Nothotylenchus persicus n. sp. (Nematoda: Anguinidae) from Kermanshah province, Iran

in Nematology
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A new species of the genus Nothotylenchus, N. persicus n. sp. was collected around the roots of grapevine and is described and illustrated herein based on morphological and molecular studies. The new species is characterised by a body length of 776-900 μm, delicate stylet 5-6 μm long, six lines in the lateral field, post-vulval uterine sac short, 10-18 μm long, female tail elongate-conoid with pointed terminus, and bursa covering 40-45% of tail length. Morphologically, N. persicus n. sp. appears closer to four known species of the genus, namely: N. hexaglyphus, N. affinis, N. medians and N. taylori. The results of phylogenetic analyses based on sequences of D2-D3 expansion region of 28S rRNA gene confirmed the close molecular relationship between N. persicus n. sp. and other anguinids, but Nothotylenchus claded separately from Ditylenchus species.

Nothotylenchus persicus n. sp. (Nematoda: Anguinidae) from Kermanshah province, Iran

in Nematology



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    Light micrographs of Nothotylenchus persicus n. sp. A: Female anterior body; B: Lateral field; C: Female stylet; D, E: Female pharyngeal region showing excretory pore (arrowhead); F: Vulval region and post-vulval uterine sac (arrowhead); G: Pharyngeal basal bulb and excretory pore (arrowhead); H: Posterior body of male; I: Posterior body of female. (Scale bars = 20 μm.)

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    Line drawing of Nothotylenchus persicus n. sp. A: Female entire body; B: Male entire body; C, G: Female pharyngeal region; D: Female posterior body region; E: Female lip region and stylet; F: Vulval region; H: Lateral field at mid-body; I: Male posterior region; J: Female posterior region. (Scale bars = 30 μm.)

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    The 50% majority rule consensus trees from Bayesian analysis generated from the D2-D3 of 28S rRNA gene dataset of anguinid nematodes with the TVM + I + G model. Posterior probabilities ⩾70% are given for appropriate clades (− = posterior probability < 70%). Newly obtained sequence is in bold letters. (Scale bar = expected changes per site.)


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