Liquid culture production of microsclerotia of Purpureocillium lilacinum for use as bionematicide

in Nematology
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The production of microsclerotia by Purpureocillium lilacinum in liquid culture for use as a biocontrol agent for management of root-knot nematode, Meloidogyne incognita, has not been described. To investigate the potential for microsclerotia production, P. lilacinum strain CQPL01 was cultured in liquid media containing various concentrations of ferrous sulphate. Under these conditions mycelia began to form microsclerotia. The maximum yield (11.8 × 104 microsclerotia ml−1) was obtained in medium containing 0.2 g l−1 ferrous sulphate and the greatest production of conidia (1.3 × 108 conidia g−1) was obtained by the culture of air-dried microsclerotia in aqueous agar medium. Subsequently, the viability of microsclerotia, including stress resistance, storage stability and pathogenicity against M. incognita, was investigated. The microsclerotia exhibited excellent nematophagous ability and greater thermotolerance and UV-B radiation tolerance compared to conidia. These results suggested that microsclerotia propagules might be superior to the use of conidia in P. lilacinum biocontrol products.

Liquid culture production of microsclerotia of Purpureocillium lilacinum for use as bionematicide

in Nematology



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    Effect of ferrous sulphate on (A) MS yields and (B) biomass of Purpureocillium lilacinum. Bars are standard errors. Mean values followed by different letters are significantly different (P<0.05; Duncan’s multiple range test).

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    Liquid cultures of Purpureocillium lilacinum MS A: Formation of brown MS bodies in amended media culture (scale bar: 100 μM). B: The conidia yield of dried MS after incubation on agar for 12 days at 25°C. This figure is published in colour in the online edition of this journal, which can be accessed via

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    The conidia produced by air-dried MS of Purpureocillium lilacinum derived from cultures with various concentrations of Fe2+. Bars are standard errors. Mean values followed by different letters are significantly different (P<0.05; Duncan’s multiple range test).

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    Germination of (A) air-dried MS and (B) conidia of Purpureocillium lilacinum induced by UV radiation. Bars are standard errors. Mean values followed by different letters are significantly different (P<0.05) when compared to the results observed at time zero.

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    Germination rates of air-dried MS of Purpureocillium lilacinum stored at room temperature. The MS were counted after germination for (A) 24 h and (B) 48 h. Bars are standard errors. Mean values followed by different letters are significantly different (P<0.05) compared to results observed at time 0.

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