Diversity of root-lesion nematodes (Pratylenchus spp.) associated with wheat (Triticum aestivum and T. durum) in Morocco
In: Nematology2Institute for Agricultural and Fisheries Research, Plant, Crop Protection, Burg. Van Gansberghelaan 96, B-9820 Merelbeke, Belgium
3Laboratory for Agrozoology, Ghent University, Coupure links 653, B-9000 Ghent, Belgium
Search for other papers by Fouad Mokrini in
Current site
Google Scholar
Search for other papers by Lieven Waeyenberge in
Current site
Google Scholar
4Department of Biology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium
Search for other papers by Nicole Viaene in
Current site
Google Scholar
Search for other papers by Fouad Abbad Andaloussi in
Current site
Google Scholar
3Laboratory for Agrozoology, Ghent University, Coupure links 653, B-9000 Ghent, Belgium
Search for other papers by Maurice Moens in
Current site
Google Scholar
Root-lesion nematodes (Pratylenchus) have a worldwide distribution and cause severe production constraints on numerous important crops. During a survey of the wheat-growing area of Morocco, 17 populations of root-lesion nematode were collected. They were identified on the basis of their morphological and morphometric characters, and by molecular methods. Microscopic observations of females and males demonstrated the occurrence of P. penetrans in 13 of the 17 samples; P. thornei and P. pseudocoffeae were detected in four samples from Zaers and a single sample from Settat, respectively. A duplex PCR primer set was used to confirm the presence of P. penetrans while the species-specific forward primer PTHO and the common reverse primer D3B were used for P. thornei. For the remaining populations, the D2-D3 expansion segments of the 28S rRNA gene were amplified and the obtained sequences were compared with those of Pratylenchus species in the GenBank database. This comparison confirmed the morphological identifications and revealed a population of P. pinguicaudatus. The study of the phylogenetic relationship of the Moroccan Pratylenchus populations showed a high similarity (99-100%) between all P. penetrans populations. The population dynamics of six Pratylenchus populations from Morocco were evaluated on carrot disk cultures at 4, 8 and 12 weeks after inoculation, and at 10, 15, 20 and 25°C. The optimum temperature for reproduction of all populations was 20°C. After 8 weeks at this temperature, nematode numbers increased up to 458-fold, 310-fold and 252-fold for the four populations of P. penetrans, the P. thornei and the P. pseudocoffeae population, respectively.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Al-Banna L., Ploeg A.T., Williamson V.M., Kaloshian I. (2004). Discrimination of six Pratylenchus species using PCR and species-specific primers. Journal of Nematology 36, 142-146.
-
Ammati M. (1987). Nematode status on food legumes in Morocco. In: Saxena M.C., Sikora R.A., Srivastava J.P. (Eds). Nematodes parasitic to cereals and legumes in temperate semi-arid regions. Aleppo, Syria, International Centre for Agricultural Research in the Dry Areas (ICARDA), pp. 169-172.
-
(2013). Moroccan Ministry of Agriculture, report no. MO1303.7.
-
Ascota N., Malek R.B. (1979). Influence of temperature on population development of eight species of Pratylenchus on soybean. Journal of Nematology 11, 229-232.
-
Balaghi R., Jliben M., Tychon B., Eerens H. (2013). Agro-meteorological cereal yield forecasting in Morocco. Rabat, Morocco, INRA.
-
Carta L.K., Skantar A.M., Handoo Z.A. (2001). Molecular, morphological and thermal characters of 19 Pratylenchus spp. and relatives using the D3 segment of the nuclear LSU rRNA gene. Nematropica 31, 193-207.
-
Castillo P., Vovlas N. (2007). Pratylenchus (Nematoda: Pratylenchidae): diagnosis, biology, pathogenicity and management. Nematology Monographs and Perspectives 6 (Series Editors: Hunt D.J., Perry R.N.). Leiden, The Netherlands, Brill.
-
Castillo P., Jimenez Diaz R.M., Gomez-Barcina A., Vovlas N. (1995). Parasitism of the root-lesion nematode Pratylenchus thornei on chickpea. Plant Pathology 44, 728-733.
-
Castillo P., Gomez-Barcina A., Jimenez-Diaz R.M. (1996a). Plant parasitic nematodes associated with chickpea in southern Spain and effect of soil temperature on reproduction of Pratylenchus thornei. Nematologica 42, 211-219.
-
Castillo P., Gomez-Barcina A., Jimenez-Diaz R.M. (1996b). The effect of temperature on hatching and penetration of chickpea roots by Pratylenchus thornei. Plant Pathology 45, 310-315.
-
Castillo P., Vovlas N., Jimenez-Diaz R.M. (1998). Pathogenicity and histopathology of Pratylenchus thornei populations on selected chickpea genotypes. Plant Pathology 47, 370-376.
-
Dao D.F. (1970). Climatic influence of temperature on distribution pattern of plant parasitic and soil inhabiting nematodes. Mededelingen van de Landbouwhogeschool, Wageningen 70, 187.
-
de la Peña E., Moens M., Van Aelst A., Karssen G. (2006). Description of Pratylenchus dunensis sp. n. (Nematoda: Pratylenchidae) a root-lesion nematode associated with the dune grass Ammophila arenaria. Nematology 8, 79-88.
-
de la Peña E., Karssen G., Moens M. (2007). Distribution and diversity of root-lesion nematodes (Pratylenchus spp.) associated with Ammophila arenaria in coastal dunes of western Europe. Nematology 9, 881-901.
-
De Ley P., Felix M.A., Frisse L.M., Nadler S.A., Sternberg P.W., Thomas W.K. (1999). Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology 2, 591-612.
-
De Luca F., Fanelli E., Di Vito M., Reyes A., De Giorgi C. (2004). Comparison of the sequences of the D3 expansion of the 26S ribosomal genes reveals different degrees of heterogeneity in different populations and species of Pratylenchus from the Mediterranean region. European Journal of Plant Pathology 110, 949-957.
-
De Luca F., Reyes A., Troccoli A., Castillo P. (2011). Molecular variability and phylogenetic relationships among different species and populations of Pratylenchus (Nematoda: Pratylenchidae) as inferred from the analysis of the ITS-rDNA. European Journal of Plant Pathology 130, 415-426.
-
Doucet M.E., Lax P., Di Rienzo J.A., Pinochet J., Baujard P. (2001). Temperature-induced morphological variability in an isolate of Pratylenchus vulnus Allen & Jensen, 1951 (Nematoda: Tylenchida). Nematology 3, 1-8.
-
Duncan L.W., Inserra R.N., Thomas W.K., Dunn D., Mustika L., Frisse Mendes M.L., Morris K., Kaplan D.T. (1999). Molecular and morphological analysis of isolates of Pratylenchus coffeae and closely related species. Nematropica 29, 61-80.
-
Fallas G.A., Sarah J.L., Fargette M. (1995). Reproductive fitness and pathogenicity of eight Radopholus similis populations on banana plants (Musa AAA cv. Poyo). Nematropica 25, 135-141.
-
Fayazi F., Farokh-Nejd R., Ahmad A.R., Bahmani Z. (2012). Molecular and morphometric identification of P. thornei and P. neglectus in southwest of Iran. Plant Pathology and Microbiology 3, 123.
-
Hafez S.I., Al-Rehiayani S., Thornton M., Sundararaj P. (1999). Differentiation of two geographically isolated populations of Pratylenchus neglectus based on their parasitism of potato and interaction with Verticillium dahlia. Nematropica 29, 25-36.
-
Hall T.A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis. Nucleic Acids Symposium Series 41, 95-98.
-
Hallman J., Viaene N. (2013). PM 7/119(1) nematode extraction. EPPO Bulletin 43, 471-495.
-
Handoo Z.A., Golden A.M. (1989). A key and diagnostic compendium to the species of the genus Pratylenchus Filipjev, 1936 (lesion nematodes). Journal of Nematology 21, 202-218.
-
Handoo Z.A., Carta L.K., Skantar A.M. (2001). Morphological and molecular characterisation of Pratylenchus arlingtoni n. sp., P. convallariae and P. fallax (Nematoda: Pratylenchidae). Nematology 3, 607-618.
-
Hendrickx G. (1995). An automated apparatus for extracting free-living nematode stages from soil. Nematologica 41, 308.
-
Holterman M., van der Wurff A., van den Elsen S., van Megen H., Bongers T., Holovachov O. (2006). Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution 23, 1792-1800.
-
Hossain A., Teixeira Da Silva J.A. (2012). Phenology, growth and yield of three wheat (Triticum aestivum L.) varieties as affected by high temperature stress. Notulae Scientia Biologicae 4, 97-109.
-
Ibrahim S.K., Perry R.N., Burrows P.R., Hooper D.J. (1994). Differentiation of species and populations of Aphelenchoides and of Ditylenchus angustus using a fragment of ribosomal DNA. Journal of Nematology 26, 412-421.
-
Inserra R.N., Troccoli A., Gozel U., Bernard E.C., Dunn D., Duncan L.W. (2007). Pratylenchus hippeastri n. sp. (Nematoda: Pratylenchidae) from amaryllis in Florida with notes on P. scribneri and P. hexincisus. Nematology 9, 25-42.
-
Jones J.T., Haegeman A., Danchin E.G.J., Gaur H.S., Helder J., Jones M.G.K., Kikuchi T., Manzanilla-López R., Palomares-Rius J., Wesemael W.M.L. et al. (2013). Top 10 plant-parasitic nematodes in molecular plant pathology. Molecular Plant Pathology 14, 946-961.
-
Kodira C.U., Ferris H. (1992). Effect of temperature on Pratylenchus neglectus and on its pathogenicity to barley. Journal of Nematology 24, 504-511.
-
Lasserre F., Rivoal R., Cook R. (1994). Interactions between Heterodera avenae and Pratylenchus neglectus on wheat. Journal of Nematology 26, 336-344.
-
Loof P.A.A. (1960). Taxonomic studies on the genus Pratylenchus (Nematoda: Pratylenchidae). Tijdschrift voor Plantenziekten 66, 29-90.
-
Loof P.A.A. (1991). The family Pratylenchidae Thorne, 1949. In: Nickle W.R. (Ed.). Manual of agricultural nematology. New York, NY, USA, Marcel Dekker, pp. 126-134.
-
Meskine M., Abbad Andaloussi F. (1992). Importance et répartition des nématodes phytoparasites associés aux cultures de blé et orge au Maroc. Al Awamia 80, 123-134.
-
Mizukubo T. (1992). Pratylenchus pseudocoffeae n. sp. (Nematoda: Pratylenchidae) from compositae plants in Japan. Applied Entomology and Zoology 27, 437-444.
-
Mizukubo T., Sano Z. (1997). Pratylenchus coffeae virulent races on sweet potato. Research Front 4, 2.
-
Mizukubo T., Oriu Y., Hanada K., Sano Z. (2003). Micro evolutionary trend in Pratylenchus coffeae sensu stricto (Nematoda: Pratylenchidae): the diversity in PCR-RFLP phenotype, compatibility on host plants and reproductive segregation. Japanese Journal of Nematology 33, 57-76.
-
Moens M., Perry R.N. (2009). Migratory plant endoparasitic nematodes: a group rich in contrasts and divergence. Annual Review of Phytopathology 47, 313-332.
-
Mokrini F., Abbad Andaloussi F., Alaoui Y., Troccoli A. (2009). Importance and distribution of the main cereal nematodes in Morocco. In: Proceedings of the first workshop of the International Cereal Cyst Nematode Initiative, 21-23 October 2009, Antalya, Turkey. Ankara, Turkey, CIMMYT, pp. 45-50.
-
Mokrini F., Waeyenberge L., Viaene N., Moens M. (2012). Occurrence of nematodes of the Heterodera avenae group and Pratylenchus spp. on wheat and barley in Morocco. In: 31st international symposium of the European Society of Nematologists, 23-27 September 2012, Adana, Turkey, p. 107. [Abstr.]
-
Moody E.H., Lownsbery B.F., Ahmed J.M. (1973). Culture of the root-lesion nematode Pratylenchus vulnus on carrot disks. Journal of Nematology 19, 125-126.
-
Netscher C., Seinhorst J.W. (1969). Propionic acid better than acetic acid for killing nematodes. Nematologica 15, 286.
-
Nicol J.M. (2002). Important nematode pests. In: Curtis B.C., Rajaram S., Macpherson H.G. (Eds). Bread wheat improvement and production. Plant Production and Protection Series 30. Rome, Italy, FAO, pp. 345-366.
-
Nicol J.M., Rivoal R. (2008). Global knowledge and its application for the integrated control and management of nematodes on wheat. In: Ciancio A., Mukerji K.G. (Eds). Integrated management and biocontrol of vegetable and grain crops nematodes, Vol. 2. Dordrecht, The Netherlands, Springer, pp. 243-287.
-
Nicol J., Rivoal R., Taylor S., Zaharieva M. (2003). Global importance of cyst (Heterodera spp.) and lesion nematodes (Pratylenchus spp.) on cereals: distribution, yield loss, use of host resistance and integration of molecular tools. In: Cook, R. & Hunt, D.J. (Eds). Proceedings of the fourth international congress of nematology, 8-13 June 2002, Tenerife, Spain. Nematology Monographs and Perspectives 2 (Series Editors: Cook, R. & Hunt, D.J.). Leiden, The Netherlands, Brill, pp. 1-19.
-
Nicol J.M., Turner S.J., Coyne D.L., Nijs L.D., Hockland S., Maafi Z.T. (2011). Current nematode threats to world agriculture. In: Jones J., Gheysen G., Fennol C. (Eds). Genomics and molecular genetics of plant-nematode interactions. Dordrecht, The Netherlands, Springer, pp. 21-43.
-
Orui Y. (1996). Discrimination of the main Pratylenchus species (Nematoda: Pratylenchidae) in Japan by PCR-RFLP analysis. Applied Entomology and Zoology 31, 505-514.
-
Perry R.N., Plowright R.A., Webb R.M. (1980). Mating between Pratylenchus penetrans and P. fallax in sterile culture. Nematologica 26, 125-129.
-
Pinochet J., Verdejo A., Soler S., Canals J. (1992). Host range of a population of Pratylenchusvulnus in commercial fruit, nut, citrus, and grape rootstocks in Spain. Journal of Nematology 24, 693-698.
-
Pinochet J., Fernandez C., Esmenjaud P., Doucet M.E. (1993). Effects of six Pratylenchus vulnus isolates on the growth of peach-almond and apple rootstocks. Journal of Nematology 25, 843-861.
-
Pourjam E., Kheiri A., Geraert E. (1997). The genus Pratylenchus Filip’jev, 1936 (Tylenchida: Pratylenchidae) from north of Iran. Mededelingen Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen, Universiteit Gent 62, 741-756.
-
Rivoal R., Cook R. (1993). Nematode pests of cereals. In: Evans K., Trudgill D.L., Webster J.M. (Eds). Plant parasitic nematodes in temperate agriculture. Wallingford, UK, CABI Publishing, pp. 259-303.
-
Román J., Hirschmann H. (1969). Morphology and morphometrics of six species of Pratylenchus. Journal of Nematology 1, 363-386.
-
Ryss A.Y. (1988). World fauna of the root parasitic nematodes of the family Pratylenchidae (Tylenchida). Leningrad, USSR, Nauka.
-
Sarah J.-L., Sabatini C., Boisseau M. (1993). Differences in pathogenicity to banana (Musa sp., cv. Poyo) among populations of Radopholus similis from different production areas of the world. Nematropica 23, 75-79.
-
Seinhorst J.W. (1959). A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4, 67-69.
-
Shaner G., Stromberg E.L., Lacy G.H. (1992). Nomenclature and concepts of pathogenicity and virulence. Annual Review of Phytopathology 30, 47-66.
-
Sher S.A., Allen M.W. (1953). Revision of the genus Pratylenchus (Nematoda: Tylenchidae). University of California Publications in Zoology 57, 441-447.
-
Smiley R.W., Merrifield K., Patterson L.M., Whittaker R.G., Gourlie J.A., Easley S.A. (2004). Nematodes in dryland field crops in the semiarid Pacific Northwest United States. Journal of Nematology 36, 54-68.
-
Sogut M.A., Devran Z. (2011). Distribution and molecular identification of root-lesion nematodes in temperature fruit orchards of Turkey. Nematropica 41, 91-98.
-
Stoffelen R., Jimenez M.I., Dierckxsens C., Tam V.T.T., Swennen R., De Waele D. (1999). Effect of time and inoculum density on the reproductive fitness of 138 Pratylenchus coffeae and Radopholus similis populations on carrot disks. Nematology 1, 243-250.
-
Subbotin S.A., Ragsdale E.J., Mullens T., Roberts P.A., Mundo-Ocampo M., Baldwin J.G. (2008). A phylogenetic framework for root-lesion nematodes of the genus Pratylenchus (Nematoda): evidence from 18S and D2-D3 expansion segments of 28S ribosomal RNA genes and morphological characters. Molecular Phylogenetics and Evolution 48, 491-505.
-
Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. (2011). MEGA5: molecular evolution genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28, 2731-2739.
-
Thompson J.D., Higgins D.G., Gibson T.J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 11, 4673-4680.
-
Thompson J.P., Clewett T.G., O’Reilly M.M. (1993). Tolerance and resistance in wheat to Pratylenchus thornei. In: Pratylenchus workshop, 9th biennial conference of the Australasian Plant Pathology Society, July 1993, Hobart, Tasmania, pp. 43-48.
-
Thompson J.P., Owen K.J., Stirling G.R., Bell M.J. (2008). Root-lesion nematodes (Pratylenchus thornei and P. neglectus): a review of recent progress in managing a significant pest of grain crops in northern Australia. Australasian Plant Pathology 37, 235-242.
-
Thompson J.P., Clewett T.G., O’Reilly M.M. (2015). Temperature response of root-lesion nematode (Pratylenchus thornei) reproduction on wheat cultivars has implications for resistance screening and wheat production. Annals of Applied Biology 167, 1-10.
-
Townshend J.L. (1991). Morphological observations of Pratylenchus penetrans from celery and strawberry in southern Ontario. Journal of Nematology 23, 205-209.
-
Trinh P.Q., Wesemael W.M.L., Nguyen S.T.T., Nguyen C.N., Moens M. (2011). Pathogenicity and reproductive fitness of Pratylenchus coffeae and Radopholus arabocoffeae on arabica coffee seedlings (Coffeaarabica vs. Catimor) in Vietnam. European Journal of Plant Pathology 130, 45-57.
-
Troccoli A., De Luca F., Handoo Z.A., Di Vito M. (2008). Morphological and molecular characterization of Pratylenchus lentis n. sp. (Nematoda: Pratylenchidae) from Sicily. Journal of Nematology 40, 190-196.
-
Tuyet N.T., Elsen A., Nhi H.H., De Waele D. (2013). Effect of temperature on the in vitro reproductive fitness of Pratylenchus coffeae populations from Vietnam. Archives of Phytopathology and Plant Protection 46, 1-13.
-
Uehara T.M., Mizukubo T., Kushida A., Momota Y. (1998). Identification of P. coffeae and P. loosi using specific primers for PCR amplification of ribosomal DNA. Nematologica 44, 357-368.
-
Waeyenberge L., Ryss A., Moens M., Pinochet J., Vrain T.C. (2000). Molecular characterisation of 18 Pratylenchus species using ribosomal DNA restriction fragment length polymorphism. Nematology 2, 135-142.
-
Waeyenberge L., Viaene N., Moens M. (2009). Species-specific duplex PCR for the detection of Pratylenchus penetrans. Nematology 11, 847-857.
-
Yan G., Smiley R.W., Okubara P.A., Skantar A., Easley S.A., Sheedy J.G., Thompson A.L. (2008). Detection and discrimination of Pratylenchus neglectus and P. thornei in DNA extracts from soil. Plant Disease 92, 1480-1487.
| All Time | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 994 | 210 | 16 |
| Full Text Views | 319 | 23 | 6 |
| PDF Views & Downloads | 88 | 19 | 3 |
Diversity of root-lesion nematodes (Pratylenchus spp.) associated with wheat (Triticum aestivum and T. durum) in Morocco
In: Nematology2Institute for Agricultural and Fisheries Research, Plant, Crop Protection, Burg. Van Gansberghelaan 96, B-9820 Merelbeke, Belgium
3Laboratory for Agrozoology, Ghent University, Coupure links 653, B-9000 Ghent, Belgium
Search for other papers by Fouad Mokrini in
Current site
Google Scholar
PubMed
Search for other papers by Lieven Waeyenberge in
Current site
Google Scholar
PubMed
4Department of Biology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium
Search for other papers by Nicole Viaene in
Current site
Google Scholar
PubMed
Search for other papers by Fouad Abbad Andaloussi in
Current site
Google Scholar
PubMed
3Laboratory for Agrozoology, Ghent University, Coupure links 653, B-9000 Ghent, Belgium
Search for other papers by Maurice Moens in
Current site
Google Scholar
PubMed
Root-lesion nematodes (Pratylenchus) have a worldwide distribution and cause severe production constraints on numerous important crops. During a survey of the wheat-growing area of Morocco, 17 populations of root-lesion nematode were collected. They were identified on the basis of their morphological and morphometric characters, and by molecular methods. Microscopic observations of females and males demonstrated the occurrence of P. penetrans in 13 of the 17 samples; P. thornei and P. pseudocoffeae were detected in four samples from Zaers and a single sample from Settat, respectively. A duplex PCR primer set was used to confirm the presence of P. penetrans while the species-specific forward primer PTHO and the common reverse primer D3B were used for P. thornei. For the remaining populations, the D2-D3 expansion segments of the 28S rRNA gene were amplified and the obtained sequences were compared with those of Pratylenchus species in the GenBank database. This comparison confirmed the morphological identifications and revealed a population of P. pinguicaudatus. The study of the phylogenetic relationship of the Moroccan Pratylenchus populations showed a high similarity (99-100%) between all P. penetrans populations. The population dynamics of six Pratylenchus populations from Morocco were evaluated on carrot disk cultures at 4, 8 and 12 weeks after inoculation, and at 10, 15, 20 and 25°C. The optimum temperature for reproduction of all populations was 20°C. After 8 weeks at this temperature, nematode numbers increased up to 458-fold, 310-fold and 252-fold for the four populations of P. penetrans, the P. thornei and the P. pseudocoffeae population, respectively.
| All Time | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 994 | 210 | 16 |
| Full Text Views | 319 | 23 | 6 |
| PDF Views & Downloads | 88 | 19 | 3 |