Cloning of a serine protease gene from the nematophagous fungus Esteya vermicola and expressed activity of the recombinant enzyme against Bursaphelenchus xylophilus
In: NematologySearch for other papers by Xuan Wang in
Current site
Google Scholar
Search for other papers by Tinglong Guan in
Current site
Google Scholar
Search for other papers by Long Zhang in
Current site
Google Scholar
Search for other papers by Hongmei Li in
Current site
Google Scholar
A serine protease gene Evsp was cloned from the nematophagous fungus Esteya vermicola with strong virulence against Bursaphelenchus xylophilus. The full-length cDNA of Evsp contains 2280 nucleotides with a 1656 bp ORF encoding a protein with 551 amino acids. The genomic Evsp includes two exons (396 bp and 1260 bp) separated by an intron (207 bp). There is only one copy of Evsp gene in the fungal genome. The deduced amino acids sequences of Evsp showed highly homology with the catalytic domains in subtilisin serine proteases. Phylogenetic analyses based on the protein sequences revealed that E. vermicola is separated from nematode-trapping fungi but close to other nematophagous and entomopathogenic fungi. The recombinant serine protease rEvsp was induced in Escherichia coli with expression vector pET28a(+). The tests of protease and nematicidal activities for the purified and refolded rEvsp indicated it is possibly involved in the fungal infection process against B. xylophilus.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Åhman J., Johansson T., Olsson M., Punt P.J., van den Hondel C.A., Tunlid A. (2002). Improving the pathogenicity of a nematode-trapping fungus by genetic engineering of a subtilisin with nematotoxic activity. Applied and Environmental Microbiology 68, 3408-3415.
-
Bagga S., Hu G., Screen S.E., Leger R.J. St (2004). Reconstructing the diversification of subtilisins in the pathogenic fungus Metarhizium anisopliae. Gene 324, 159-169.
-
Chen L.L., Liu L.J., Shi M., Song X.Y., Zheng C.Y., Chen X.L., Zhang Y.Z. (2009). Characterization and gene cloning of a novel serine protease with nematicidal activity from Trichoderma pseudokoningii SMF2. FEMS Microbiology Letters 299, 135-142.
-
Futai K. (2013). Pine wood nematode, Bursaphelenchus xylophilus. Annual Review of Phytopathology 51, 61-83.
-
Hainfeld J.F., Liu W., Halsey C.M., Freimuth P., Powell R.D. (1999). Ni-NTA-gold clusters target His-tagged proteins. Journal of Structural Biology 127, 185-198.
-
Kubátová A., Novotný D., Prášil K., Mráček Z. (2000). The nematophagous hyphomycete Esteya vermicola found in the Czech Republic. Czech Mycology 52, 227-235.
-
Laemmli U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
-
Li A.N., Li D.C. (2009). Cloning, expression and characterization of the serine protease gene from Chaetomium thermophilum. Journal of Applied Microbiology 106, 369-380.
-
Li H., Trinh P.Q., Waeyenberge L., Moens M. (2009). Characterization of Bursaphelenchus spp. isolated from packaging wood imported at Nanjing, China. Nematology 11, 375-408.
-
Li J., Yu L., Yang J.K., Dong L.Q., Tian B.Y., Yu Z.F., Liang L.M., Zhang Y., Wang X., Zhang K.Q. (2010). New insights into the evolution of subtilisin-like serine protease genes in Pezizomycotina. BMC Evolutionary Biology 10, 68.
-
Liou J.Y., Shih J.Y., Tzean S.S. (1999). Esteya, a new nematophagous genus from Taiwan, attacking the pinewood nematode (Bursaphelenchus xylophilus). Mycological Research 103, 242-248.
-
Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265-275.
-
Mamiya Y. (1983). Pathology of the pine wilt disease caused by Bursaphelenchus xylophilus. Annual Review of Phytopathology 21, 201-220.
-
Morton C.O., Hirsch P.R., Kerry B.R. (2004). Infection of plant-parasitic nematodes by nematophagous fungi – a review of the application of molecular biology to understand infection processes and to improve biological control. Nematology 6, 161-170.
-
Mota M.M., Vieira P. (2008). Pine wilt disease: a worldwide threat to forest ecosystems. Berlin, Germany, Springer.
-
Mota M.M., Braasch H., Bravo M.A., Penas A.C., Burgermeister W., Metge K., Sousa E. (1999). First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1, 727-734.
-
Robertson L., Arcos S.C., Escuer M., Merino R.S., Esparrago G., Abelleira A., Navas A. (2011). Incidence of the pinewood nematode Bursaphelenchus xylophilus Steiner & Buhrer, 1934 (Nickle, 1970) in Spain. Nematology 13, 755-757.
-
Sheng J., An K., Deng C., Li W., Bao X., Qiu D. (2006). Cloning a cuticle-degrading serine protease gene with biologic control function from Beauveria brongniartii and its expression in Escherichia coli. Current Microbiology 53, 124-128.
-
Shimazu M. (2004). Effects of temperature on growth of Beauveria bassiana F-263, a strain highly virulent to the Japanese pine sawyer, Monochamus alternatus, especially tolerance to high temperatures. Applied Entomology and Zoology 39, 469-475.
-
Singh S.M., Panda A.K. (2005). Solubilization and refolding of bacterial inclusion body proteins. Journal of Bioscience and Bioengineering 99, 303-310.
-
Suzuki K. (2002). Pine wilt disease – a threat to pine forest in Europe. Dendrobiology 48, 71-74.
-
Swofford D. (2002). PAUP∗ version 4.0. Phylogenetic analysis using parsimony (and other methods). Sunderland, MA, USA, Sinauer Associates.
-
Tunlid A., Jansson S. (1991). Proteases and their involvement in the infection and immobilization of nematodes by the nematophagous fungus Arthrobotrys oligospora. Applied and Environmental Microbiology 57, 2868-2872.
-
Wang B., Wu W., Liu X. (2007). Purification and characterization of a neutral serine protease with nematicidal activity from Hirsutella rhossiliensis. Mycopathologia 163, 169-176.
-
Wang C.Y., Fang Z.M., Sun B.S., Gu L.J., Zhang K.Q., Sung C.K. (2008). High infectivity of an endoparasitic fungus strain, Esteya vermicola, against nematodes. Journal of Microbiology 46, 380-389.
-
Wang C.Y., Fang Z.M., Wang Z., Zhang D.L., Gu L.J., Lee M.R., Sung C.K. (2011). Biological control of the pinewood nematode Bursaphelenchus xylophilus by application of the endoparasitic fungus Esteya vermicola. BioControl 56, 91-100.
-
Wang J., Wang J., Liu F., Pan C. (2010). Enhancing the virulence of Paecilomyces lilacinus against Meloidogyne incognita eggs by overexpression of a serine protease. Biotechnology Letters 32, 1159-1166.
-
Wang X., Wang T., Wang J., Guan T., Li H. (2014). Morphological, molecular and biological characterization of Esteya vermicola, a nematophagous fungus isolated from intercepted wood packing materials exported from Brazil. Mycoscience 55, 367-377.
-
Yang J.K., Huang X.W., Tian B.Y., Wang M., Niu Q.H., Zhang K.Q. (2005). Isolation and characterization of a serine protease from the nematophagous fungus, Lecanicillium psalliotae, displaying nematicidal activity. Biotechnology Letters 27, 1123-1128.
-
Yang J.K., Tian B.Y., Liang L.M., Zhang K.Q. (2007). Extracellular enzymes and the pathogenesis of nematophagous fungi. Applied Microbiology and Biotechnology 75, 21-31.
-
Yang J.K., Zhao X.N., Liang L.M., Xia Z.Y., Lei L.P., Niu X.M., Zou C.G., Zhang K.Q. (2011a). Overexpression of a cuticle-degrading protease Ver112 increases the nematicidal activity of Paecilomyces lilacinus. Applied Microbiology and Biotechnology 89, 1895-1903.
-
Yang J.K., Wang L., Ji X.L., Feng Y., Li X.M., Zou C.G., Xu J.P., Ren Y., Mi Q.L., Wu J.L. et al. (2011b). Genomic and proteomic analyses of the fungus Arthrobotrys oligospora provide insights into nematode-trap formation. PLoS Pathogen 7, e1002179.
-
Yang J.K., Liang L.M., Li J., Zhang K.Q. (2013). Nematicidal enzymes from microorganisms and their applications. Applied Microbiology and Biotechnology 97, 7081-7095.
-
Zhang Y., Li G.H., Zhang K.Q. (2011). A review on the research of nematophagous fungal species. Mycosystema 30, 836-845.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 470 | 55 | 3 |
| Full Text Views | 177 | 0 | 0 |
| PDF Views & Downloads | 30 | 1 | 0 |
Cloning of a serine protease gene from the nematophagous fungus Esteya vermicola and expressed activity of the recombinant enzyme against Bursaphelenchus xylophilus
In: NematologySearch for other papers by Xuan Wang in
Current site
Google Scholar
PubMed
Search for other papers by Tinglong Guan in
Current site
Google Scholar
PubMed
Search for other papers by Long Zhang in
Current site
Google Scholar
PubMed
Search for other papers by Hongmei Li in
Current site
Google Scholar
PubMed
A serine protease gene Evsp was cloned from the nematophagous fungus Esteya vermicola with strong virulence against Bursaphelenchus xylophilus. The full-length cDNA of Evsp contains 2280 nucleotides with a 1656 bp ORF encoding a protein with 551 amino acids. The genomic Evsp includes two exons (396 bp and 1260 bp) separated by an intron (207 bp). There is only one copy of Evsp gene in the fungal genome. The deduced amino acids sequences of Evsp showed highly homology with the catalytic domains in subtilisin serine proteases. Phylogenetic analyses based on the protein sequences revealed that E. vermicola is separated from nematode-trapping fungi but close to other nematophagous and entomopathogenic fungi. The recombinant serine protease rEvsp was induced in Escherichia coli with expression vector pET28a(+). The tests of protease and nematicidal activities for the purified and refolded rEvsp indicated it is possibly involved in the fungal infection process against B. xylophilus.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 470 | 55 | 3 |
| Full Text Views | 177 | 0 | 0 |
| PDF Views & Downloads | 30 | 1 | 0 |
