Effect of soil and soil bacteria on hatching activity towards potato cyst nematodes (Globodera spp.)

in Nematology
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Soil samples taken from the ridge of field-grown potato (cv. British Queen) as well as from bulk soil of the same field were incubated with sterile potato root leachate or water. Samples were filtered and filtrates used in an in vitro bioassay to determine their effect on hatch of potato cyst nematodes (PCN) Globodera rostochiensis and G. pallida. Concurrently, an experiment was established where the sterile potato root leachate or water was incubated with a sterile soil wash. Ridge soil was shown to induce significantly more hatching than bulk soil, indicating the presence of PCN hatching factors. When a soil wash of ridge soil was used it did not increase hatch, suggesting a role for soil microorganisms in the hatching process. Greater hatch of G. rostochiensis in bulk soil compared to G. pallida suggests a role for soil microorganisms in spontaneous hatch of PCN in vivo.

Effect of soil and soil bacteria on hatching activity towards potato cyst nematodes (Globodera spp.)

in Nematology



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