Tylenchid entomoparasites were isolated from two longhorn beetle species, Spondylis buprestoides from Tsukuba, Japan, and Asemum striatum from Ithaca, Tompkins County, NY, USA. Parasitic females and juveniles were obtained from S. buprestoides, where the female has a large body (5-6 mm long), clear stylet retracted into the body and degenerate anus and rectum, similar to some neotylenchids and allantonematids. By contrast, only stylet-bearing parasitic juveniles were found in eggs of A. striatum, and detailed morphological analysis was not done for this isolate. These two isolates of parasites were molecularly characterised, and analysed for their phylogenetic status based on the small subunit of the ribosomal RNA gene. The phylogenetic inference suggested that the two parasitic nematodes are close to each other, and to the siricidicola superspecies of the genus Deladenus, i.e., these two species and Deladenus form a well-supported clade. Because these two host beetle species belong to the same cerambycid subfamily (Spondylinae) from disparate locations, a subfamily-associated nematode parasite lineage may be revealed by further field surveys.
New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0.
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Laboratory methods for work with plant and soil nematodes. London, UKHer Majesty’s Stationery Office pp.
Description of Ektaphelenchoidesspondylis n. sp. (Nematoda: Ektaphelenchinae) isolated from Spondylisbuprestoides (Coleoptera: Cerambycidae) in Japan.
A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchus xylophilus by loop-mediated isothermal amplification (LAMP).
Phylogenetic analysis of Deladenus nematodes parasitizing northeastern North American Sirex species.
Journal of Invertebrate Pathology113177-183.
Phylogenetic relationships among Bursaphelenchus species (Nematoda: Parasitaphelenchidae) inferred from nuclear ribosomal and mitochondrial DNA sequence data.
Molecular Phylogenetics and Evolution431185-1197.