Steinernema fabii n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from South Africa

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A new species of entomopathogenic nematode, Steinernema fabii n. sp., was isolated by trapping with wax moth (Galleria mellonella) larvae from soil in an Acacia mearnsii plantation in the Mpumalanga province of South Africa. The new species is morphologically characterised by the length of the infective juvenile (IJ) of 641 (590-697) μm, by a tail length of 58 (52-64) μm, ratio a = 24 (21-41), H% = 53 (37-61) and E% = 93 (83-105). The pattern of the lateral field of the IJ of the new species is 2, 5, 2 ridges (3, 6, 3 incisures). The male of the first generation can be recognised by the long spicule of 90 (79-106) μm and gubernaculum of 66 (56-77) μm; D% = 64 (52-75) and GS% = 73 (63-86). The first generation female can be recognised by a protruding vulva with a short, double-flapped epiptygmata, and the lack of a postanal swelling, while the second generation differs in having a postanal swelling and a conical, sharply pointed tail. Analysis of DNA sequences for the ITS and D2-D3 gene regions showed S. fabii n. sp. to differ from all other Steinernema species and to belong to a new monophyletic group, the ‘Cameroonian’ clade, consisting of S. cameroonense, S. nyetense, S. sacchari and S. fabii n. sp., all from the African continent. This group is closely related to species in the feltiae-kraussei-oregonense Clade III.

Steinernema fabii n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from South Africa

in Nematology

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References

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Figures

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    Steinernema fabii n. sp. A-C, first generation female. A: Anterior region; B: Tail region; C: Vulva. First generation male (D-F). D: Ventral view of tail; E: Lateral view of tail region; F: Spicule. G: Tail of second generation female. Infective juvenile. H: Anterior region; I: Tail region. (Scale bars: A-D = 50 μm; E, F, I = 20 μm; G, H = 10 μm.)

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    Male of Steinernema fabii n. sp., first generation male. A: En face view (a = amphidial aperture); B: Lateral view of tail; C: Spicule and gubernaculum of tail of second generation; D: Lateral view of tail region with genital papillae; E: Spicule; F: Gubernaculum. The papillae in B and D are numbered; S = single mid-ventral papilla. (Scale bars: A = 5 μm; B, C, E, F = 20 μm; D = 50 μm.)

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    Female Steinernema fabii n. sp. First generation. A: Anterior region showing pharynx and excretory pore (ep); B, D: Vulva; C: En face view; E, G: Tail. F, H: Second generation female tail with postanal swelling. (Scale bars: A, E, F = 20 μm; B, C, G, H = 5 μm; D = 10 μm.)

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    Infective juvenile of Steinernema fabii n. sp. A: Anterior end showing excretory pore (EP) and hemizonid (h); B: Tail region showing hyaline region; C: Anterior region showing four cephalic papillae and two amphidial apertures (a); D: Tail with anus (an) and phasmid (p); E: Splitting of ridges in lateral field from two to five (from anterior; ridges numbered); F: Five ridges in lateral field at mid-body (ridges numbered). (Scale bars: A, B, E, F = 5 μm; C = 2 μm; D: 10 μm.)

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    Phylogenetic relationships of Steinernema fabii n. sp. with 37 species of Steinernema based on the ITS-rDNA sequences from GenBank. Caenorhabditis elegans (EU131007) was used as out group. Numbers at the nodes represent bootstrap proportion for Maximum Parsimony of 50% or more.

  • View in gallery

    Phylogenetic relationships of Steinernema fabii n. sp. with 41 species of Steinernema based on the D2-D3 rDNA sequences from GenBank. Cervidellus alutus (AF331911) was used as out group. Numbers at the nodes represent bootstrap proportion for Maximum Parsimony of 50% or more.

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