 30680-692.

KanzakiN.RagsdaleE.J.HerrmannM.SommerR.J.SusoyV. (2013b). Two androdioecious and one dioecious new species of Pristionchus (Nematoda: Diplogastridae): new reference points for the evolution of reproductive mode. Journal of Nematology 45172-194.

KanzakiN.TanakaR.HirookaY.MaeharaN. (2013c). Description of Diplogasteroides andrassyi n. sp., associated with Monochamus grandis and Pinaceae trees in Japan. Journal of Nematode Morphology and Systematics 1635-47.

KanzakiN.RagsdaleE.J.HerrmannM.SommerR.J. (2014). Two new and two recharacterized species from a radiation of Pristionchus (Nematoda: Diplogastridae) in Europe. Journal of Nematology 4660-74.

KiontkeK.C.FélixM.-A.AilionM.RockmanM.V.BraendleC.PénigaultJ.-B.FitchD.H.A. (2011). A phylogeny and molecular barcodes for Caenorhabditis, with numerous new species from rotting fruits. BMC Evolutionary Biology 11339.

KreisH.A. (1932). Beiträge zur Kenntnis pflanzenparasitischer Nematoden. Zeitschrift für Parasitenkunde 5184-194.

MahamoodM.AhmadI. (2009). Nematodes of the order Rhabditida from India. Description of Diplogasteroides uriceus sp. n. and Rhabditidoides papillatus sp. n. (Diplogastrina). Nematology 11541-550.

MeylA.H. (1954). Die bisher in Italien gefundenen freilebenden Erd- und Süßwasser-Nematoden. Archivio Zoologico Italiano 39161-264.

MicoletzkyH. (1922). Die freilebenden Erd-Nematoden. Archiv fur Naturgeschichte 87A(1921) 1-650.

MinagawaN.MizukuboT. (1994). A simplified procedure of transferring nematodes to glycerol for permanent mounts. Japanese Journal of Nematology 2475.

ParamonovA.A. (1952). [ Ecological classification of plant nematodes.] Trudy Gelminthologicheskoi Laboratorii Akademia Nauk SSSR (Moskva) 6338-369.

PhotosA.GutierrezA.SommerR.J. (2006). sem-4/spalt and egl-17/FGF have a conserved role in sex myoblast specification and migration in P. pacificus and C. elegans. Developmental Biology 293142-153.

PoinarG.O.Jr (1979). Parasitodiplogaster sycophilon gen. n., sp. n. (Diplogasteridae: Nematoda), a parasite of Elisabethiella stuckenbergi Grandi (Agaonidae: Hymenoptera) in Rhodesia. Proceedings of the Koninklijke Nederlandse Akademie van Wetenschappen Series C Biological and Medical Sciences 82375-381.

RagsdaleE.J.KanzakiN.RöselerW.HerrmannM.SommerR.J. (2013). Three new species of Pristionchus (Nematoda: Diplogastridae) show morphological divergence through evolutionary intermediates of a novel feeding polymorphism. Zoological Journal of the Linnean Society 168671-698.

RahmG. (1928). Alguns nematodes parasitas e semiparasitas das plantas culturaes do Brasil. Archivos do Instituto Biologico de Defesa Agricola e Animal (São Paulo) 1239-251.

Schuurmans StekhovenJ.H. (1951). Nématodes saprozoaires et libres du Congo Belge. Mémoires de l’Institut Royal des Sciences Naturelles de Belgique Série 2 393-79.

SteinerG. (1930). The nemic fauna of the slime flux of the Carolina poplar. Journal of Agricultural Research 41427-434.

SteinerG. (1943). New nematodes associated with a disease of the Papaya in Chile. Boletin Departamento de Sanidad Vegetal (Santiago) 395-116.

SudhausW.Fürst von LievenA. (2003). A phylogenetic classification and catalogue of the Diplogastridae (Secernentea, Nematoda). Journal of Nematode Morphology and Systematics 643-89.

SusoyV.KanzakiN.HerrmannM. (2013). Description of bark beetle-associated nematodes Micoletzkya masseyi n. sp. and M. japonica n. sp. (Nematoda: Diplogastridae). Nematology 15213-231.

SusoyV.RagsdaleE.J.KanzakiN.SommerR.J. (2015). Rapid diversification associated with a macroevolutionary pulse of developmental plasticity. eLife DOI:10.7554/eLife.05463.

VölkJ. (1950). Die Nematoden der Regenwürmer und aasbesuchenden Käfer. Zoologische Jahrbücher (Systematik) 791-70.

WeingärtnerI. (1955). Versuch einer Neuordnung der Gattung Diplogaster Schulze 1857 (Nematoda). Zoologische Jahrbuecher (Systematik) 83248-317.

Figures

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    Adults of Rhabditidoides humicolus n. sp. Female on left, male on right.

  • View in gallery

    Adults of Rhabditidoides humicolus n. sp. A: Lateral view of stomatal opening region of male showing labial sensilla, cephalic sensilla and amphid; B: Left lateral view of stomatal region; C: Ventral view of stomatal region; D: Body surface structure showing secretory pore-like opening, deirid, lateral field, annulation and striation; E: Left lateral view of pharynx region; F, G: Relative positions of secretory pore-like opening and deirid showing variation among individuals; H: Right lateral view of anterior female gonad; I: Right lateral view of female tail; J: Ventral view of female anal region; K: Left lateral view of male tail; L: Ventral view of male tail; M: Left lateral view of spicule and gubernaculum; N: Ventral view of spicule and gubernaculum.

  • View in gallery

    Right lateral view of stomatal region of Rhabditidoides humicolus n. sp. in different focal planes. A: Surface showing amphid (am); B: Cheilostomatal cuticular ring (ch); C: Inner lip flaps (fl), pro-meso stegostomatal ring (p/m) and telostegostomatal ring (tel); D: Metastegostomatal tooth (met). This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

  • View in gallery

    Body surface of Rhabditidoides humicolus n. sp. in different focal planes. A-D: Relative position of secretory pore-like opening (p), deirid (d), basal bulb and excretory pore (ep). A, B and C, D are of the same individual in different focal planes; E: Subventral view of female vulval opening (v) and vulval papilla (vp); F, G: Left lateral view of female vulval region showing relative position of vulval papilla (vp in F) and vulval opening (v in G). This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

  • View in gallery

    Male tail characters of Rhabditidoides humicolus n. sp. A-C: Left lateral view of male tail showing genital papillae and phasmid (ad = 5th lateral pair of papillae, pd = 8th dorsal pair of papillae, v1-v7 = subventral pairs of papillae, v3d = 3rd lateral pair of papillae papilla, vs = mid-ventral single papilla, ph = phasmid); D-F: Right lateral view of spicule and gubernaculum in different focal planes; G: Ventral view of spicule; H: Ventral view of gubernaculum. This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

  • View in gallery

    Right lateral view of female anal region of Rhabditidoides humicolus n. sp. in different focal planes. A: Body surface showing phasmid (ph); B: Right subventral rectal gland (sv); C: Dorsal rectal gland (d) and anal opening (a). This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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