Tylenchid entomoparasites isolated from Spondylis buprestoides (L.) and Asemum striatum (L.) (Coleoptera: Cerambycidae)

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Tylenchid entomoparasites were isolated from two longhorn beetle species, Spondylis buprestoides from Tsukuba, Japan, and Asemum striatum from Ithaca, Tompkins County, NY, USA. Parasitic females and juveniles were obtained from S. buprestoides, where the female has a large body (5-6 mm long), clear stylet retracted into the body and degenerate anus and rectum, similar to some neotylenchids and allantonematids. By contrast, only stylet-bearing parasitic juveniles were found in eggs of A. striatum, and detailed morphological analysis was not done for this isolate. These two isolates of parasites were molecularly characterised, and analysed for their phylogenetic status based on the small subunit of the ribosomal RNA gene. The phylogenetic inference suggested that the two parasitic nematodes are close to each other, and to the siricidicola superspecies of the genus Deladenus, i.e., these two species and Deladenus form a well-supported clade. Because these two host beetle species belong to the same cerambycid subfamily (Spondylinae) from disparate locations, a subfamily-associated nematode parasite lineage may be revealed by further field surveys.

Tylenchid entomoparasites isolated from Spondylis buprestoides (L.) and Asemum striatum (L.) (Coleoptera: Cerambycidae)

in Nematology



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    Entomoparasitic female isolated from the body cavity of Spondylis buprestoides. A: Entire body; B: Anterior section showing excretory pore (ep), ovary (ov), and uterus (ut); C, D: Stomatal region imaged in different focal planes to show stylet tip (st), knob (sk), and dorsal pharyngeal gland orifice (pgo); E, F: Excretory system imaged in different focal planes to show excretory pore (ep) and secretory cell (sc); G: Early-stage embryo in anterior of uterus; H: Early-stage unhatched juvenile in middle of uterus; I: Hatched juveniles in posterior uterus; J: Annulations on surface; K-M: Posterior region of body in different focal planes to show vulval opening (vo), degenerate anus and rectum (a), and tail spike (sp). This figure is published in colour in the online edition of this journal, which can be accessed via http://booksandjournals.brillonline.com/content/journals/15685411.

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    Molecular phylogenetic relationship among several tylenchid insect-parasitic species. The Maximum Likelihood tree was inferred from near-full-length SSU. The substitution model was selected automatically as GTR + I + G (lnL = − 7774.74, AIC = 15747.49) and parameters are as follows: (freqA = 0.261; freqC = 0.198; freqG = 0.260; freqT = 0.280; R(a) = 1.1473; R(b) = 3.5819; R(c) = 1.8986; R(d) = 0.4754; R(e) = 6.3587; R(f) = 1; Pinvar = 0.457; Shape = 0.651). Bootstrap support exceeding 50% is shown for appropriate clades.

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