Genetic diversity of Bursaphelenchus cocophilus in South America
In: Nematology4NemaLab/ICAAM – Instituto de Ciências Agrárias e Ambientais Mediterrânicas & Departamento de Biologia, Universidade de Évora, Núcleo da Mitra, Ap. 94, 7002-554 Évora, Portugal
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5Department of Environmental Biology, Chubu University, Kasugai, Japan
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6Departamento de Ciências da Vida, Universidade Lusófona de Humanidades e Tecnologias, EPCV, C. Grande 376, 1749-024 Lisbon, Portugal
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Molecular characterisation of Bursaphelenchus cocophilus, the causal agent of ‘red ring disease’, is imperative for efficient identification procedures in Brazil and Colombia, because quarantine species such as B. xylophilus and B. mucronatus are already listed in both countries. ITS-1/2 region and D2-D3 segment of LSU rDNA were used to characterise isolates of B. cocophilus obtained from coconut plantations in Brazil and Colombia. Results from ITS-1/2 and LSU rDNA regions showed that all isolates of B. cocophilus from Brazil and Colombia formed a monophyletic group. The LSU rDNA region indicated that all isolates formed a single monophyletic group with high Bayesian posterior probability (100%). This is the first study on ITS-1/2 for the characterisation of B. cocophilus populations. A species-specific primer was designed for identification of B. cocophilus.
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Genetic diversity of Bursaphelenchus cocophilus in South America
In: Nematology4NemaLab/ICAAM – Instituto de Ciências Agrárias e Ambientais Mediterrânicas & Departamento de Biologia, Universidade de Évora, Núcleo da Mitra, Ap. 94, 7002-554 Évora, Portugal
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5Department of Environmental Biology, Chubu University, Kasugai, Japan
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6Departamento de Ciências da Vida, Universidade Lusófona de Humanidades e Tecnologias, EPCV, C. Grande 376, 1749-024 Lisbon, Portugal
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Molecular characterisation of Bursaphelenchus cocophilus, the causal agent of ‘red ring disease’, is imperative for efficient identification procedures in Brazil and Colombia, because quarantine species such as B. xylophilus and B. mucronatus are already listed in both countries. ITS-1/2 region and D2-D3 segment of LSU rDNA were used to characterise isolates of B. cocophilus obtained from coconut plantations in Brazil and Colombia. Results from ITS-1/2 and LSU rDNA regions showed that all isolates of B. cocophilus from Brazil and Colombia formed a monophyletic group. The LSU rDNA region indicated that all isolates formed a single monophyletic group with high Bayesian posterior probability (100%). This is the first study on ITS-1/2 for the characterisation of B. cocophilus populations. A species-specific primer was designed for identification of B. cocophilus.
| All Time | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 686 | 183 | 16 |
| Full Text Views | 185 | 5 | 0 |
| PDF Views & Downloads | 32 | 13 | 0 |