Soil samples taken from the ridge of field-grown potato (cv. British Queen) as well as from bulk soil of the same field were incubated with sterile potato root leachate or water. Samples were filtered and filtrates used in an in vitro bioassay to determine their effect on hatch of potato cyst nematodes (PCN) Globodera rostochiensis and G. pallida. Concurrently, an experiment was established where the sterile potato root leachate or water was incubated with a sterile soil wash. Ridge soil was shown to induce significantly more hatching than bulk soil, indicating the presence of PCN hatching factors. When a soil wash of ridge soil was used it did not increase hatch, suggesting a role for soil microorganisms in the hatching process. Greater hatch of G. rostochiensis in bulk soil compared to G. pallida suggests a role for soil microorganisms in spontaneous hatch of PCN in vivo.
Detection of hatching inhibitors and hatching factor stimulants for golden potato cyst nematode, Globodera rostochiensis, in potato root leachate.
Annals of Applied Biology132463-472.
The influence of in-egg mortality and spontaneous hatching on the decline of Globodera rostochiensis during crop rotation in the absence of the host potato crop in the field.
In vitro studies on the relative availability and mobility in soil of natural hatching factors for the potato cyst nematodes, Globodera rostochiensis and G. pallida.
The influence of the hatching factor on the water uptake of the second-stage larva of the potato-cyst nematode Heterodera rostochiensis.
Journal of Experimental Biology64141-147.
The range of stimulating action of the nematode resistant cultivar Tarpan on hatching of J2 from the cysts of golden cyst nematode (Ro1 pathotype) under field conditions.
Biuletyn Instytutu Ziemniaka46104.
Comparison of the in-soil hatching responses of Globodera rostochiensis and G. pallida in the presence and absence of the host potato crop cv. British Queen.