An undescribed Bursaphelenchus species was isolated and cultured from Dorcus titanus sakishimanus collected during a field survey of the insect-associated nematodes in subtropical Japan. The stag beetles were collected from Ishigaki Island, Okinawa, Japan, and dissected to examine their nematode associates. Then the dissected bodies were individually transferred to 2.0% agar plates, and nematode propagation on the plates was periodically examined. Nematodes were first recovered from the agar plate, i.e., the number of nematodes carried by the beetle was low, and infection was not confirmed during dissection. The new species was morphologically and phylogenetically (on a molecular basis) close to B. gerberae, which was isolated from the palm weevil, Rhynchophorus palmarum (Curculionidae), from tropical America, and to other weevil-associated Bursaphelenchus species. However, the new species can be distinguished from its close relatives by its typological characters, e.g., long and slender female tail and male spicule morphology as well as phylogenetic status inferred from the near-full-length of the small subunit (SSU) of the ribosomal RNA gene. The new species is described and illustrated herein as B. sakishimanus n. sp. and its molecular profiles, near-full-length SSU, and D2-D3 expansion segments of the large subunit of ribosomal RNA are described. This is the second Bursaphelenchus species associated with stag beetles (Lucanidae).
Bursaphelenchusabietinus sp. n. (Nematoda: Parasitaphelenchidae) associated with fir bark beetles (Pityokteines spp.) from declining trees in Austria.
Russian Journal of Nematology81-6.
Bursaphelenchus doui sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Taiwan and South Korea – a new species of the xylophilus group.
Russian Journal of Nematology1319-27.
Morphology and systematics of Bursaphelenchus gerberae n. sp. (Nematoda: Parasitaphelenchidae), a rare associate of the palm weevil, Rhynchophorus palmarum, in Trinidad.
Handling, fixing, staining and mounting nematodes. In:
Laboratory methods for work with plant and soil nematodes. London, UKHer Majesty’s Stationery Office pp.
Taxonomy and systematics of the nematode genus Bursaphelenchus (Nematoda: Parasitaphelenchidae). In:
Pine wilt disease. Tokyo, JapanSpringer pp.
Description of Bursaphelenchus kiyoharai n. sp. (Tylenchina: Aphelenchoididae) with remarks on the taxonomic framework of the Parasitaphelenchinae Rühm, 1956 and Aphelenchoidinae Fuchs, 1937.
Bursaphelenchus tadamiensis n. sp. (Nematoda: Aphelenchoididae), isolated from a stag beetle, Dorcus striatipennis (Coleoptera: Lucanidae), from Japan.
New plant-parasitic nematode from the mostly mycophagous genus Bursaphelenchus discovered inside figs in Japan.
PLoS ONE9e99241. DOI:10.1371journal.pone.0099241.
Bursaphelenchusosumiana n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from dead Pinusarmandii var. amamiana in Osumi Islands in Japan.
A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchus xylophilus by loop mediated isothermal amplification (LAMP).
Nematode parasites and associates of Dendroctonus spp. and Trypodendronlineatum (Coleoptera: Scolytidae), with a description of Bursaphelenchusvaricauda n. sp.Journal of Nematology15312-318.
Description of Bursaphelenchus populi sp. n. (Nematoda: Parasitaphelenchidae), a new member of the xylophilus group from aspen, Populus tremula L., in Europe.
Bursaphelenchus trypophloei sp. n. (Nematoda: Parasitaphelenchinae) – an associate of the bark beetle, Trypophloeus asperatus (Gyll.) (Coleoptera: Curculionidae: Scolytinae), in aspen, Populus tremula L.Nematology13619-636.
Bursaphelenchus fagi sp. n. (Nematoda: Parasitaphelenchidae), an insect-pathogenic nematode in the Malpighian tubules of the bark beetle, Taphrorychus bicolor (Herbst.) (Coleoptera: Curculionidae: Scolytinae), in European beech, Fagus silvatica L.Nematology16591-606.
Bursaphelenchusmasseyi sp. n. (Nematoda: Parasitaphelenchinae) – a nematode associate of the bark beetle, Trypophloeuspopuli Hopkins (Coleoptera: Curculionidae: Scolytinae), in aspen, Populustremuloides Michx, affected by sudden aspen decline in Colorado.
Phylogenetic relationships among Bursaphelenchus species (Nematoda: Parasitaphelenchidae) inferred from nuclear ribosomal and mitochondrial DNA sequence data.
Molecular Phylogenetics and Evolution431185-1197.