Early detection and quantification of Meloidogyne hapla in soil is essential for effective disease management. The purpose of this study was to develop a real-time PCR assay for detection of M. hapla in soil. Primers and a TaqMan probe were designed for M. hapla detection. The assay detected M. hapla and showed no significant amplification of DNA from non-target nematodes. The assay was able to detect M. hapla in a background of plant and soil DNA. A dilution series of M. hapla eggs in soil showed a high correlation (, ) with Ct values. The assay could predict root-knot development in carrots by testing soils before planting. The assay could be useful for management decisions in carrot cultivation.
Molecular diagnostic key for identification of single juveniles of seven common and economically important species of root-knot nematode (Meloidogyne spp.).
Detection and quantification of root-knot nematode (Meloidogyne javanica), lesion nematode (Pratylenchus zeae) and dagger nematode (Xiphinema elongatum) parasites of sugarcane using real-time PCR.
Molecular and Cellular Probes22168-176.
Root-knot nematode (Meloidogyne) management in vegetable crop production: the challenge of an agronomic system analysis.
Investigation and integrated molecular diagnosis of root-knot nematodes in Panax notoginseng root in the field.
European Journal of Plant Pathology137667-675.
Comparison of calibration curves prepared by soil compaction and ball milling methods for direct quantification of the potato cyst nematode Globodera rostochiensis in soil.
Morphological and molecular evaluation of a Meloidogyne hapla population damaging coffee (Coffea arabica) in Maui, Hawaii.
Journal of Nematology37136-145.
Development of a direct quantitative detection method for Meloidogyne incognita in sandy soils and its application to sweet potato cultivated fields in Tokushima prefecture, Japan.
Evaluating high-throughput sequencing as a method for metagenomic analysis of nematode diversity.
Molecular Ecology Resources91439-1450.
Van GhelderC.ReidA.KenyonD.EsmenjaudD. (2014).
Development of a real-time PCR method for the detection of the dagger nematodes Xiphinema index, X. diversicaudatum, X. vuittenezi and X. italiae, and for the quantification of X. index numbers.
A fast PCR assay to identify Meloidogyne hapla, M. chitwoodi, and M. fallax, and to sensitively differentiate them from each other and from M. incognita in mixtures.
Fundamental and Applied Nematology20505-511.
Identification of Meloidogyne chitwoodi, M. fallax and M. hapla based on SCAR-PCR: a powerful way of enabling reliable identification of populations or individuals that share common traits.
European Journal of Plant Pathology106283-290.