Pine wilt disease (PWD) caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus, is a destructive forest disease. Recent studies showed that bacteria associated with PWN might play important roles in PWD. In this study, the effects of endobacteria isolated from PWN on the development and virulence of PWN were evaluated. The results revealed that endobacteria isolated from high virulence PWN could partially promote the development of the nematode, while endobacteria from PWN with low virulence could inhibit the development of nematode. Both bacteria-free and non-sterilised nematodes were able to infect microcuttings of Pinus densiflora. The endobacterial isolates, Stenotrophomonas maltophilia NSBx.14 and Achromobacter xylosoxidans ssp. xylosoxidans NSBx.22, enhanced the virulence of low virulence PWN but reduced the virulence of high virulence PWN. The results suggest the pathogenicity of PWN is not affected by loss of bacteria and endobacteria might play a role in the development and virulence of PWN.
Metagenomic analysis of the pine wood nematode microbiome reveals a symbiotic relationship critical for xenobiotics degradation.
Purification of flagellin of Pseudomonas fluorescens GcM5-1A carried by the pine wood nematode, Bursaphelenchus xylophilus, and its in vitro toxicity to a suspension of cells of Pinus thunbergii.
Russian Journal of Nematology16151-157.
An endosymbiotic bacterium in a plant-parasitic nematode: member of a new Wolbachia supergroup.
International Journal for Parasitology391045-1054.
Accumulation of benzoic acid in suspension cultured cells of Pinus thunbergii Parl. in response to phenylacetic acid administration.
Bioscience Biotechnology and Biochemistry601410-1412.
Relationship between the pathogenicity of the pine wood nematode, Bursaphelenchus xylophilus and phenylacetic acid production.
Bioscience Biotechnology and Biochemistry601413-1415.
Isolation of pine wilting bacteria accompanying pine wood nematode, Bursaphelenchus xylophilus, and their toxic metabolites.
Science Reports of the Faculty of Agriculture Kobe University Japan871-7.
Re-isolation of the pathogens from wilted red pine seedlings inoculated with the bacterium-carrying nematode, and the cause of difference in pathogenicity among pine wood nematode isolates.
Science Reports of the Faculty of Agriculture Kobe University Japan881-5.
Migration speed of pine wood nematodes and activities of other microbes during the development of pine wilt disease in Pinus thunbergii.
Journal of the Japanese Forestry Society74383-389.
Effect of phosphate on the growth and toxin-producing ability of a bacterium isolated from pinewood nematode Bursaphelenchus lignicolus.
Science Reports of the Faculty of Agriculture Kobe University Japan5735-40.
Specific and functional diversity of endophytic bacteria from pine wood nematode Bursaphelenchus xylophilus with different virulence.
International Journal of Biological Sciences934-44.
Effects of Pseudomonas fluorescens flagellin on physiological and biochemical characteristics in the suspension cells of Pinus thunbergii.
European Journal of Plant Pathology136729-736.
Observation by transmission electron microscope and identification of endophytic bacteria isolated from Bursaphelenchus xylophilus and B. mucronatus.
Acta Microbiologica Sinica511071-1077.
Flagellin promotes propagation of pine wood nematode and its carrying Pseudomonas fluorescens GcM5-1A in callus of Pinus thunbergii through inducing cell death.
African Journal of Microbiology Research61322-1328.