Description of Ruehmaphelenchus juliae n. sp. (Tylenchina: Aphelenchoididae) isolated from an ambrosia beetle, Xylosandrus crassiusculus (Motschulsky), from South Florida

in Nematology
Restricted Access
Get Access to Full Text
Rent on DeepDyve

Have an Access Token?



Enter your access token to activate and access content online.

Please login and go to your personal user account to enter your access token.



Help

Have Institutional Access?



Access content through your institution. Any other coaching guidance?



Connect

During a survey of nematode associates of ambrosia beetles from dead and dying red bay and avocado trees affected by the laurel wilt epidemic in southern Florida, a Ruehmaphelenchus species was isolated from the non-native ambrosia beetle, Xylosandrus crassiusculus. The new species is characterised by its possession of an oral disc at the stoma opening, three lines in the lateral field, male spicule with clear dorsal and ventral limbs connected by elongated triangular cuticle, thin membrane-like tissue and cuticular bridge-like structure, conical tail with pointed tip of males and conical tail with digitate mucro of females. The new species is very similar to four previously described species: R. asiaticus, R. digitulus, R. thailandae and R. sirisus, and can be distinguished only by some minor morphological differences in male tail characters, i.e., spicule morphology, position of genital papillae and tail tip shape, and morphometric values. However, the new species is phylogenetically unique, i.e., it is the basal taxon of the Ruehmaphelenchus clade and close to Bursaphelenchus spp. Ruehmaphelenchus juliae n. sp. is therefore proposed based on its morphological diagnostic characters and molecular sequences of near-full-length of SSU, internal transcribed spacer region, D1, D2 and D3 expansion segments of LSU ribosomal RNA and partial mitochondrial COI genes.

Nematology

International Journal of Fundamental and Applied Nematological Research

Sections

References

AtkinsonT.H.FoltzJ.L.WilkinsonR.C.MizellR.F. (2012). Xylosandrus crassiusculus (Motschulsky) (Insecta: Coleoptera: Curculionidae: Scolytinae). Featured creatures. Fact sheet. University of Florida, available online at: http://entnemdept.ufl.edu/creatures/trees/asian_ambrosia_beetle.htm.

BajajH.K. (2012). Ruehmaphelenchus sirisus sp. n. and Albiziaphelenchus arthrorostrus gen. n., sp. n. (Aphelenchoididae: Aphelenchida) from bark of Albizia lebbeck (L.) Benth infected with bark borers. Indian Journal of Nematology 42, 118-124.

BiedermannP.H.W. (2007). Social behaviour in sib mating fungus farmers: intra- and interspecific cooperation in ambrosia beetles. Masterarbeit der Philosophisch-naturwissenschaftlichen Fakultät der Universität Bern, Bern, Switzerland.

BraaschH.GuJ.BurgermeisterW.BrandstetterM.MetgeK. (2006). Description of Ruehmaphelenchus asiaticus sp. n. (Nematoda: Aphelenchoididae) and remarks on the genus Ruehmaphelenchus. Journal of Nematode Morphology and Systematics 9, 39-43.

CarrilloD.DuncanR.E.PeñaJ.E. (2012). Ambrosia beetles (Curculionidae: Scolytinae) that breed in avocado wood in Florida. Florida Entomologist 95, 573-579.

CarrilloD.DuncanR.E.PloetzJ.N.CampbellA.F.PloetzR.C.PeñaJ.E. (2014). Lateral transfer of a phytopathogenic symbiont among native and exotic ambrosia beetles. Plant Pathology 63, 54-62.

DavisM.A.DuteR.R. (1997). Fungal associates of the Asian ambrosia beetle, Xylosandrus crassiusculus. Southern Nursery Association Research Conference 42, 106-112.

DuteR.R.MillerM.E.DavisM.A.WoodsF.M.McLeanK.S. (2002). Effects of ambrosia beetle attack on Cercis canadensis. IAWA Journal 23, 143-160.

FrankS.D.SadofC.S. (2011). Reducing insecticide volume and nontarget effects of ambrosia beetle management in nurseries. Journal of Economic Entomology 104, 1960-1968.

FuchsA.G. (1930). Neue an Borken- und Rüsselkäfer gebundene Nematoden, halbparasitische und Wohnungseinmieter. Zoologische Jahrbücher, Abteilung für Systematik, Ökologie und Geographie der Tiere 59, 505-646.

FuchsA.G. (1937). Neue arasitische und halbparasitische Nematoden bei Borkenkäfern und einige andere Nematoden. 1 Teil. Zoologische Jahrbücher, Abteilung für Systematik, Öekologie und Geographie der Tiere 70, 291-380.

Giblin-DavisR.M.Mundo-OcampoM.BaldwinJ.G.NordenB.B.BatraS.W.T. (1993). Description of Bursaphelenchus abruptus n. sp. (Nemata: Aphelenchoididae), an associate of a digger bee. Journal of Nematology 25, 161-172.

Giblin-DavisR.M.KanzakiN.DaviesK.A. (2013). Nematodes that ride insects: unforeseen consequences of arriving species. Florida Entomologist 96, 770-780.

GoodeyJ.B. (1963). Soil and freshwater nematodes, 2nd edition. London, UK, Methuen.

GuJ.WangJ. (2010). Description of Ruehmaphelenchus thailandae n. sp. (Nematoda: Aphelenchoididae) found in dunnage from Thailand. Nematology 12, 869-876.

GuJ.WangJ. (2012). Description of Ruehmaphelenchus digitulus sp. n. (Nematoda: Aphelenchoididae) found in packaging wood from Taiwan. Nematology 14, 489-498.

HooperD.J. (1986). Handling, fixing, staining and mounting nematodes. In: SoutheyJ.F. (Ed.). Laboratory methods for work with plant and soil nematodes. London, UK, Her Majesty’s Stationery Office, pp.  59-80.

HuelsenbeckJ.P.RonquistF. (2001). MR BAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17, 1754-1755.

HuntD.J. (1993). Aphelenchida, Longidoridae and Trichodoridae: their systematics and bionomics. Wallingford, UK, CAB International.

HuntD.J. (2008). A checklist of the Aphelenchoidea (Nematoda: Tylenchina). Journal of Nematode Morphology and Systematics 10(2007), 99-135.

KanzakiN.FutaiK. (2002). A PCR primer set for determination of phylogenetic relationships of Bursaphelenchus species within xylophilus group. Nematology 4, 35-41.

KanzakiN.MaeharaN.AikawaT.MasuyaH.Giblin-DavisR.M. (2011). Description of Bursaphelenchus kiyoharai n. sp. (Tylenchina: Aphelenchoididae) with remarks on the taxonomic framework of the Parasitaphelenchinae Rühm, 1956 and Aphelenchoidinae Fuchs, 1937. Nematology 13, 787-804.

KanzakiN.TakiH.MasuyaH.OkabeK.ChenC.-Y. (2013). Description of Ruehmaphelenchus formosanus n. sp. (Tylenchina: Aphelenchoididae) isolated from Euwallacea fornicates from Taiwan. Nematology 15, 895-906.

KanzakiN.LiH.-F.LanY.-C.Giblin-DavisR.M. (2014). Description of two Pseudaphelenchus species (Tylenchomorpha: Aphelenchoididae) associated with Asian termites and proposal of new subfamily Tylaphelenchinae n. subfam. Nematology 16, 963-978.

KanzakiN.OkabeK.KoboriY. (2015). Bursaphelenchus sakishimanus n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from a stag beetle, Dorcus titanus sakishimanus Nomura (Lucanidae), on Ishigaki Island in Japan. Nematology, in press (NEM 2887).

KatohK.MisawaK.KumaK.MiyataT. (2002). MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30, 3059-3066.

KikuchiT.AikawaT.OedaY.KarimN.KanzakiN. (2009). A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchus xylophilus by loop-mediated isothermal amplification (LAMP). Phytopathology 99, 1365-1369.

KirkendallL.R. (1983). The evolution of mating systems in bark and ambrosia beetles (Coleoptera: Scolytidae and Platypodidae). Zoological Journal of the Linnean Society 77, 293-352.

KörnerH. (1954). Die Nematodenfauna des vergehenden Holzes und ihre Beziehungen zu den Insekten. Zoologische Jahrbücher (Systematik) 82, 245-353.

LargetB.SimonD.L. (1999). Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16, 750-759.

NickleW.R. (1970). A taxonomic review of the genera of the Aphelenchoidea (Fuchs, 1937) Thorne, 1949 (Nematoda: Tylenchida). Journal of Nematology 2, 375-392.

PosadaD.CrandallK.A. (1998). MODELTEST: testing the model of DNA substitution. Bioinformatics 14, 817-818.

RühmW. (1955). Über einige an holzbrütende Ipiden gebundene Nematodenarten. Zoologischer Anzeiger 155, 70-83.

RühmW. (1956). Die Nematoden der Ipiden. Parasitologische SchriftenReihe 6, 1-435.

SkarbilovichT.S. (1947). [ Revision of the systematics of the nematode family Anguillulinidae Baylis and Daubney, 1926.] Doklady Akademy Nauk SSR 57, 307-308.

TanakaR.KikuchiT.AikawaT.KanzakiN. (2012). Simple and quick methods for nematode DNA preparation. Applied Entomology and Zoology 47, 291-294.

YeW.Giblin-DavisR.M.BraaschH.MorrisK.ThomasW.K. (2007). Phylogenetic relationships among Bursaphelenchus species (Nematoda: Parasitaphelenchidae) inferred from nuclear ribosomal and mitochondrial DNA sequence data. Molecular Phylogenetics and Evolution 43, 1185-1197.

Figures

  • Ruehmaphelenchus juliae n. sp. A: Female; B: Male.

    View in gallery
  • Ruehmaphelenchus juliae n. sp. A: Anterior region; B: Lip region and stylet; C: En face view showing oral disc; D: Body surface structure; E: Right lateral view of female gonad; F: Right lateral view of vulval region; G: Ventral view of female anus and rectum; H: Right lateral view of female tail; I: Left lateral view of male tail; J: Ventral view of male tail; K: Left lateral view of spicule; L: Ventral view of spicule; M: Early-stage egg; N: Late-stage egg.

    View in gallery
  • Ruehmaphelenchus juliae n. sp. A: Anterior region (ep = excretory pore; h = hemizonid); B-D: Lip and stylet in different focal planes (od = oral disc). This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

    View in gallery
  • Ruehmaphelenchus juliae n. sp. A-C: Right lateral view of male tail in different focal planes; D-F: Left lateral view of spicule (triangular piece is indicated with an arrowhead in E; and bridge connecting dorsal and ventral limb and notch-like separation at distal end of spicule are indicated with arrowheads in F); G-K: Ventral view of male tail in different focal planes. Genital papillae and cloacal opening are labelled P1, P2, P3, P4 and CO. This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

    View in gallery
  • Ruehmaphelenchus juliae n. sp. A-C: Right lateral view of female vulval region in different focal planes; D-F: Ventral view of female vulval region in different focal planes; G, H: Left lateral view of female tail in different focal planes; I-L: Early-stage egg in different focal planes; M, N: Late-stage egg in different focal planes. This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

    View in gallery
  • Molecular phylogenetic relationships among Ruehmaphelenchus, Sheraphelenchus and Bursaphelenchus species. Bayesian tree inferred from near-full-length of SSU and D2-D3 LSU under GTR + I + G model. lnL = 11 878.3496; freqA = 0.2674; freqC = 0.1694; freqG = 0.2535; freqT = 0.3097; R(a) = 1.4002; R(b) = 2.8221; R(c) = 1.3437; R(d) = 0.7125; R(e) = 5.5059; R(f) = 1; Pinvar = 0.4416; Shape = 0.4562 for near-full-SSU; lnL = 17 794.2188; freqA = 0.1815; freqC = 0.17; freqG = 0.3234; freqT = 0.3251; R(a) = 0.558; R(b) = 2.8917; R(c) = 1.0505; R(d) = 0.6494; R(e) = 4.8359; R(f) = 1; Pinvar = 0.2929; Shape = 0.8046 for D2-D3 LSU. Posterior probability values exceeding 50% are given on appropriate clades.

    View in gallery

Information

Content Metrics

Content Metrics

All Time Past Year Past 30 Days
Abstract Views 24 24 12
Full Text Views 5 5 5
PDF Downloads 0 0 0
EPUB Downloads 0 0 0