Subanguina moxae, belonging to the subfamily Anguininae, is an obligate parasite of Artemisia plants, which are widely used as cooking herbs and in traditional medicine in East Asia. Because the nematode is distributed throughout East Russia and East Asia, there is concern about the potential for significant damage to commercial farming; however, details about its biology remain unclear. To investigate the genetic diversity of S. moxae, we developed 2243 simple sequence repeat (SSR) markers using Illumina short reads of the genomic DNA. We validated 100 randomly selected markers indicating their robustness and examined polymorphisms among nematode populations sampled from four different locations in Japan. These SSR markers will be a useful tool for understanding the population structure and transmission patterns of this parasitic nematode.
Universal primers for fluorescent labelling of PCR fragments – an efficient and cost effective approach to genotyping by fluorescence.
Molecular Ecology Resources12456-463.
Genome-wide survey and analysis of microsatellites in nematodes, with a focus on the plant-parasitic species Meloidogyne incognita.
DaigoK.MakiharaH.OguraN. (2007). Subanguina moxae isolated from the leaf gall of the common mugwort Artemisia princeps in Japan. Meiji Japan Bulletin of the School of Agriculture Meiji University Tokyo.
The nucleotide sequence analysis of ITS1-5.8SrDNA-ITS2 region of Subanguina moxae collected in Japan.
Origin and genetic diversity of Western European populations of the potato cyst nematode (Globodera pallida) inferred from mitochondrial sequences and microsatellite loci.
SubbotinS.A.KrallE.L.RileyI.T.ChizhovV.N.StaelensA.De LooseM.MoensM. (2004).
Evolution of the gall-forming plant parasitic nematodes (Tylenchida: Anguinidae) and their relationships with hosts as inferred from Internal Transcribed Spacer sequences of nuclear ribosomal DNA.
Molecular Phylogenetics and Evolution30226-235.
A second generation framework for the analysis of microsatellites in expressed sequence tags and the development of EST-SSR markers for a conifer, Cryptomeria japonica.
VallesJ.McArthurE.D. (2001). Artemisia systematics and phylogeny: cytogenetic and molecular insights. In: McArthur E.D. & Fairbanks D.J. (Eds). Shrubland ecosystem genetics and biodiversity. Provo UT USA Conference proceedings June 13-15 2000 pp. 67-74.
van MegenH.van den ElsenS.HoltermanM.KarssenG.MooymanP.BongersT.HolovachovO.BakkerJ.HelderJ. (2009).
A phylogenetic tree of nematodes based on about 1200 full-length small subunit ribosomal DNA sequences.
On a new species of shoot gall nematode (Tylenchidae: Anguina) found from the galls on the leaves of Moxa (Artemisia saiatica Nakai).
Bulletin of the Faculty of Agriculture of Saga University261-7.