The production of microsclerotia by Purpureocillium lilacinum in liquid culture for use as a biocontrol agent for management of root-knot nematode, Meloidogyne incognita, has not been described. To investigate the potential for microsclerotia production, P. lilacinum strain CQPL01 was cultured in liquid media containing various concentrations of ferrous sulphate. Under these conditions mycelia began to form microsclerotia. The maximum yield (11.8 × 104 microsclerotia ml−1) was obtained in medium containing 0.2 g l−1 ferrous sulphate and the greatest production of conidia (1.3 × 108 conidia g−1) was obtained by the culture of air-dried microsclerotia in aqueous agar medium. Subsequently, the viability of microsclerotia, including stress resistance, storage stability and pathogenicity against M. incognita, was investigated. The microsclerotia exhibited excellent nematophagous ability and greater thermotolerance and UV-B radiation tolerance compared to conidia. These results suggested that microsclerotia propagules might be superior to the use of conidia in P. lilacinum biocontrol products.
The combined effect of the application of a biocontrol agent Paecilomyces lilacinus, with various practices for the control of root-knot nematodes.
Development of a new management strategy for the control of root-knot nematodes (Meloidogyne spp.) in organic vegetable production.
Pest Management Science59183-189.
Debilitation in conidia of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae and implication with respect to viability determinations and mycopesticide quality assessment.
Journal of Invertebrate Pathology10574-83.
Phenotypic and genetic characterization of Paecilomyces lilacinus strains with biocontrol activity against root-knot nematodes.
Canadian Journal of Microbiology46775-783.
Effect of oxidative stress and exogenous β-carotene on sclerotial differentiation and carotenoid yield of Penicillium sp. PT95.
Letters in Applied Microbiology40412-417.
Production of microsclerotia of the fungal entomopathogen Metarhizium anisopliae and their potential for use as a biocontrol agent for soil-inhabiting insects.
Effect of Meloidogyne incognita inoculum density and application rate of Paecilomyces lilacinus strain 251 on biocontrol efficacy and colonization of egg masses analyzed by real-time quantitative PCR.
Liquid culture production of microsclerotia and submerged conidia by Trichoderma harzianum active against damping-off disease caused by Rhizoctonia solani.
NADH:flavin oxidoreductase/NADH oxidase and ROS regulate microsclerotium development in Nomuraea rileyi.
World Journal of Microbiology and Biotechnology301927-1935.
Production of leucinostatins and nematicidal activity of Australian isolates of Paecilomyces lilacinus (Thom) Samson.
Letters in Applied Microbiology38271-276.
Optimization of culture medium for microsclerotia production by Nomuraea rileyi and analysis of their viability for use as a mycoinsecticide.
Role of two Nomuraea rileyi transmembrane sensors Sholp and Sln1p in adaptation to stress due to changing culture conditions during microsclerotia development.
World Journal of Microbiology and Biotechnology31477-485.
Relationship between thermotolerance and hydrophobin-like proteins in aerial conidia of Beauveria bassiana and Paecilomyces fumosoroseus as fungal biocontrol agents.
Journal of Invertebrate Pathology97323-331.